Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,740,841	T→A	13.3%	intergenic (+78/+675)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,841	0	T	A	13.3%	142.6 / 7.0	62	intergenic (+78/+675)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base A (7/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACC  >  NC_002947/4740702‑4740989                                                                                                                                            |                                                                                                                                                      cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGaa                                                                                                                                            <  1:80569/149‑1 (MQ=255)  gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCC‑GCGAag                                                                                                                                           <  8:209077/149‑1 (MQ=255)   cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAaga                                                                                                                                          <  4:105408/149‑1 (MQ=255)    gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCC‑GCGAagag                                                                                                                                         <  5:262980/149‑1 (MQ=38)    gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAagag                                                                                                                                         <  7:215484/149‑1 (MQ=255)      cAGCTGTAGACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGc                                                                                                                                       <  3:149164/149‑1 (MQ=255)      cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGc                                                                                                                                       <  2:324644/149‑1 (MQ=255)        gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAGCGCAAGAAGGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGGCC‑GCGCAGAGGCCg                                                                                                                                     >  1:221798/1‑149 (MQ=255)          tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCgg                                                                                                                                    >  4:64655/1‑148 (MQ=255)          tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCATGGCC‑GCGAAGACGcccg                                                                                                                                    >  6:236512/1‑146 (MQ=255)          tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGAAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCgg                                                                                                                                    <  4:242734/148‑1 (MQ=255)           gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGTACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                                                                   <  1:117163/133‑1 (MQ=255)           gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                                                                   >  2:117163/1‑133 (MQ=255)           gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGCca                                                                                                                                  >  5:364303/1‑149 (MQ=255)               acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAaga                                                                                                                                          >  5:10154/1‑137 (MQ=255)               acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAaga                                                                                                                                          <  6:10154/137‑1 (MQ=255)                 cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCAGTTGGCGGGCTTGCCC‑GCGAAGAGGCCGGCACAGgca                                                                                                                            >  8:56520/1‑149 (MQ=255)                            aGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                                                                 >  7:120256/1‑149 (MQ=255)                             gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGTCCGGCACAGACACCACAAAACCt                                                                                                                 >  2:60412/1‑148 (MQ=255)                                 gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtt                                                                                                            >  6:293593/1‑149 (MQ=255)                                       gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCGTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTtctc                                                                                                      <  4:257114/149‑1 (MQ=255)                                            ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCc‑gc                                                                                                                                               >  7:116837/1‑103 (MQ=255)                                            ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCc‑gc                                                                                                                                               <  8:116837/103‑1 (MQ=255)                                               gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGACC‑GCGAAGAGGCCCGCACAGACACCACCAAACCTCAGTTTTTGTccgcccgcg                                                                                              >  4:72364/1‑148 (MQ=255)                                                 aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc                                                                                             <  5:293593/148‑1 (MQ=255)                                                                cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                             <  3:110461/149‑1 (MQ=255)                                                                cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                             <  3:64655/149‑1 (MQ=255)                                                                cTGCAGTACCCGAACGCAACACGGCTGCCCTCGGGCAG‑CATTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                                                             <  3:300756/149‑1 (MQ=255)                                                                 tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCCCAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                                                            >  1:242620/1‑149 (MQ=255)                                                                  gCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCCCAGACACCACAAAACCACAGTTTTTCTCCGCCCGCTCAAGCAGCCCGTTCAGGGt                                                                           >  6:153081/1‑149 (MQ=255)                                                                   ccgcaGCCGACCCCATCAAGGCGGCCCTGGGGCAG‑CGTTTTTTATTGGGCCGGTGCCGGCCTCTCCGCGGCCTTGCCC‑GGGATGAGGCCGGCACAGGCACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                                                          <  8:15645/145‑1 (MQ=255)                                                                      tcgCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCGTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTg                                                                       <  5:153081/147‑1 (MQ=255)                                                                           gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                                                                  >  5:128315/1‑149 (MQ=255)                                                                            aaCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTACTTGTCCTGTGCCGGCCTATTAGCGGCCATTCCC‑GCCAATAGGCCGGCACACACACCACAAAACCAAAGGTTTTATACCCCCCCGCACCCAGCCCAGTAACGGTAATGAAAGgc                                                                 >  2:136371/1‑149 (MQ=255)                                                                             aCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCCCGCAGCCCTTTCAGGGTATTGAACGgcg                                                                >  1:272020/1‑149 (MQ=255)                                                                                      ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacca                                                       <  2:56434/149‑1 (MQ=255)                                                                                          gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                                                         >  6:200346/1‑143 (MQ=255)                                                                                          gCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccac                                                         <  5:200346/143‑1 (MQ=255)                                                                                           cccTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGGCCTGGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGTGCATCCACCACGAAc                                                  <  8:17833/149‑1 (MQ=255)                                                                                           cccGCGGGCTG‑CTTTTTTTTTTTGTCGTGGGCCGCCATCTACGGCG‑CTTGCCCGGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc                                                  <  5:236512/149‑1 (MQ=255)                                                                                             cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAAc                                                  <  1:1350/147‑1 (MQ=255)                                                                                             cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt                                                <  1:305509/149‑1 (MQ=255)                                                                                             cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATCCGCCCGCTCACCCAGCCCTTTCAGGGTATTTAACGGCGCATACACCACGAACt                                                 >  2:1350/1‑148 (MQ=255)                                                                                                         ttttttATTTGTCCTGTGCCGGCCTCTTCGGGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca                                     >  1:35082/1‑149 (MQ=255)                                                                                                         ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacc                                      >  2:250906/1‑148 (MQ=255)                                                                                                                        gTGCCGGCCTCTTCGCGGCCTTTCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCGTTCAGGGGATTTAACGGTGCATCCACCACGAACTTGTTTGCCCCCATAGCCGGCACGCGac                      >  6:291250/1‑149 (MQ=255)                                                                                                                             ggCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCt                 >  2:323394/1‑149 (MQ=255)                                                                                                                              gCCGCTTCCCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTg                <  8:257290/149‑1 (MQ=255)                                                                                                                                ctctTCGCGGGCAAGGCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGc              >  4:288944/1‑149 (MQ=255)                                                                                                                                 tctTCGCGGGCAAGGCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt             >  5:13654/1‑149 (MQ=255)                                                                                                                                 tctTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCt             >  2:259863/1‑149 (MQ=255)                                                                                                                                  ctTCGCGGGCAAGGCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTc            >  3:114764/1‑149 (MQ=255)                                                                                                                                   ttCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg           >  5:322662/1‑149 (MQ=255)                                                                                                                                     cgcgGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCg           <  6:322662/147‑1 (MQ=255)                                                                                                                                     cgcgGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt         >  5:82874/1‑149 (MQ=255)                                                                                                                                     cgcgGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgt         >  5:77545/1‑149 (MQ=255)                                                                                                                                       cgcccTTCCCC‑GCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgtgt       <  1:147418/146‑1 (MQ=255)                                                                                                                                       cgGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAATTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgtgt       <  1:259863/149‑1 (MQ=255)                                                                                                                                       cgGCCTGGCCC‑GCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGgtgt       <  1:136371/149‑1 (MQ=255)                                                                                                                                         gCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGc     <  1:250906/149‑1 (MQ=255)                                                                                                                                         gCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGc     <  8:120256/149‑1 (MQ=255)                                                                                                                                           gttGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCAc   <  3:72364/148‑1 (MQ=255)                                                                                                                                           cTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCAACGCCGGCACGCTACCACCTGGCTCGGTGTGCAc   >  4:171974/1‑149 (MQ=255)                                                                                                                                            ttGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                                                           <  6:231676/92‑1 (MQ=255)                                                                                                                                            ttGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcc                                                           >  5:231676/1‑92 (MQ=255)                                                                                                                                            ttGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCAcc  >  8:219536/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                      CGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCC‑GCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCGCCGGCACGCTACCACCTGGCTCGGTGTGCACC  >  NC_002947/4740702‑4740989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑