| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,944,743 | A→G | 6.5% | intergenic (+103/+695) | PP_5673 → / ← flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,944,743 | 0 | A | G | 6.5% | 273.2 / 6.2 | 92 | intergenic (+103/+695) | PP_5673/flhB | D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein |
| Reads supporting (aligned to +/- strand): ref base A (39/47); new base G (3/3); total (42/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
TTATCTTAAACAACTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGACCAGC > NC_002947/4944603‑4944881 | ttATCTTAAACAACTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTc > 1:337950/1‑149 (MQ=255) aacaacTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGTATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGa < 6:41414/149‑1 (MQ=255) aacaacTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTg < 1:110854/148‑1 (MQ=255) aacaacTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGa < 6:184462/149‑1 (MQ=255) aacaacTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGa < 3:117770/149‑1 (MQ=255) caacTTCGATCCTACCCAATACTGCTGACCGAACAACAACTCTAATTCCTACCATCAGGCT‑ATTGGATGTCGTAGCGGTTTTTCGGATTGTAATTTATTGATCACCCAATTGAGATTAACCGCCGGTTGAGCGGCGCTTAATCtcaatt < 8:288802/149‑6 (MQ=255) aacTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCTGATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAg > 1:294235/1‑148 (MQ=255) aTCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAg < 1:25163/149‑1 (MQ=255) tAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAAc > 4:44459/1‑149 (MQ=255) aGCCCATACTGCTGACCCAAGAACAAGTATAATTCGTACCATCAGGCT‑AATGGATGTCGTTCGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACg < 8:241954/149‑1 (MQ=255) gCCAATACTTCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGATATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAAc < 2:313113/147‑1 (MQ=255) gCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGTTTGAGCGGCGCttaa < 4:153897/127‑4 (MQ=255) gCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGTTTGAGCGGCGCttaa > 3:153897/1‑124 (MQ=255) gCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACgg > 8:221236/1‑149 (MQ=255) gCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACAGCGCTCGATCTTGAGCAATCCCAGAAACgg < 4:79678/149‑1 (MQ=255) ccAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGt < 6:314858/149‑1 (MQ=255) aTACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGTTTGAGCGGCGCTTAATCtcaa < 2:178176/129‑4 (MQ=255) aTACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGTTTGAGCGGCGCTTAATCtcaa > 1:178176/1‑126 (MQ=255) aCTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATTGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGa < 3:280934/149‑1 (MQ=255) tgctgACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGaa > 1:48930/1‑148 (MQ=255) aCCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAg > 6:304321/1‑121 (MQ=255) aCCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACttt < 8:114034/148‑1 (MQ=255) ccGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTc > 1:203410/1‑148 (MQ=255) cGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTc < 6:110979/136‑1 (MQ=255) cGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTc > 5:110979/1‑136 (MQ=255) aacaaGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGt > 7:116370/1‑148 (MQ=255) acaaGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCa < 3:225703/149‑1 (MQ=255) aaGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCAcg > 3:332136/1‑149 (MQ=255) aaGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCAcg > 5:198442/1‑149 (MQ=255) gTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCAcgcc < 2:203410/149‑1 (MQ=255) aTTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAAc < 2:361151/128‑1 (MQ=255) aTTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAAc > 1:361151/1‑128 (MQ=255) ttCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTg > 2:250220/1‑101 (MQ=255) ttCGTACAATCATGCT‑ATTGGATGTGGTTGCTGTTTTTCGCATGTTAATTTTTTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAACCGGTCAAGAAACTTTCAACTGTCACGCCGCAAAt < 2:360909/149‑1 (MQ=255) tCGTACAATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGAGCTCGATCTTGAGCAATCACAGAAACGGTCAAGAAACTTTCAAATTTCAAGCCGCAAATc > 5:25087/1‑149 (MQ=255) tACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCcgg < 6:361572/149‑1 (MQ=255) tACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCACTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCcgg > 5:145672/1‑149 (MQ=255) gaGGCT‑TTTTGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTTATCACACAATTGAGATTAAGCGCATCTCAAACGGCGCTGGATCTTGAGCAATCCCAGAAACGTTCAAGAAACTTTCTACTGTCAAGCCGCAAATCCGGGGGaa < 2:48930/147‑1 (MQ=255) cAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAAt > 3:353380/1‑149 (MQ=255) aGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATc < 2:337950/149‑1 (MQ=255) ggCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCg > 6:328352/1‑149 (MQ=255) cT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGa < 5:328352/149‑1 (MQ=255) t‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGa > 3:133119/1‑148 (MQ=255) aTTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGa > 5:217049/1‑147 (MQ=255) ttGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAg > 2:14258/1‑149 (MQ=255) ggATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGc < 7:179594/148‑1 (MQ=255) gATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGa > 7:130314/1‑82 (MQ=255) gATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGa < 8:130314/82‑1 (MQ=255) gATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAg > 3:328318/1‑149 (MQ=255) cGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATgg > 8:157352/1‑149 (MQ=255) ttGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTc < 2:55805/149‑1 (MQ=255) tGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGAAAAGCAGGATGGTCg > 1:301692/1‑149 (MQ=255) gtgtTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCggg < 8:116370/149‑1 (MQ=255) gtgtTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCgg < 4:133119/148‑1 (MQ=255) gtgtTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCgg < 4:353380/148‑1 (MQ=255) gtgtTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCgg > 8:29186/1‑148 (MQ=255) gtgtTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCgg < 1:146680/148‑1 (MQ=255) ggATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGc > 3:86417/1‑148 (MQ=255) ggATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGc > 3:91622/1‑148 (MQ=255) gTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACt < 1:301398/91‑1 (MQ=255) gTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACt > 2:301398/1‑91 (MQ=255) tAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTc > 1:374135/1‑148 (MQ=255) aTTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGa < 4:86417/148‑1 (MQ=255) aTTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGa < 4:91622/148‑1 (MQ=255) tttATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGAAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGTATGGTCGGGAACGCGGCAAAGACGAGc > 7:334499/1‑149 (MQ=255) ttGATCACCCAAGTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGgc < 8:295777/149‑1 (MQ=255) aTCACCCTATTGATATTAAGCGCCGCTCTAACGGCGCTCGATCTTGATCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACTCCTCAAATCCGGCGGAATCGGAAAGCAGGATGGGCGGGAACGCGGCAAAGGCGAGCGGgcgc < 8:334499/148‑1 (MQ=255) tCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCaa < 7:29186/149‑1 (MQ=255) cACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAg > 1:285318/1‑149 (MQ=255) cccAATTGAGATTAAGCGCCTCTCAAACGGCGCTCGATCTTGAGCAATCCCATAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGTAACGCGGCAAAGTCGAGCGGGCGCAAGGc < 6:25087/149‑1 (MQ=255) ccAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCaaa < 3:251077/130‑1 (MQ=255) ccAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCaaa > 8:288916/1‑130 (MQ=255) ccAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCaaa > 4:251077/1‑130 (MQ=255) ccAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCaaa < 7:288916/130‑1 (MQ=255) aTTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGta > 7:300086/1‑148 (MQ=255) aTTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGAAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCCGCAAAGTCGAGCGGGCGCAAGGCGta > 4:343220/1‑148 (MQ=255) ttGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGtata > 6:30045/1‑149 (MQ=255) aagaTCAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATaca > 2:314111/2‑149 (MQ=255) ccaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGGCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATaca < 8:59262/146‑1 (MQ=255) agaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATacac < 7:221236/149‑1 (MQ=255) agaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATaca < 2:285318/148‑1 (MQ=255) gatcgaGCGCCGTTTGAGCGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATaca > 5:138500/6‑147 (MQ=38) gaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATc < 7:275738/82‑1 (MQ=255) gaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATc > 8:275738/1‑82 (MQ=255) gaTTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATAcaca < 4:328318/149‑1 (MQ=255) aaGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGAc < 4:332136/148‑1 (MQ=255) aaGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGAc < 2:374135/148‑1 (MQ=255) gcgcCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGAcc > 4:240790/1‑147 (MQ=255) gcgcCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGAcc < 3:240790/147‑1 (MQ=255) gcgcCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGACcag < 1:229267/149‑1 (MQ=255) cgccgcTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGACcagc < 6:198442/149‑1 (MQ=255) cgccgcTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGACcagc < 5:155893/149‑1 (MQ=255) | TTATCTTAAACAACTTGGATCCTAGCCAATACTGCTGACCGAACAACAAGTATAATTCGTACCATCAGGCT‑ATTGGATGTCGTTGGTGTTTTTCGGATGGTAATTTATTGATCACCCAATTGAGATTAAGCGCCGCTCAAACGGCGCTCGATCTTGAGCAATCCCAGAAACGGTCAAGAAACTTTCAACTGTCACGCCGCAAATCCGGCGGAATCGGAAAGCAGGATGGTCGGGAACGCGGCAAAGTCGAGCGGGCGCAAGGCGTATACACAGACCAGC > NC_002947/4944603‑4944881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |