| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,796,614 | G→C | 15.4% | intergenic (‑172/+46) | pvdH ← / ← PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,796,614 | 0 | G | C | 15.4% | 125.3 / 15.5 | 52 | intergenic (‑172/+46) | pvdH/PP_4224 | diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (20/24); new base C (5/3); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01 | |||||||||||
GGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCA > NC_002947/4796466‑4796760 | ggCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAcccgcccg > 3:263586/1‑149 (MQ=255) aGCGGTGGACATGGGTAAATCCTCGCGAGCAAGCGGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAg > 1:61298/1‑149 (MQ=255) aGCGGTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGc < 5:377647/149‑1 (MQ=255) ggTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCggg < 5:258490/149‑1 (MQ=255) gTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGt > 8:1057/1‑149 (MQ=255) gACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCa < 2:268594/149‑1 (MQ=255) aCATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTAc < 3:341712/149‑1 (MQ=38) aCATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGCCTGTGCTGGCCTCTTCGGAGGTGAACCCGCTCCTACGGGCGGGTTTAc < 1:217733/149‑1 (MQ=37) aTGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc > 7:108124/1‑149 (MQ=255) aTGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc < 3:256467/149‑1 (MQ=255) gggTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTGACCcgc > 4:72417/1‑149 (MQ=255) ggTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc > 1:19112/1‑148 (MQ=255) ggTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcgc < 2:53631/148‑1 (MQ=255) tCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAgg > 3:76213/1‑149 (MQ=255) tCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAgg > 7:283188/1‑149 (MQ=255) tCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg > 2:43117/1‑130 (MQ=38) tCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTAcgggcggg < 1:43117/130‑1 (MQ=38) cAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTGGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg < 6:127852/149‑1 (MQ=255) tGGGCGGTTATCGCGTTTGAAACGCTTGACGGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCATCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑Ac < 7:344504/149‑1 (MQ=38) cGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCa < 4:362282/149‑1 (MQ=255) ggTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCaa < 7:1057/149‑1 (MQ=255) gCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCa < 8:108124/148‑1 (MQ=255) ggAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGAcgc > 3:295543/1‑149 (MQ=255) ggggAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGcaac > 4:236667/1‑148 (MQ=255) gaggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACaaaa < 4:295543/149‑1 (MQ=255) aggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc < 2:359006/83‑1 (MQ=255) aggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc > 1:359006/1‑83 (MQ=255) gaTTTAGCGGTTGTTCGGCGATTTAATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGTATCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAccc < 7:133188/149‑1 (MQ=38) tatcGGTTGTTAAGCGAATTTATTTTTGTCTGTGCTGGCCTCATCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGATGCCAGCACAGGCGATAATACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGc < 1:259023/145‑1 (MQ=38) gCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGc > 5:8408/1‑149 (MQ=255) ggTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg < 4:263586/149‑1 (MQ=255) ggTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg > 1:331290/1‑149 (MQ=255) gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTAACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGt > 7:341564/1‑149 (MQ=255) gcgATTTTTTTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACgg < 5:272261/149‑1 (MQ=255) gcgATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAGCCCGCCCGTAGGAGCGGGTTCACCCGGGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCATCACTACgg > 7:123485/1‑149 (MQ=255) gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCt < 4:76213/149‑1 (MQ=255) gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCt > 3:275197/1‑149 (MQ=255) gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAACAGGCCAGCACAGGCGATAA‑ACATCAAAGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAACCCGACACTACgg > 4:91341/1‑147 (MQ=255) tttattttTGTCTGTGCTGGCCTCATCGGGGGTAAACCCGCCGGTAGGCGCGGGTTGGCCCGGGAAGGGGCCCGCACAGGGGATTA‑ACATGAAGGCGCAGGCCGGGCCGGGACCTGCGAGAAGACCCGCAAGCCGTGACTACgggggt > 6:202680/1‑144 (MQ=255) ttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTc < 2:61298/148‑1 (MQ=255) gccTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg > 4:66503/3‑149 (MQ=25) tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAgg > 1:194964/1‑149 (MQ=255) tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAgg < 2:19112/149‑1 (MQ=255) tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAg < 8:283188/148‑1 (MQ=255) tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc < 4:275197/146‑1 (MQ=255) tGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg > 8:33615/1‑149 (MQ=37) gCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCgg < 3:236667/147‑1 (MQ=255) cgGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTg > 5:305151/1‑148 (MQ=37) cgGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGc < 2:194964/149‑1 (MQ=255) gggTGAACCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCa > 6:55399/1‑149 (MQ=38) gTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCAc > 3:361876/1‑148 (MQ=255) aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc < 5:202680/149‑1 (MQ=255) aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATc > 3:55315/1‑149 (MQ=255) cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc > 6:26445/1‑149 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 3:218211/1‑149 (MQ=255) ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCAGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc > 8:189244/1‑149 (MQ=255) cgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt < 6:8408/149‑1 (MQ=255) ccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGca > 6:194666/1‑149 (MQ=255) | GGCTGGAAGCGGTGGACATGGGTAAATCCTCGCGAGCAAGC‑GGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGGCGATAA‑ACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCA > NC_002947/4796466‑4796760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |