| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,780,974 | A→G | 6.9% | C2114R (TGC→CGC) | pvdJ ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,780,974 | 0 | A | G | 6.9% | 171.3 / 2.5 | 58 | C2114R (TGC→CGC) | pvdJ | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (26/28); new base G (2/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
AAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGTCACGCTC > NC_002947/4780829‑4781121 | aaGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAAt < 2:347112/148‑1 (MQ=255)aaGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATg > 8:296662/1‑149 (MQ=255) aGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGc < 2:33320/149‑1 (MQ=255) aTCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATTGCCTGGGCTTGGCCCAAGCGATGCTc < 4:96010/149‑1 (MQ=255) tCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGTCGCGGCGTGTGCCTGCAGCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATTGCCTGGGCTTGGCCCAAGCGATGCTcg < 8:164508/149‑2 (MQ=255) tAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGca > 5:383386/1‑149 (MQ=255) cagcagCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCacac > 6:118689/1‑147 (MQ=255) agcagcTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAg < 5:151728/149‑1 (MQ=255) agcagcTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAg > 5:216651/1‑149 (MQ=255) ttGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCcg > 7:41332/1‑149 (MQ=255) cggcATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGcac < 2:24037/134‑1 (MQ=255) cggcATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGcac > 1:24037/1‑134 (MQ=255) aTTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGAACCTGCTCCCAGACCAACAAGGTCGGCGGAGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACAAGCACCCGCGCCTGGc > 7:47172/1‑149 (MQ=255) ttGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCt < 7:295595/149‑1 (MQ=255) tGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCaa < 2:23366/148‑1 (MQ=255) gCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAgcgc < 8:225786/149‑1 (MQ=255) gCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAgcg > 1:272070/1‑148 (MQ=255) aGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCgcag > 3:317593/1‑149 (MQ=255) tgCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAg < 6:362465/147‑1 (MQ=255) ccGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgc < 4:317593/149‑1 (MQ=255) ccGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgc < 7:296662/149‑1 (MQ=255) gtgtGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGc > 2:322070/1‑148 (MQ=255) tgCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAgg > 4:104724/1‑149 (MQ=255) cACCTGCTCCCAGACCAACACGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGa < 6:357020/148‑1 (MQ=255) cACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGAt > 4:65924/1‑149 (MQ=255) cACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGAt < 5:118689/149‑1 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGCAGCCTTCGAGCATCGCTTGGGCCAAGACCAGGCAATGCTCATCGCCCACCAGCACCCGCGCCTGGCGCAAGCGCAGCACATCGGCCAGGCGCGCCCCCGGCAAGGACGGATc > 3:359002/1‑147 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAg < 5:304011/106‑1 (MQ=255) cTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAg > 6:304011/1‑106 (MQ=255) cTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTGCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGAACACCAGCACCCGCGCGTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAg < 7:246433/147‑1 (MQ=255) ccAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCgaga < 7:213489/149‑1 (MQ=255) cAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 8:213489/1‑146 (MQ=255) aGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 2:221483/1‑145 (MQ=255) aGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCga > 2:279014/1‑145 (MQ=255) ccAACACGGGGGGGGGCGCTGGTAAGCCTGCGCGCATCGCTTGGGCCAACCCCAGGAAATGCTCATCCCACCCCCGCCCCCGCGCCGGGCTCAAGCGCAGCACATCGGCCTGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATaa < 8:41332/149‑1 (MQ=255) ggcggcGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgcg < 5:286831/106‑1 (MQ=255) ggcggcGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGgcgcg > 6:286831/1‑106 (MQ=255) cggcgCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCt < 6:383386/149‑1 (MQ=255) gcTTGGAACCGTTCGTGCATCGTTTGGGCAACGCCAAGTAATTGCTCATCGCACACAACCACCCGCGCCTGGCTCACGCCCAGCACAGCGGCCCGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGaa < 8:47172/149‑1 (MQ=255) ttGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAaca > 7:86849/1‑149 (MQ=255) tGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAacac > 4:213167/1‑149 (MQ=255) aaGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACAcccc < 2:272070/149‑1 (MQ=255) ttCGAGCATCGCTTGGGCCAAGCCCAGGCAATGATCATCGCACACCAGCACACCCGCCTGGCTCAAGCGCAGCACAACGGCCAGCAGCCCCACCCGCAAGGACGGATCCAGCGACAGATAACCCGCACCAGCCAGGAACACCCCGacca > 5:55221/1‑149 (MQ=255) tgAGCATTGCCTGGGCTTGGCCCAAGCGATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGaccac > 2:291514/2‑148 (MQ=255) cGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGacca < 1:279014/147‑1 (MQ=255) gAGCATTGCCTGGGCTTGGCCCAAGCGATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCCGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGAccacca > 3:97636/1‑149 (MQ=255) cATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGc < 3:213167/149‑1 (MQ=255) ttGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGc > 5:103007/1‑148 (MQ=255) gCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGa > 4:220815/1‑149 (MQ=255) ccAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGa > 4:184474/1‑148 (MQ=255) cAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACa < 1:322070/149‑1 (MQ=255) cAAGCCCAGGCAATGCTCATCGCACACCACCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACa < 8:279384/149‑1 (MQ=255) aaaGCCCAGGAAATGCTATGCCCACCCCCCCCCCGGCCCCTGGCTCACGCGCAGAACATCGCCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACa < 6:216651/148‑1 (MQ=255) aaGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAg > 1:283216/1‑149 (MQ=255) gCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGt < 6:103007/149‑1 (MQ=255) cccAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGTc < 3:220815/149‑1 (MQ=255) cggCAATGCTCAGCGCACCCCAGCACCCCCGCCTGGCTCAAGCCCAGCACAGCGGCCTGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGTCACg < 2:283216/148‑1 (MQ=255) cAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGTTCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGTCACGCTc > 1:117636/1‑149 (MQ=255) | AAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGGGCAGGTGCAGCATCTGTGCCGGCGCGGCGTGTGCCTGCACCTGCTCCCAGACCAACAAGGTCGGCGGCGCTTGGAAGCCTTCGAGCATCGCTTGGGCCAAGCCCAGGCAATGCTCATCGCACACCAGCACCCGCGCCTGGCTCAAGCGCAGCACATCGGCCAGGCGCGCCACCGGCAAGGACGGATCCAGCGAGAGATAACCGGCACCCGCCTGGAACACCCCGACCACCATGCCCAGCAACGACAGGTCACGCTC > NC_002947/4780829‑4781121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |