Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,744,607	G→T	6.8%	G73G (GGC→GGA)	PP_4198 ←	OmpA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,744,607	0	G	T	6.8%	212.7 / 3.4	68	G73G (GGC→GGA)	PP_4198	OmpA family protein
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base T (2/3);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGA  >  NC_002947/4744460‑4744754                                                                                                                                                     |                                                                                                                                                     ggCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGc                                                                                                                                                     >  2:176130/1‑149 (MQ=255)  gCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGCACCGGTTcc                                                                                                                                                    <  4:145629/149‑1 (MQ=255)       aCGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGAt                                                                                                                                                 <  8:356324/147‑1 (MQ=255)       aCGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATg                                                                                                                                                <  2:1993/148‑1 (MQ=255)         gCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCGGAGGTAAGCCTAATGCCG‑CACATCGGCAGCGGTGGCGAGGtcg                                                                                                                                             >  4:79612/1‑147 (MQ=255)            aGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTg                                                                                                                                          <  1:206851/149‑1 (MQ=255)             ggCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTg                                                                                                                                          >  2:71668/1‑148 (MQ=255)               ccAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGa                                                                                                                                        >  2:49249/1‑148 (MQ=255)                cAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGa                                                                                                                                        >  7:205294/1‑147 (MQ=255)                 aGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAg                                                                                                                                     <  4:173435/149‑1 (MQ=255)                  gCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAgg                                                                                                                                    <  2:306351/149‑1 (MQ=255)                      cAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGgcg                                                                                                                                  <  6:4457/147‑1 (MQ=255)                         gcgctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑gg                                                                                                                            <  7:14380/149‑1 (MQ=255)                            ctggcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑Ggcgc                                                                                                                         <  2:120297/149‑1 (MQ=255)                               gcgctCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGa                                                                                                                       <  8:205294/148‑1 (MQ=255)                                     cAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGc                                                                                                                 <  1:176130/148‑1 (MQ=255)                                       ggTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGAc                                                                                                              >  3:297615/1‑149 (MQ=255)                                         tCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACt                                                                                                             >  4:196840/1‑148 (MQ=255)                                            aGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCAcc                                                                                                         <  4:15590/149‑1 (MQ=255)                                                    cTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGaa                                                                                                 >  7:293977/1‑149 (MQ=255)                                                       gcgcTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAAtca                                                                                              >  2:187064/1‑149 (MQ=255)                                                         gcTCCTGGTTCAGCGCCAGCTTGGCATTCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTCTGGCGCGAGCCAGCGACTCACCCGCCCGAAtcac                                                                                             <  1:49249/149‑1 (MQ=255)                                                                  tCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACgcggc                                                                                   >  8:10239/1‑149 (MQ=255)                                                                     gcgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGgcgcg                                                                                >  4:73197/1‑149 (MQ=255)                                                                      cgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTGGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCa                                                                                                           >  1:157203/1‑121 (MQ=255)                                                                      cgcCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTGGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCa                                                                                                           <  2:157203/121‑1 (MQ=255)                                                                         cAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgc                                                                            >  8:244468/1‑149 (MQ=255)                                                                          aGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgcg                                                                           <  3:196840/149‑1 (MQ=255)                                                                          aGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTgcg                                                                           <  8:293977/149‑1 (MQ=255)                                                                                  aTGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGCATCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACAt                                                                   >  7:104393/1‑149 (MQ=255)                                                                                        gggggCAATCTCGCTGTATCGCTGGGTGATGGAATCATAATGCCGCCTAAAGGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCg                                                                 <  1:121623/144‑1 (MQ=255)                                                                                         cgGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAat                                                             >  7:141230/1‑148 (MQ=255)                                                                                            gCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCAGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCTCAGCACATCGGAatcatc                                                         >  1:103275/1‑149 (MQ=255)                                                                                              aaTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGaa                                                              <  8:258092/142‑1 (MQ=255)                                                                                              aaTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGaa                                                              >  7:258092/1‑142 (MQ=255)                                                                                               aTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCttt                                                      <  4:297615/149‑1 (MQ=255)                                                                                               aTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCttt                                                      <  7:10239/149‑1 (MQ=255)                                                                                               aTCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCt                                                        >  1:131696/1‑147 (MQ=255)                                                                                                 ctcGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGa                                                    >  1:185574/1‑149 (MQ=255)                                                                                                      tGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGAccccc                                               >  5:155759/1‑149 (MQ=255)                                                                                                        tAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTg                                             >  1:204472/1‑149 (MQ=255)                                                                                                          gcgcTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTgg                                            <  1:187064/148‑1 (MQ=255)                                                                                                          gcgcTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTgg                                            >  5:110095/1‑148 (MQ=255)                                                                                                             ctggctgAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAgg                                        <  5:315352/149‑1 (MQ=255)                                                                                                                  tgAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCt                                                        <  3:194386/128‑1 (MQ=255)                                                                                                                  tgAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCt                                                        >  4:194386/1‑128 (MQ=255)                                                                                                                       tAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCTGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGc                              <  1:71668/149‑1 (MQ=255)                                                                                                                       tAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGc                              >  5:235463/1‑149 (MQ=255)                                                                                                                        aaGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGcc                             >  7:9301/1‑149 (MQ=255)                                                                                                                          gCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCt                            >  5:311139/1‑148 (MQ=255)                                                                                                                          gCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCt                            <  6:311139/148‑1 (MQ=255)                                                                                                                           cATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCCCCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTc                           >  7:170838/1‑148 (MQ=255)                                                                                                                              aaTGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATc                        >  4:219685/1‑148 (MQ=255)                                                                                                                              aaTGCCG‑CACATCGGAACCGGTGCCGATGAAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATc                        >  5:205332/1‑148 (MQ=255)                                                                                                                                   cG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAgcgc                  <  5:20063/149‑1 (MQ=255)                                                                                                                                    gttacaTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTg                                >  6:241147/4‑135 (MQ=255)                                                                                                                                    gttacaTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTg                                <  5:241147/132‑1 (MQ=255)                                                                                                                                    gttacaTCGGCACCGGTTCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAgcgc                  >  4:266772/4‑149 (MQ=255)                                                                                                                                    g‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGcgcg                 <  2:131696/149‑1 (MQ=255)                                                                                                                                      cacaTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCgg                >  5:222339/1‑149 (MQ=255)                                                                                                                                         aTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAc             >  5:60972/1‑149 (MQ=255)                                                                                                                                         aTCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAAGCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGCCCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCAc             >  8:288464/1‑149 (MQ=255)                                                                                                                                            ggAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgc          >  1:221014/1‑149 (MQ=255)                                                                                                                                              aaCCGGTTCCGATGTAACTGGACAGGCGCGC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCTCGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGTCCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgctg        <  8:104393/149‑1 (MQ=255)                                                                                                                                              aaCCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCActgctg        <  8:267357/149‑1 (MQ=255)                                                                                                                                                  ggTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCt    <  8:141230/149‑1 (MQ=255)                                                                                                                                                   gTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCAGCAGTGCCGCGCTg   <  2:204472/149‑1 (MQ=255)                                                                                                                                                    tGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGa  <  6:110095/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGCCTCACGCAAGGCCAGCTGCAGGCGCTGGCGCTCCAGGTCGAGCTTGGCCTGGCGCTCCTGGTTCAGCGCCAGCTTGGCATGCTCGCGGGCAATCTCGCTGTAGCGCTGGCTGAGGTAAGCATAATGCCG‑CACATCGGAACCGGTGCCGATGTAACTGGACAGGCGCTC‑GGCGCGAGCCAGCGACTCACCCGCCCGAATCACGTCACGCGGCGCGCTGCGCAGCACATCGGAATCATCTTTGACCCCCTGGAAGGCAACGGTGGCCTCATCCAGCGCGGCACTGCTGCGCTGA  >  NC_002947/4744460‑4744754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑