| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,780,957 | T→C | 7.0% | Q2119Q (CAA→CAG) | pvdJ ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,780,957 | 0 | T | C | 7.0% | 167.6 / 2.8 | 57 | Q2119Q (CAA→CAG) | pvdJ | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base T (24/29); new base C (2/2); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGC > NC_002947/4780812‑4781104 | cGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTgg < 1:344218/149‑1 (MQ=255) cGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGcc > 3:174152/1‑148 (MQ=255) gAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGcc > 3:241783/1‑147 (MQ=255) gAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCaa < 7:233130/149‑1 (MQ=255) aGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCa < 8:234564/149‑1 (MQ=255) gAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAg < 1:31986/149‑1 (MQ=255) aaGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAgg < 1:253558/149‑1 (MQ=255) aGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAgg < 3:19588/148‑1 (MQ=255) aGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGGCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGc < 7:198444/149‑1 (MQ=255) gTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGc > 7:225786/1‑148 (MQ=255) aaGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAAt < 2:347112/148‑1 (MQ=255) aaGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATg > 8:296662/1‑149 (MQ=255) aGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGc < 2:33320/149‑1 (MQ=255) aTCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATTGCCTGGGCTTGGCCCAAGCGATGCTc < 4:96010/149‑1 (MQ=255) tCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGTCGCGGCGTGTGCCTGCAGCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATTGCCTGGGCTTGGCCCAAGCGATGCTcg < 8:164508/149‑2 (MQ=255) tAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGca > 5:383386/1‑149 (MQ=255) cagcagCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCacac > 6:118689/1‑147 (MQ=255) agcagcTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAg > 5:216651/1‑149 (MQ=255) agcagcTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAg < 5:151728/149‑1 (MQ=255) ttGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCcg > 7:41332/1‑149 (MQ=255) cggcATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGcac < 2:24037/134‑1 (MQ=255) cggcATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGcac > 1:24037/1‑134 (MQ=255) aTTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGAACCTGCTCCCAGACCAACAAGGTCGGCGGAGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACAAGCACCCGCGCCTGGc > 7:47172/1‑149 (MQ=255) ttGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCt < 7:295595/149‑1 (MQ=255) tGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCaa < 2:23366/148‑1 (MQ=255) gCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAgcgc < 8:225786/149‑1 (MQ=255) gCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAgcg > 1:272070/1‑148 (MQ=255) aGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCgcag > 3:317593/1‑149 (MQ=255) tgCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAg < 6:362465/147‑1 (MQ=255) ccGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgc < 4:317593/149‑1 (MQ=255) ccGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgc < 7:296662/149‑1 (MQ=255) gtgtGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGc > 2:322070/1‑148 (MQ=255) tgCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAgg > 4:104724/1‑149 (MQ=255) cACCTGCTCCCAGACCAACACGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGa < 6:357020/148‑1 (MQ=255) cACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGAt < 5:118689/149‑1 (MQ=255) cACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGAt > 4:65924/1‑149 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGCAGCCTTCGAGCATCGCTTGGGCCAAGACCAGGCAATGCTCATCGCCCACCAGCACCCGCGCCTGGCGCAAGCGCAGCACATCGGCCAGGCGCGCCCCCGGCAAGGACGGATc > 3:359002/1‑147 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAg > 6:304011/1‑106 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAg < 5:304011/106‑1 (MQ=255) cTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTGCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGAACACCAGCACCCGCGCGTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAg < 7:246433/147‑1 (MQ=255) ccAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCgaga < 7:213489/149‑1 (MQ=255) cAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 8:213489/1‑146 (MQ=255) aGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 2:279014/1‑145 (MQ=255) aGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 2:221483/1‑145 (MQ=255) ccAACACGGGGGGGGGCGCTGGTAAGCCTGCGCGCATCGCTTGGGCCAACCCCAGGAAATGCTCATCCCACCCCCGCCCCCGCGCCGGGCTCAAGCGCAGCACATCGGCCTGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATaa < 8:41332/149‑1 (MQ=255) ggcggcGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgcg > 6:286831/1‑106 (MQ=255) ggcggcGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgcg < 5:286831/106‑1 (MQ=255) cggcgCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCt < 6:383386/149‑1 (MQ=255) gcTTGGAACCGTTCGTGCATCGTTTGGGCAACGCCAAGTAATTGCTCATCGCACACAACCACCCGCGCCTGGCTCACGCCCAGCACAGCGGCCCGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGaa < 8:47172/149‑1 (MQ=255) ttGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAaca > 7:86849/1‑149 (MQ=255) tGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAacac > 4:213167/1‑149 (MQ=255) aaGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACAcccc < 2:272070/149‑1 (MQ=255) ttCGAGCATCGCTTGGGCCAAGCCCAGGCAATGATCATCGCACACCAGCACACCCGCCTGGCTCAAGCGCAGCACAACGGCCAGCAGCCCCACCCGCAAGGACGGATCCAGCGACAGATAACCCGCACCAGCCAGGAACACCCCGacca > 5:55221/1‑149 (MQ=255) tgAGCATTGCCTGGGCTTGGCCCAAGCGATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGaccac > 2:291514/2‑148 (MQ=255) cGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGacca < 1:279014/147‑1 (MQ=255) gAGCATTGCCTGGGCTTGGCCCAAGCGATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCCGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGAccacca > 3:97636/1‑149 (MQ=255) cATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGc < 3:213167/149‑1 (MQ=255) ttGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGc > 5:103007/1‑148 (MQ=255) | CGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGC > NC_002947/4780812‑4781104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |