Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,948,658	T→C	5.2%	T64A (ACC→GCC)	fliO ←	flagellar protein FliO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,948,658	0	T	C	5.2%	227.5 / 2.2	76	T64A (ACC→GCC)	fliO	flagellar protein FliO
Reads supporting (aligned to +/- strand):  ref base T (41/31);  new base C (2/2);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

CGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCG  >  NC_002947/4948511‑4948806                                                                                                                                                     |                                                                                                                                                       cGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTg                                                                                                                                                       >  2:351133/1‑149 (MQ=255)    ccAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctc                                                                                                                                                    <  4:124350/149‑1 (MQ=255)     cAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCATGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                                                   >  7:244039/1‑149 (MQ=255)     cAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGctct                                                                                                                                                   <  5:226927/149‑1 (MQ=255)       gTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCTGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                  >  6:16151/1‑148 (MQ=255)            tGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcg                                                                                                                                           >  5:128511/1‑149 (MQ=255)             gCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                          <  4:165402/149‑1 (MQ=255)             gCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                          >  1:347870/1‑149 (MQ=255)              cAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑gcgc                                                                                                                                          <  2:347870/148‑1 (MQ=255)                    cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                  <  3:343984/135‑1 (MQ=255)                    cTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTg                                                                                                                                                  >  4:343984/1‑135 (MQ=255)                       gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑Gcagcag                                                                                                                                 <  7:210191/147‑1 (MQ=255)                       gATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcc                                                                                                                               >  4:93600/1‑149 (MQ=255)                        ctGGTGCCGGGGGTATGGCCGATCAGGCTCTGCTCCTTGCCCCCCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCGCTGCGGCGC‑CTGCAGAGCACC‑gcagtgaagg                                                                                                                              <  5:211844/148‑7 (MQ=255)                          ggTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccagg                                                                                                                            >  2:261645/1‑149 (MQ=255)                           gTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggc                                                                                                                           <  6:10125/149‑1 (MQ=255)                             gCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCGTGTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggc                                                                                                                           >  1:289871/1‑148 (MQ=255)                                gggggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCagaa                                                                                                                      <  6:273787/149‑1 (MQ=255)                                 ggggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCaga                                                                                                                       >  2:211289/1‑147 (MQ=255)                                 ggggTATGGCCGATCAGGATCTGATCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCTGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCaga                                                                                                                       >  8:92808/1‑147 (MQ=255)                                  gggTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCagaaag                                                                                                                    <  3:313725/149‑1 (MQ=255)                                     tATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAaagaag                                                                                                                 <  6:10268/149‑1 (MQ=255)                                          ccGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGAGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGAC‑GCAGCAGCCAGGCCAGAAAGAAGATCAg                                                                                                            >  1:268836/1‑149 (MQ=255)                                           cGATCAGGATCTGCTCCTTGCCCAACTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAgg                                                                                                           <  8:244039/149‑1 (MQ=255)                                             aTCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggcca                                                                                                                         >  6:106242/1‑133 (MQ=255)                                             aTCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgccaggcca                                                                                                                         <  5:106242/133‑1 (MQ=255)                                                 ggATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCccacc                                                                                                     >  5:22943/1‑149 (MQ=255)                                                           cTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                            <  1:351133/148‑1 (MQ=255)                                                            ttGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcca                                                                                                                              >  3:22827/1‑113 (MQ=255)                                                            ttGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAgcca                                                                                                                              <  4:22827/113‑1 (MQ=255)                                                               cccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                       <  5:49921/149‑1 (MQ=255)                                                                ccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                       >  7:29723/1‑148 (MQ=255)                                                                ccACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGcc                                                                                       >  6:140488/1‑148 (MQ=255)                                                                 cACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGccc                                                                                      >  4:257364/1‑148 (MQ=255)                                                                   ccTGCACCAGCAGCAGGCG‑GTCACGCGGTCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcag                                                                                                <  4:126623/136‑1 (MQ=255)                                                                   ccTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAg                                                                                   >  3:259287/1‑149 (MQ=255)                                                                   ccTGCACCAGCAGCAGGCG‑GTCACGCGGACCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcag                                                                                                >  3:126623/1‑136 (MQ=255)                                                                    cTGCACCAGCAGCAGGCG‑GTCACGCGGGCCTATGGCACGGCTGCAGACAATCTCGATCACCTGCCCACCCTTCACTGCTGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGTCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGa                                                                                  <  2:268836/149‑1 (MQ=255)                                                                     tGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGAc                                                                                 >  5:368838/1‑149 (MQ=255)                                                                      gCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGAc                                                                                 >  3:80356/1‑148 (MQ=255)                                                                       cACCAGCAGCAGGCG‑GTCACGCGGGCTGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCa                                                                               <  5:16151/149‑1 (MQ=255)                                                                        aCCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCAt                                                                              >  8:215018/1‑149 (MQ=255)                                                                          cagcagcagGCG‑GTCACGCGGGCCGATGGCACGGCTTCCGACAATCTCGATCACCTGCCCACCATTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCCGCAGCAGGCCCAAGACCATCt                                                                            >  5:19694/1‑149 (MQ=255)                                                                           agcagcagGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTg                                                                           >  2:295706/1‑149 (MQ=255)                                                                                 agGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAg                                                                     >  5:342616/1‑149 (MQ=255)                                                                                  ggCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCccac                                                                                                      <  8:87081/115‑1 (MQ=255)                                                                                        tCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGccc                                                               >  6:77108/1‑149 (MQ=255)                                                                                            gcgGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                            <  4:298646/116‑1 (MQ=255)                                                                                            gcgGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACcagcagc                                                                                            >  3:298646/1‑116 (MQ=255)                                                                                                        cACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGt                                               <  4:259287/149‑1 (MQ=255)                                                                                                           ggCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGa                                            <  6:368838/149‑1 (MQ=255)                                                                                                             cTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCACGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAgc                                          <  2:289871/149‑1 (MQ=255)                                                                                                                     aaTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCgggg                                  >  6:367389/1‑149 (MQ=255)                                                                                                                      aTCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGt                                 <  1:295706/149‑1 (MQ=255)                                                                                                                         tcGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc                              >  3:363912/1‑149 (MQ=255)                                                                                                                         tcGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgc                              >  5:275605/1‑149 (MQ=255)                                                                                                                             tCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGTCCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGgctgct                          <  4:246497/149‑1 (MQ=255)                                                                                                                              cACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGACAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGctgctg                         <  8:121156/149‑1 (MQ=255)                                                                                                                                ccTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGc                       >  2:315992/1‑149 (MQ=255)                                                                                                                                  tGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCCTGCAGCAGCCAGGCTAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCg                      >  1:239105/1‑149 (MQ=255)                                                                                                                                    cccACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTgg                   >  2:372963/1‑149 (MQ=255)                                                                                                                                      cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                 >  4:128756/1‑149 (MQ=255)                                                                                                                                      cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                 >  6:186034/1‑149 (MQ=255)                                                                                                                                      cACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGcc                 >  1:39997/1‑149 (MQ=255)                                                                                                                                       aCCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCg                <  3:128756/149‑1 (MQ=255)                                                                                                                                          cTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAg             <  1:52184/149‑1 (MQ=255)                                                                                                                                             cACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCca          >  7:266119/1‑149 (MQ=255)                                                                                                                                              gcTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTg                    <  6:52108/137‑1 (MQ=255)                                                                                                                                              gcTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTg                    >  5:52108/2‑138 (MQ=255)                                                                                                                                              aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag         <  6:22943/149‑1 (MQ=255)                                                                                                                                              aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag         >  6:187968/1‑149 (MQ=255)                                                                                                                                              aCTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcag         <  5:339880/149‑1 (MQ=255)                                                                                                                                               cTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcagg        >  4:202913/1‑149 (MQ=255)                                                                                                                                               cTGCGGCGC‑CTGCAGAGCACC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcagg        >  6:345352/1‑149 (MQ=255)                                                                                                                                                tGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCcaggc       >  3:225482/1‑149 (MQ=255)                                                                                                                                                    gTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCaggcaggc   <  6:342616/149‑1 (MQ=255)                                                                                                                                                     tGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCg  >  4:352097/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                       CGGCCAGTACGTGCAGGGCCTCGATGGTGCCGGGGGTATGGCCGATCAGGATCTGCTCCTTGCCCACCTGCACCAGCAGCAGGCG‑GTCACGCGGGCCGATGGCACGGCTGCCGACAATCTCGATCACCTGCCCACCCTTCACTGCGGTGCTCTGCAG‑GCGCC‑GCAGCAGCCAGGCCAGAAAGAAGATCAGGCCCACCACCAGCAGCAGGCCCAAGACCATCTGCGCCAGCTGCCCGCCAAGACTGCCAGGTTGAGCCGCCGGGGTGGCTGCTGGCGTGGCCGCAGCCAGGCAGGCG  >  NC_002947/4948511‑4948806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑