Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,817,897 A→T 5.9% K198I (AAA→ATA) ‡ PP_4242 → conserved protein of unknown function with Rhs repeat‑associated core domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,817,8970AT 5.9% 199.7 / 2.8 68K198I (AAA→ATA) ‡PP_4242conserved protein of unknown function with Rhs repeat‑associated core domain
Reads supporting (aligned to +/- strand):  ref base A (29/35);  new base T (2/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

GGTGGCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTCGAGT  >  NC_002947/4817752‑4818044
                                                                                                                                                 |                                                                                                                                                   
ggtggCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATTGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAg                                                                                                                                                   <  7:151222/148‑1 (MQ=255)
  tggCCTCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGTCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTg                                                                                                                                                <  2:103589/149‑1 (MQ=255)
  tggCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACaa                                                                                                                                                     >  8:226983/1‑144 (MQ=255)
  tggCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACaa                                                                                                                                                     <  7:226983/144‑1 (MQ=255)
  tggCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGt                                                                                                                                                 >  4:275245/1‑148 (MQ=255)
  tggCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGt                                                                                                                                                 >  2:199079/1‑148 (MQ=255)
      aTCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGc                                                                                                                                            >  4:270125/1‑149 (MQ=255)
         agtaaatgcatgttcttcaattaaataactttccctggttgcctcatttttcccatcccatatttttttgatcttgcccgcagccacaagcacatcgaaatccttgatgtacacaaccaaatacaCGAGCTTCACAAAGGTGAAGCTc                                                                                                                                          >  1:238524‑M1/126‑148 (MQ=255)
         aGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTc                                                                                                                                          <  5:272431/148‑1 (MQ=255)
         aGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCg                                                                                                                                         <  8:297683/149‑1 (MQ=255)
         aGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCg                                                                                                                                         >  3:268578/1‑149 (MQ=255)
           gtaaatgcatgttcttcaattaaataactttccctggttgcctcatttttcccagcccatattattttgatcttgcccgcagccacaagcacatcgaaatccttgatgtacacaccaaatacaCGAGCTTCACAAAGGTGAAGCTCgt                                                                                                                                        >  5:187421‑M1/124‑148 (MQ=255)
                  aCGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTgg                                                                                                                                >  4:49941/1‑149 (MQ=255)
                   cGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTgg                                                                                                                                <  3:270125/148‑1 (MQ=255)
                   cGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgt                                                                                                                               <  3:275245/149‑1 (MQ=255)
                   cGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgt                                                                                                                               <  6:59582/149‑1 (MQ=255)
                    gTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtg                                                                                                                              <  2:288882/149‑1 (MQ=255)
                     tGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtgt                                                                                                                             >  7:26464/1‑149 (MQ=255)
                      ggTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtgtg                                                                                                                            >  6:110190/1‑149 (MQ=255)
                       gTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtgtgt                                                                                                                           <  6:99959/149‑1 (MQ=255)
                       gTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtgtg                                                                                                                            >  1:68520/1‑148 (MQ=255)
                       gTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGgtgtg                                                                                                                            <  7:290736/148‑1 (MQ=255)
                            tAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTAc                                                                                                                         <  1:50890/146‑1 (MQ=255)
                             agagTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATc                                                                                                                      <  6:79495/148‑1 (MQ=255)
                               agTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAg                                                                                                                   <  8:320677/149‑1 (MQ=255)
                                 tCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATAGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGa                                                                                                                 >  3:276246/1‑149 (MQ=255)
                                   tGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGAtt                                                                                                               >  5:31408/1‑149 (MQ=255)
                                   tGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGAtt                                                                                                               >  7:338572/1‑149 (MQ=255)
                                       gTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGa                                                                                                           >  6:382648/1‑149 (MQ=255)
                                        ttCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGAt                                                                                                          <  5:110190/149‑1 (MQ=255)
                                        ttCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGAt                                                                                                          <  4:268578/149‑1 (MQ=255)
                                        ttCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGAt                                                                                                          <  6:31408/149‑1 (MQ=255)
                                          cAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGGTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGAtgt                                                                                                        <  8:185039/149‑1 (MQ=255)
                                                     aCATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTGGATGTGCTTGGGGCTg                                                                                             >  6:282208/1‑149 (MQ=255)
                                                     aCATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTg                                                                                             <  1:199079/149‑1 (MQ=255)
                                                          aaaTTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCggg                                                                                         >  3:34003/1‑148 (MQ=255)
                                                           aaTTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGc                                                                                        >  2:8148/1‑148 (MQ=255)
                                                           aaTTGTAGAACAAGGGCACGGCTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAATGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGc                                                                                        >  4:301665/1‑148 (MQ=255)
                                                               gttgATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGa                                                                                    <  3:130369/148‑1 (MQ=255)
                                                               gttgATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGa                                                                                    <  3:49941/148‑1 (MQ=255)
                                                                    tCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCaaa                                                                               >  8:124946/1‑148 (MQ=255)
                                                                       tGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCaaaaaaa                                                                           <  1:22353/149‑1 (MQ=255)
                                                                           cAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATatg                                                                       <  8:26464/149‑1 (MQ=255)
                                                                             ggggTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATatggg                                                                     >  7:294740/1‑149 (MQ=255)
                                                                               ggTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATatgggat                                                                   >  1:45089/1‑149 (MQ=255)
                                                                                    tattttaCACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATAtgggatggga                                                               >  2:81854/1‑148 (MQ=255)
                                                                                            acGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAgg                                                      >  1:371009/1‑149 (MQ=255)
                                                                                             cGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGc                                                     <  1:8148/149‑1 (MQ=255)
                                                                                              cacaagcacatcgaaatccttgatgtacacaccaaatacaCGAGCTTCACCTTTGTGAAGCTTGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGc                                                     <  4:289217‑M1/108‑1 (MQ=255)
                                                                                                aaCTATCGCGTGGCCGAGCCCTATGCGAATGCAGCCGCCAAGCATCACAAAGCTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCGTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAAc                                                  <  6:190951/149‑1 (MQ=255)
                                                                                                   tATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAg                                               >  4:209079/1‑149 (MQ=255)
                                                                                                     tGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAATAATGAGGCAACCAggg                                             >  4:364539/1‑149 (MQ=255)
                                                                                                       gCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGa                                            >  8:182645/1‑148 (MQ=255)
                                                                                                          tGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGt                                        <  4:34003/149‑1 (MQ=255)
                                                                                                              cGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTAtt                                    <  3:209079/149‑1 (MQ=255)
                                                                                                               gAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTAttt                                   <  7:124946/149‑1 (MQ=255)
                                                                                                                cttgatgtacacaccaaatacaCGAGCTTCACCTTTGTGAAGCTTGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTAttt                                   <  6:214684‑M1/126‑1 (MQ=255)
                                                                                                                 ttgatgtacacaccaaatacaCGAGCTTCACCTTTGTGAAGCTTGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTaa                                 >  5:214684‑M1/22‑149 (MQ=255)
                                                                                                                    cTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAAtt                               >  7:129103/1‑148 (MQ=255)
                                                                                                                    cTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTg                              <  8:338572/149‑1 (MQ=255)
                                                                                                                     tgtacacaccaaatacaCGAGCTTCACCTTTGTGAAGCTTGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTga                             >  3:289217‑M1/18‑149 (MQ=255)
                                                                                                                       tGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTgaa                            <  7:182645/148‑1 (MQ=255)
                                                                                                                          gAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCCTGTATTTGGTGTGTAAATAAAGGATTTCGATGTGCTTGTGGCTGCCGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTgaagaa                         >  2:313641/1‑148 (MQ=255)
                                                                                                                                aGCCGCCAAGCTTCACAAAGGTGAAGCTCGAGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCAt                  >  1:30100/1‑149 (MQ=255)
                                                                                                                                      CAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACt             <  4:52421‑M1/148‑1 (MQ=255)
                                                                                                                                      CAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTa            <  7:319914‑M1/149‑1 (MQ=255)
                                                                                                                                        accatCACAACGGTCAAGCTCGTTTATTTGGGGTGTAAATCAACGATATCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAat          <  4:276246/145‑1 (MQ=255)
                                                                                                                                        aGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAat          <  2:371009/149‑1 (MQ=255)
                                                                                                                                             cacaAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTc      <  8:294740/148‑1 (MQ=255)
                                                                                                                                              acaAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTCGa    <  8:129103/149‑1 (MQ=255)
                                                                                                                                                aaaGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTCGAg   >  8:93468/1‑148 (MQ=255)
                                                                                                                                                aaaGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATCGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTCGAGt  <  2:30100/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGTGGCATCAGGAGCTCTACGTGGTCTTTAGAGTCTGGCGTTCAGCCCCAGCGACATCAAATTGTTGATCATGGGCAGGGGTTTTATTTTACACGCAACTATGGCGTGGCCGAGCGCTATGCGAATGCAGCCGCCAAGCTTCACAAAGGTGAAGCTCGTGTATTTGGTGTGTACATCAAGGATTTCGATGTGCTTGTGGCTGCGGGCAAGATCAAAAAAATATGGGATGGGAAAAATGAGGCAACCAGGGAAAGTTATTTAATTGAAGAACATGCATTTACTAATATTCGAGT  >  NC_002947/4817752‑4818044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑