| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,779,409 | T→G | 7.4% | intergenic (‑143/+18) | pvdD ← / ← pvdJ | non‑ribosomal peptide synthetase/non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,779,409 | 0 | T | G | 7.4% | 156.1 / 4.7 | 54 | intergenic (‑143/+18) | pvdD/pvdJ | non‑ribosomal peptide synthetase/non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base T (24/26); new base G (2/2); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
ACTTGAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAGG > NC_002947/4779265‑4779557 | aCTTGAACATCTCCTACCCGGGCACCGGTGTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGGTCATTATGGTGCCGAACAAATGAAATCTGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATAc < 1:102929/148‑1 (MQ=255) cTTGAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGGCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCgg < 3:191452/149‑1 (MQ=255) aCATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCg < 8:39720/149‑1 (MQ=255) aCATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTATGACCGGGccc < 3:123603/148‑1 (MQ=255) cATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTATGACCGGGCCCgg < 1:128685/149‑2 (MQ=255) tctcCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCg > 6:276956/1‑146 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCaga < 4:24582/146‑1 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCaga < 5:328532/146‑1 (MQ=255) ggCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTgg < 7:264343/149‑1 (MQ=255) ggCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTgg < 5:226345/149‑1 (MQ=255) ggTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAAc < 5:276956/149‑1 (MQ=255) gCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTTCTGGGTCAACTCGTCGAtctc > 5:190146/1‑149 (MQ=255) aGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAg > 6:261923/1‑149 (MQ=255) gCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTGATACCGGGCCCGCAGAGCGCTACGGGGTCAACTCGTCGCTCTCCGCTGCAGTAAGCCGGTTCAAggc > 4:8644/1‑149 (MQ=255) gCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggc < 6:190146/149‑1 (MQ=255) gCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggc > 5:221424/1‑149 (MQ=255) cATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcggc > 2:246366/1‑149 (MQ=255) atgatgCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcggc > 7:248189/1‑148 (MQ=255) atgatgCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGGTCACGACCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcgg < 3:42929/147‑1 (MQ=255) ccGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGAtt > 7:46157/1‑147 (MQ=255) ccGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa < 2:267129/149‑1 (MQ=255) ccGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa < 8:248189/149‑1 (MQ=255) ccGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAAGAtt > 8:99210/1‑147 (MQ=255) aaCAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGcc < 5:261923/149‑1 (MQ=255) ccGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt > 2:12052/1‑149 (MQ=255) cTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCa < 4:279073/94‑1 (MQ=255) cTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCa > 3:279073/1‑94 (MQ=255) ctGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 4:209550/72‑1 (MQ=255) ctGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc > 3:209550/1‑72 (MQ=255) ggTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCtt < 4:160391/146‑1 (MQ=255) gtgtACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCaa < 6:21694/149‑1 (MQ=255) tGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGc > 3:362721/1‑149 (MQ=255) tGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGc > 5:283124/1‑149 (MQ=255) tGAAGTTGACGAGCCGGGCCCCGTCATACCGGGCCCCCAGAGCGCAACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGAATTAGACAACACGTGCTGAATCGACCCGCCCTGCCCTTGCCACACTGc > 4:29535/1‑149 (MQ=255) aaGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGa > 7:64367/1‑149 (MQ=255) aaGTGGACTAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAGGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGa < 2:279783/149‑1 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgc < 6:101440/128‑1 (MQ=255) gACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgc > 5:101440/1‑128 (MQ=255) ctctgcGGGCCCGGTATGACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTc > 1:315038/6‑149 (MQ=255) cGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTg < 2:54646/64‑1 (MQ=255) cGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTc < 6:352225/149‑1 (MQ=255) gAGCCGGGCCCGGTCATAACGGGCCCGCAGAGCGCTACAGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACCTTTCAAGGCGGCCAAGGATTTAGCCAACACGTCCTGAAAAGACGCGCCCTGCCCTTTCAACAATGCGACGGCCACg > 8:16236/1‑149 (MQ=255) gAGCCGGGCCCGGGCATACCGGGCCCGCAGAGCGCGTCTGGGTCAACTCGTCGCTCTGCTCGGCAGGAAGACGGTTCAAGGCGGCCAAGGCTTTAGCCCACTCGTGCTGCATGGCCGCGCCCTGCCCGGGGAACACAGCGCCGGCCTCg > 6:22223/1‑149 (MQ=255) ccGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTa > 2:210266/1‑92 (MQ=255) ccGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGAAAGACGTTTCAAGGCGGCCAAGGATTTa < 1:210266/92‑1 (MQ=255) cGGGCCCGGTCATACCGGGCCCGCACAGCGCTACTGGCTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTg > 1:196953/1‑148 (MQ=255) ggCCCGGTATGACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGacc > 5:215548/1‑147 (MQ=255) ccGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCtt < 2:205439/120‑1 (MQ=255) ccGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGGTTCAAGGCGGCCAAGGATTTAGCCCACTCGTCCTGAATCGACGCGCCCTGCCCtt > 1:205439/1‑120 (MQ=255) cGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTc < 1:12052/149‑1 (MQ=255) ggTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCGCTTGCAACACTGCGACGGCCTCGCTGAACGCTcc > 8:54138/1‑149 (MQ=255) aTACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCg > 4:280639/1‑134 (MQ=255) aTACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCg < 3:280639/134‑1 (MQ=255) aTACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCACTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCaa < 8:64367/148‑1 (MQ=255) tACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAgg > 5:347485/1‑149 (MQ=255) | ACTTGAACATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAA‑GAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACACTGCGACGGCCTCGCTGAACGCTCCCAAGG > NC_002947/4779265‑4779557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |