Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,906,463	A→T	8.6%	L112Q (CTG→CAG)	PP_4315 ←	epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,906,463	0	A	T	8.6%	162.7 / 4.8	58	L112Q (CTG→CAG)	PP_4315	epimerase
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base T (2/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

AACGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGG  >  NC_002947/4906316‑4906610                                                                                                                                                     |                                                                                                                                                    aaCGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCca                                                                                                                                                     <  8:242005/148‑1 (MQ=255)  aCGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCcag                                                                                                                                                    >  5:310836/1‑148 (MQ=255)   cGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCcag                                                                                                                                                    >  2:37807/1‑147 (MQ=255)    gTTTCGATCAGCATGTCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCctgctg                                                                                                                                                 <  6:4877/149‑3 (MQ=255)    gTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCcagcag                                                                                                                                                 <  6:128404/149‑1 (MQ=255)         gAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAt                                                                                                                                            <  2:302456/149‑1 (MQ=255)          agcagcATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           <  6:66071/149‑2 (MQ=255)           gcagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           >  6:66445/1‑147 (MQ=255)           gcagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           <  5:66445/148‑2 (MQ=255)                     gTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGc                                                                                                                                >  7:212240/1‑149 (MQ=255)                      tCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCTCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                               <  8:163690/149‑1 (MQ=255)                      tCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                               <  8:212240/149‑1 (MQ=255)                         tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCca                                                                                                                                                     <  8:31491/124‑1 (MQ=255)                         tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCca                                                                                                                                                     >  7:31491/1‑124 (MQ=255)                         tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                            <  2:154493/149‑1 (MQ=255)                         tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                            <  6:230116/149‑1 (MQ=255)                            aTCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgcg                                                                                                                         >  5:337017/1‑149 (MQ=255)                                 cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACt                                                                                                                    >  5:282254/1‑149 (MQ=255)                                  acGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCCGCGCCTGCAAACGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTc                                                                                                                   <  8:335976/149‑1 (MQ=255)                                   cGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCg                                                                                                                  <  1:37807/149‑1 (MQ=255)                                      cATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCTCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCa                                                                                                               <  7:51356/149‑1 (MQ=255)                                         aaTCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACt                                                                                                             >  6:202085/1‑148 (MQ=255)                                           tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGc                                                                                                                 <  5:337205/142‑1 (MQ=255)                                           tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGc                                                                                                                 >  6:337205/1‑142 (MQ=255)                                             gtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCgg                                                                                                         <  1:374755/148‑1 (MQ=255)                                                  tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCAACAGCGCAACGGCTTACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    >  8:163605/1‑148 (MQ=255)                                                     ggTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaag                                                                                                <  6:381566/149‑1 (MQ=255)                                                          acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           >  4:232057/1‑149 (MQ=255)                                                          acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           <  2:352544/149‑1 (MQ=255)                                                           ccGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           >  6:178086/1‑148 (MQ=255)                                                                   gCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  <  6:310836/149‑1 (MQ=255)                                                                     cTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCt                                                                                >  8:114058/1‑149 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAACAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTAGGCGGGGAAGTCCATGGCCAGCAGGCCCTCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGCAGCGAAGCACGTGCGCCGGCGCa                                                                           >  7:346523/1‑149 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaa                                                                                            <  5:317229/132‑1 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaa                                                                                            >  6:317229/1‑132 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  4:359848/1‑149 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  6:167687/1‑149 (MQ=255)                                                                          tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  2:279861/1‑149 (MQ=255)                                                                            aaGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAcc                                                                         >  1:291315/1‑149 (MQ=255)                                                                            aaGCCCAGCGCCTGCAAAAGGCCCGCGGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                          <  8:87529/148‑1 (MQ=255)                                                                               cccAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAg                                                                      <  6:337017/149‑1 (MQ=255)                                                                                     gcCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                <  8:967/149‑1 (MQ=255)                                                                                     gcCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                <  5:167687/149‑1 (MQ=255)                                                                                         gCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCAc                                                                                        <  1:255335/121‑1 (MQ=255)                                                                                         gCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCAc                                                                                        >  2:255335/1‑121 (MQ=255)                                                                                          cAAAAGGCCGGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGTGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAAc                                                            <  5:202085/148‑1 (MQ=255)                                                                                          cAAAAGGCCCGCTGGCATGTCGACCGCTGACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGaa                                                             >  1:22923/1‑148 (MQ=255)                                                                                                  ccGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGt                                                     >  2:166227/1‑147 (MQ=255)                                                                                                  ccGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTaa                                                   <  2:26521/149‑1 (MQ=255)                                                                                                        gCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGa                                              <  3:232057/148‑1 (MQ=255)                                                                                                               gACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAgg                                      <  6:178633/149‑1 (MQ=255)                                                                                                                        cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  >  5:90796/1‑97 (MQ=255)                                                                                                                        cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  <  6:90796/97‑1 (MQ=255)                                                                                                                         gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    >  4:122917/1‑78 (MQ=255)                                                                                                                         gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    <  3:122917/78‑1 (MQ=255)                                                                                                                         gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGc                             >  4:367350/1‑149 (MQ=255)                                                                                                                                  ggCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAgg                     <  5:178086/148‑1 (MQ=255)                                                                                                                                   gCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGt                    <  2:22923/148‑1 (MQ=255)                                                                                                                                       ggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACt               >  5:32230/1‑149 (MQ=255)                                                                                                                                             tgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCTGCACAGGTCGACTTCCACg         >  5:203469/3‑149 (MQ=255)                                                                                                                                             ccATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCTAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg         >  6:198174/1‑149 (MQ=255)                                                                                                                                              cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACgg        >  7:181844/1‑149 (MQ=255)                                                                                                                                              cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACgg        >  6:217547/1‑149 (MQ=255)                                                                                                                                               aTGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGt       <  6:282254/149‑1 (MQ=255)                                                                                                                                                   ccAGCAGGGCATCAGGGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGGGAAGCACTTGCGCGTGGTCACCTTTTTGCTGGAACAGCACGTAAGCCGAGGCCTAGGTAGCATGGCGGCACAGGTCGACTTCCACGGTgggg   <  1:304022/149‑1 (MQ=255)                                                                                                                                                    cagcagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTggggg  <  7:163605/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACGTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGG  >  NC_002947/4906316‑4906610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑