Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,906,466	A→T	8.2%	L111Q (CTG→CAG)	PP_4315 ←	epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,906,466	0	A	T	8.2%	160.5 / 3.5	58	L111Q (CTG→CAG)	PP_4315	epimerase
Reads supporting (aligned to +/- strand):  ref base A (25/28);  new base T (2/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGG  >  NC_002947/4906319‑4906610                                                                                                                                                     |                                                                                                                                                 gTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCcagcag                                                                                                                                                 <  6:128404/149‑1 (MQ=255)      gAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAt                                                                                                                                            <  2:302456/149‑1 (MQ=255)       agcagcATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           <  6:66071/149‑2 (MQ=255)        gcagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           <  5:66445/148‑2 (MQ=255)        gcagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtg                                                                                                                                           >  6:66445/1‑147 (MQ=255)                  gTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGc                                                                                                                                >  7:212240/1‑149 (MQ=255)                   tCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCTCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                               <  8:163690/149‑1 (MQ=255)                   tCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCt                                                                                                                               <  8:212240/149‑1 (MQ=255)                      tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                            <  6:230116/149‑1 (MQ=255)                      tCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAc                                                                                                                            <  2:154493/149‑1 (MQ=255)                         aTCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGAcgcg                                                                                                                         >  5:337017/1‑149 (MQ=255)                              cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACt                                                                                                                    >  5:282254/1‑149 (MQ=255)                               acGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCCGCGCCTGCAAACGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTc                                                                                                                   <  8:335976/149‑1 (MQ=255)                                cGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCg                                                                                                                  <  1:37807/149‑1 (MQ=255)                                   cATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCTCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCa                                                                                                               <  7:51356/149‑1 (MQ=255)                                      aaTCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACt                                                                                                             >  6:202085/1‑148 (MQ=255)                                        tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGc                                                                                                                 <  5:337205/142‑1 (MQ=255)                                        tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGc                                                                                                                 >  6:337205/1‑142 (MQ=255)                                          gtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCgg                                                                                                         <  1:374755/148‑1 (MQ=255)                                               tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCAACAGCGCAACGGCTTACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    >  8:163605/1‑148 (MQ=255)                                                  ggTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaag                                                                                                <  6:381566/149‑1 (MQ=255)                                                       acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           >  4:232057/1‑149 (MQ=255)                                                       acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           <  2:352544/149‑1 (MQ=255)                                                        ccGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                           >  6:178086/1‑148 (MQ=255)                                                                gCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  <  6:310836/149‑1 (MQ=255)                                                                  cTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCt                                                                                >  8:114058/1‑149 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAACAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTAGGCGGGGAAGTCCATGGCCAGCAGGCCCTCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGCAGCGAAGCACGTGCGCCGGCGCa                                                                           >  7:346523/1‑149 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaa                                                                                            <  5:317229/132‑1 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaa                                                                                            >  6:317229/1‑132 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  2:279861/1‑149 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  4:359848/1‑149 (MQ=255)                                                                       tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCa                                                                           >  6:167687/1‑149 (MQ=255)                                                                         aaGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAcc                                                                         >  1:291315/1‑149 (MQ=255)                                                                         aaGCCCAGCGCCTGCAAAAGGCCCGCGGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                          <  8:87529/148‑1 (MQ=255)                                                                            cccAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAg                                                                      <  6:337017/149‑1 (MQ=255)                                                                                  gcCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                <  8:967/149‑1 (MQ=255)                                                                                  gcCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                <  5:167687/149‑1 (MQ=255)                                                                                      gCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCAc                                                                                        <  1:255335/121‑1 (MQ=255)                                                                                      gCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCAc                                                                                        >  2:255335/1‑121 (MQ=255)                                                                                       cAAAAGGCCGGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGTGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAAc                                                            <  5:202085/148‑1 (MQ=255)                                                                                       cAAAAGGCCCGCTGGCATGTCGACCGCTGACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGaa                                                             >  1:22923/1‑148 (MQ=255)                                                                                               ccGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGt                                                     >  2:166227/1‑147 (MQ=255)                                                                                               ccGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTaa                                                   <  2:26521/149‑1 (MQ=255)                                                                                                     gCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGa                                              <  3:232057/148‑1 (MQ=255)                                                                                                            gACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAgg                                      <  6:178633/149‑1 (MQ=255)                                                                                                                     cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  >  5:90796/1‑97 (MQ=255)                                                                                                                     cGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                  <  6:90796/97‑1 (MQ=255)                                                                                                                      gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    >  4:122917/1‑78 (MQ=255)                                                                                                                      gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                    <  3:122917/78‑1 (MQ=255)                                                                                                                      gggTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGc                             >  4:367350/1‑149 (MQ=255)                                                                                                                               ggCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAgg                     <  5:178086/148‑1 (MQ=255)                                                                                                                                gCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGt                    <  2:22923/148‑1 (MQ=255)                                                                                                                                    ggAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACt               >  5:32230/1‑149 (MQ=255)                                                                                                                                          tgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCTGCACAGGTCGACTTCCACg         >  5:203469/3‑149 (MQ=255)                                                                                                                                          ccATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCTAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg         >  6:198174/1‑149 (MQ=255)                                                                                                                                           cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACgg        >  7:181844/1‑149 (MQ=255)                                                                                                                                           cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACgg        >  6:217547/1‑149 (MQ=255)                                                                                                                                            aTGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGt       <  6:282254/149‑1 (MQ=255)                                                                                                                                                ccAGCAGGGCATCAGGGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGGGAAGCACTTGCGCGTGGTCACCTTTTTGCTGGAACAGCACGTAAGCCGAGGCCTAGGTAGCATGGCGGCACAGGTCGACTTCCACGGTgggg   <  1:304022/149‑1 (MQ=255)                                                                                                                                                 cagcagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTggggg  <  7:163605/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GTTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCT‑ACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGG  >  NC_002947/4906319‑4906610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑