Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,767,819 A→G 8.3% intergenic (+33/+36) fpvA → / ← PP_4218 TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,767,8190AG 8.3% 243.1 / 9.5 84intergenic (+33/+36)fpvA/PP_4218TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein
Reads supporting (aligned to +/- strand):  ref base A (34/43);  new base G (4/3);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CAACGTATTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCACCGCAAGC  >  NC_002947/4767672‑4767966
                                                                                                                                                     |                                                                                                                                                   
caacGTATTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAg                                                                                                                                                    >  8:262115/1‑149 (MQ=255)
    gTATTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGAAGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAATTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGc                                                                                                                                                <  1:68375/149‑1 (MQ=255)
     tATTCGACAAGAAGTACTACCAGGGCAATGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATAGAGAGACCCACGCAACTTCACAATCACCGCCAAGTACAGCTTCAGATCGCCTGCCGTTGAACGCAAAAAACCGCACCCAGGTg                                                                                                                                                 >  1:111455/1‑149 (MQ=255)
          gACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttt                                                                                                                                          >  3:8032/1‑149 (MQ=255)
          gACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGtttt                                                                                                                                          <  6:185339/149‑1 (MQ=255)
          gACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGtttt                                                                                                                                          >  2:10302/1‑149 (MQ=255)
            cAAGAAGTACTACTAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt                                                                                                                                        <  5:235998/149‑1 (MQ=255)
            cAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt                                                                                                                                        <  4:8032/149‑1 (MQ=255)
            cAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt                                                                                                                                        <  6:143391/149‑1 (MQ=255)
            cAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGttttt                                                                                                                                         >  2:320729/1‑148 (MQ=255)
             aagaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGtttttt                                                                                                                                        >  5:214229/1‑148 (MQ=255)
             aagaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGtttttt                                                                                                                                        >  4:298317/1‑148 (MQ=255)
               gaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCAGGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCGAGGTGCGGTTTTTTCAt                                                                                                                                     <  4:278044/149‑1 (MQ=255)
               gaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCAt                                                                                                                                     <  8:145751/149‑1 (MQ=255)
               gaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCAt                                                                                                                                     <  4:167077/149‑1 (MQ=255)
                        ccAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGcc                                                                                                                              <  1:320729/147‑1 (MQ=255)
                         cAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACAGCACCCAGGTGCGGTTTTTTAATGGATGCCTg                                                                                                                            >  6:299098/1‑148 (MQ=255)
                          aGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGa                                                                                                                           >  7:22837/1‑148 (MQ=255)
                           ggtcATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGaaa                                                                                                                         <  8:148233/146‑1 (MQ=255)
                             gCATTGCCAGCAACTACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAgg                                                                                                                       <  2:111455/149‑1 (MQ=255)
                             gCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAgg                                                                                                                       <  8:22837/149‑1 (MQ=255)
                             gCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAgg                                                                                                                       <  7:262115/149‑1 (MQ=255)
                              cATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAgg                                                                                                                       >  8:149629/1‑148 (MQ=255)
                              cATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGc                                                                                                                      <  2:189041/149‑1 (MQ=255)
                               aTTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCt                                                                                                                     <  7:161214/149‑1 (MQ=255)
                                  gCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt                                                                                                                                                  >  6:322684/1‑117 (MQ=255)
                                  gCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt                                                                                                                                                  <  5:322684/117‑1 (MQ=255)
                                   ccAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCa                                                                                                                                      <  6:5890/128‑1 (MQ=255)
                                   ccAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTccc                                                                                                                  <  4:164134/148‑1 (MQ=255)
                                   ccAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCt                                                                                                                 <  1:194915/149‑1 (MQ=255)
                                     aGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTa                                                                                                               >  3:64082/1‑149 (MQ=255)
                                       caactaCTCCTGGAGCCCGTACGACGTGTAT‑T‑GTTTCCCACGCAACTTCACCATCTCCGCCAAGTACAGCTTCTGATCGCCTGACGTTGTACGCAAAAAACCGCAACCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACa                                                                                                             <  7:188786/149‑1 (MQ=255)
                                       caacaaCTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACa                                                                                                             <  5:15251/149‑1 (MQ=255)
                                          caacTCCTGGAGACCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACAATCACCGCCAAGTAAACCTTCTGATCGCCTGACGATGAACGCAAAAAAACGCACCCAGGTCCGGTTGTTGCATGGATGCCTGAAAGGCGCCCTTACAGAt                                                                                                          >  4:288329/1‑149 (MQ=255)
                                           aacTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtg                                                                                                         >  5:44093/1‑149 (MQ=255)
                                              tCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctg                                                                                                      >  2:257807/1‑149 (MQ=255)
                                                      cccGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTg                                                                                              >  3:289010/1‑149 (MQ=255)
                                                                   tAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCAt                                                                                 >  8:260139/1‑149 (MQ=255)
                                                                    aT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATc                                                                                >  4:166913/1‑149 (MQ=255)
                                                                     t‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATc                                                                                <  5:135658/148‑1 (MQ=255)
                                                                     t‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATc                                                                                <  3:166913/148‑1 (MQ=255)
                                                                     t‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCg                                                                               >  2:372412/1‑149 (MQ=255)
                                                                       g‑gTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGc                                                                              >  3:186821/1‑149 (MQ=255)
                                                                            aCCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAgg                                                                          <  3:298317/149‑1 (MQ=255)
                                                                             cccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGc                                                                         <  1:10302/149‑1 (MQ=255)
                                                                              ccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGcc                                                                        >  7:288898/1‑149 (MQ=255)
                                                                               cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt                                                                       <  4:310998/149‑1 (MQ=255)
                                                                               cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt                                                                       >  6:237813/1‑149 (MQ=255)
                                                                               cACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGCTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt                                                                       >  4:173912/1‑149 (MQ=255)
                                                                                aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt                                                                       >  6:326611/1‑148 (MQ=255)
                                                                                    aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATc                                                                  <  1:372412/149‑1 (MQ=255)
                                                                                    aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATc                                                                  <  7:22264/149‑1 (MQ=255)
                                                                                     aCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCg                                                                 <  4:289010/149‑1 (MQ=255)
                                                                                        tCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATc                                                                  <  2:38078/145‑1 (MQ=255)
                                                                                        tCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATc                                                                  >  1:38078/1‑145 (MQ=255)
                                                                                          aCCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctc                                                            <  3:173912/149‑1 (MQ=255)
                                                                                          aCCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctc                                                            >  5:45752/1‑149 (MQ=255)
                                                                                          aCCATCACCGCCAACTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGCATGACTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctc                                                            >  3:187904/1‑149 (MQ=255)
                                                                                            cATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcct                                                          <  6:214229/149‑1 (MQ=255)
                                                                                             aTCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCctcctc                                                         <  8:288898/149‑1 (MQ=255)
                                                                                                    ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  <  1:257807/149‑1 (MQ=255)
                                                                                                    ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  <  5:237813/149‑1 (MQ=255)
                                                                                                    ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  <  7:149629/149‑1 (MQ=255)
                                                                                                    ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  >  2:7514/1‑149 (MQ=255)
                                                                                                       aGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  <  1:205212/146‑1 (MQ=255)
                                                                                                       aGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                                  >  2:205212/1‑146 (MQ=255)
                                                                                                       aGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGaa                                               <  1:297206/149‑1 (MQ=255)
                                                                                                        gTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc                                              <  6:44093/149‑1 (MQ=255)
                                                                                                           cAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCa                                           >  4:279721/1‑149 (MQ=255)
                                                                                                                  tGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCAt                                    <  7:260139/149‑1 (MQ=255)
                                                                                                                     tCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATg                                   >  8:323351/1‑147 (MQ=255)
                                                                                                                      cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAg                                >  6:266249/1‑149 (MQ=255)
                                                                                                                           gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg                           >  5:56468/1‑149 (MQ=255)
                                                                                                                           gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg                           >  3:131919/1‑149 (MQ=255)
                                                                                                                            aCGTTGAACTCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATgg                          <  5:299098/149‑1 (MQ=255)
                                                                                                                            aCGTTGAACGCAAAAAAGCGCACCCAGGTGCGGTTTTTTCATGGATGCGTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCTTCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg                           <  4:187904/148‑1 (MQ=255)
                                                                                                                            aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg                           <  6:45752/148‑1 (MQ=255)
                                                                                                                                    atgaaaaaaCCGCACCTGGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTcc                  >  4:299003/4‑149 (MQ=255)
                                                                                                                                         aaaaCCGCACCTGGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCggg              >  2:376218/1‑148 (MQ=255)
                                                                                                                                           aaCCGCACCTGGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGAt            >  7:88036/1‑148 (MQ=255)
                                                                                                                                             ccGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCAc         <  6:56468/149‑1 (MQ=255)
                                                                                                                                             ccGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCAc         >  7:76832/1‑149 (MQ=255)
                                                                                                                                              cGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCAcc        <  1:7514/149‑1 (MQ=255)
                                                                                                                                                    cAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCACCGCAAGc  <  5:326611/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAACGTATTCGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTAT‑G‑GTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGATCACCGCAAGC  >  NC_002947/4767672‑4767966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑