Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,902,585	C→T	6.2%	A167V (GCC→GTC)	PP_4310 →	hydantoin racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,902,585	0	C	T	6.2%	194.8 / 2.1	64	A167V (GCC→GTC)	PP_4310	hydantoin racemase
Reads supporting (aligned to +/- strand):  ref base C (34/26);  new base T (2/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGG  >  NC_002947/4902438‑4902731                                                                                                                                                    |                                                                                                                                                   gCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGcc                                                                                                                                                   >  6:221742/1‑149 (MQ=255)         ccATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGcga                                                                                                                                           <  2:261357/149‑1 (MQ=255)          cATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGcgac                                                                                                                                          <  3:29712/149‑1 (MQ=255)           aTTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGcgac                                                                                                                                          >  3:152714/1‑148 (MQ=255)              gATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCgacgac                                                                                                                                       >  8:36907/1‑148 (MQ=255)                      aCCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAg                                                                                                                              <  5:221742/149‑1 (MQ=255)                          ccTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGa                                                                                                                          <  7:36907/149‑1 (MQ=255)                            tGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATc                                                                                                                        >  3:167501/1‑149 (MQ=255)                                 ctctCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCt                                                                                                                   <  5:163615/149‑1 (MQ=255)                                   ctcGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTgg                                                                                                                 <  3:54783/149‑1 (MQ=255)                                    tcGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTggg                                                                                                                >  5:3303/1‑149 (MQ=255)                                    tcGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTggg                                                                                                                >  3:292562/1‑149 (MQ=255)                                        gCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTtgt                                                                                                            >  6:375251/1‑149 (MQ=255)                                          cTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTgg                                                                                                          >  7:76281/1‑149 (MQ=255)                                          cTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTgg                                                                                                          >  3:129870/1‑149 (MQ=255)                                            gTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTgg                                                                                                                 >  8:210319/1‑140 (MQ=255)                                            gTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTgg                                                                                                                 <  7:210319/140‑1 (MQ=255)                                             tATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTggcgg                                                                                                       <  6:3303/149‑1 (MQ=255)                                             tATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTggcgg                                                                                                       <  2:104955/149‑1 (MQ=255)                                             tATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTggcgg                                                                                                       >  3:335288/1‑149 (MQ=255)                                                 aCCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGTCATg                                                                                                   <  6:123647/149‑1 (MQ=255)                                                   cGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGc                                                                                                 <  4:152714/149‑1 (MQ=255)                                                        cgcCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCggg                                                                                             <  1:287129/148‑1 (MQ=255)                                                        cgcCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGc                                                                                            <  5:375251/149‑1 (MQ=255)                                                         gcCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCt                                                                                           <  4:335288/149‑1 (MQ=255)                                                          ccgcGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTg                                                                                          <  7:255845/146‑1 (MQ=255)                                                           cTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTgg                                                                                         <  2:34302/149‑1 (MQ=255)                                                           cTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGGTGTGGCGGCATGGCCGGGCTg                                                                                          >  6:298670/1‑148 (MQ=255)                                                            tCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGa                                                                                        >  6:103639/1‑149 (MQ=255)                                                             cGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCt                                                                                                                   <  1:201016/121‑1 (MQ=255)                                                             cGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCt                                                                                                                   >  2:201016/1‑121 (MQ=255)                                                                tGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGACGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGAGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTTGACGAg                                                                                    <  2:296287/149‑1 (MQ=255)                                                                   gAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGTCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCgg                                                                                              >  3:234407/1‑136 (MQ=255)                                                                   gAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGTCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCgg                                                                                              <  2:117410/136‑1 (MQ=255)                                                                   gAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGTCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCgg                                                                                              <  4:234407/136‑1 (MQ=255)                                                                   gAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGTCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCgg                                                                                              >  1:117410/1‑136 (MQ=255)                                                                    cgCCAGCCCGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAg                                                                                    >  2:142244/2‑145 (MQ=255)                                                                                        tggaactggaaGCCGACCCGCTACGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTg                                                            >  7:234696/1‑149 (MQ=255)                                                                                          gaactggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGcc                                                          >  3:271496/1‑149 (MQ=255)                                                                                              tggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtg                                                      >  6:295145/1‑149 (MQ=255)                                                                                              tggaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtg                                                      >  5:119217/1‑149 (MQ=255)                                                                                                gcaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtggt                                                    <  6:89900/147‑1 (MQ=255)                                                                                                gaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtggt                                                    >  7:292561/1‑149 (MQ=255)                                                                                                gaaGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCggtg                                                      <  5:295145/147‑1 (MQ=255)                                                                                                  aGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGtggtgg                                                  <  4:129870/149‑1 (MQ=255)                                                                                                      gACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATgg                                              >  7:285937/1‑149 (MQ=255)                                                                                                      gACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATgg                                              >  2:93309/1‑149 (MQ=255)                                                                                                        cccGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGgtg                                            >  6:27346/1‑149 (MQ=255)                                                                                                          cGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGc                                                           <  7:115057/132‑1 (MQ=255)                                                                                                          cGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGc                                                           >  8:115057/1‑132 (MQ=255)                                                                                                           gCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGa                                         <  1:142244/149‑1 (MQ=255)                                                                                                                 ggCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAg                                   >  3:40863/1‑149 (MQ=255)                                                                                                                  gCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGc                                  <  4:271496/149‑1 (MQ=255)                                                                                                                        gAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGAc                            <  4:167501/149‑1 (MQ=255)                                                                                                                             gATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTg                       >  5:384545/1‑149 (MQ=255)                                                                                                                                   cGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCACCAACGCACCGGCGTGCCGGTGGTGGAt                                                >  1:291799/1‑118 (MQ=255)                                                                                                                                   cGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCACCAACGCACCGGCGTGCCGGTGGTGGAt                                                <  2:291799/118‑1 (MQ=255)                                                                                                                                    gAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATc                >  7:323453/1‑149 (MQ=255)                                                                                                                                     aGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCg               >  2:358048/1‑149 (MQ=255)                                                                                                                                          gCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGt          >  6:149945/1‑149 (MQ=255)                                                                                                                                          gCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGt          >  7:353056/1‑149 (MQ=255)                                                                                                                                           ccGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGTCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTg         >  6:199781/1‑149 (MQ=255)                                                                                                                                              aaCGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTgcg       >  1:189878/1‑148 (MQ=255)                                                                                                                                              aaCGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTgcg       >  1:265349/1‑148 (MQ=255)                                                                                                                                                cgcgCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCAt    >  1:189759/1‑149 (MQ=255)                                                                                                                                                 gcgcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATg   >  6:243510/1‑149 (MQ=255)                                                                                                                                                  cgcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATgg  <  4:292562/149‑1 (MQ=255)                                                                                                                                                  cgcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATgg  <  5:298670/149‑1 (MQ=255)                                                                                                                                                  cgcCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATgg  <  8:292561/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCACCGTGCCATTGATCGAGGACCGCCTGAAGCTCTCGGGCCTGTATGACCGCTGCGCCTCGGTGCGAGCCAGCGGGCTGGCGGTGCTGGAACTGGAAGCCGACCCGCTGCGGGCCGTGGAGGCGATTGTCGAGCAGGCCGAACGCGCCGTGCGCGACGACAAGGCCGAGGTGATCTGCCTGGGTTGTGGCGGCATGGCCGGGCTGGACGAGCAGATTCGCCAACGCACCGGCGTGCCGGTGGTGGATGGTGTGAGTGCAGCGGTGACCATTGCCGAATCGCTGGTGCGCATGG  >  NC_002947/4902438‑4902731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑