Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,995,523	G→C	7.6%	R396P (CGC→CCC) ‡	bkdB →	branched‑chain alpha‑keto acid dehydrogenase complex lipoamide acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,995,523	0	G	C	7.6%	186.7 / 4.7	62	R396P (CGC→CCC) ‡	bkdB	branched‑chain alpha‑keto acid dehydrogenase complex lipoamide acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (27/30);  new base C (3/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGGGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATG  >  NC_002947/4995377‑4995668                                                                                                                                                   |                                                                                                                                                   ccTGGGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACcgcg                                                                                                                                                  <  6:236968/149‑1 (MQ=255) ccTGGGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCATCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACcgcg                                                                                                                                                  >  4:185746/1‑149 (MQ=255)   tGGGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATAGTCGGGGTCAAACGCATGGTAGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTg                                                                                                                                                >  7:57189/1‑149 (MQ=255)         gCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACGCGGTGGTCGaa                                                                                                                                          <  3:114852/149‑2 (MQ=255)         gCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGa                                                                                                                                           <  1:68484/148‑1 (MQ=255)         gCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGAt                                                                                                                                          <  3:325657/149‑1 (MQ=255)          cATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATg                                                                                                                                         <  8:353962/149‑1 (MQ=255)          cATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATg                                                                                                                                         <  4:311997/149‑1 (MQ=255)           aTTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATgg                                                                                                                                        >  1:195048/1‑149 (MQ=255)               tCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATg                                                                                                                                    >  1:338634/1‑149 (MQ=255)                cAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATg                                                                                                                                    >  5:125830/1‑148 (MQ=255)                 aGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATgg                                                                                                                                   <  4:173339/148‑1 (MQ=255)                        cGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑gccgcc                                                                                                                          >  5:318529/1‑149 (MQ=255)                        cGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑gccgcc                                                                                                                          <  6:125830/149‑1 (MQ=255)                        cGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑gccgcc                                                                                                                          <  4:212175/149‑1 (MQ=255)                          gtggcCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCt                                                                                                                        <  7:16096/149‑1 (MQ=255)                                 acacCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATc                                                                                                                 <  5:122843/149‑1 (MQ=255)                                     cccGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAgg                                                                                                             >  5:30887/1‑149 (MQ=255)                                      ccGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGc                                                                                                            >  1:191589/1‑149 (MQ=255)                                        ggAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCg                                                                                                          <  6:30011/149‑1 (MQ=255)                                                         gggTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGaa                                                                                         >  5:64898/1‑149 (MQ=255)                                                              aaCCGCATGGCCGAACGGCCAGTGGTGATCGACAGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGaacaac                                                                                     <  5:252328/148‑1 (MQ=255)                                                                ccGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCg                                                                                  >  7:72201/1‑149 (MQ=255)                                                                  gCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgcc                                                                                >  2:200879/1‑149 (MQ=255)                                                                   cATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgcct                                                                               >  5:365393/1‑149 (MQ=255)                                                                      ggTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑gc                                                                                                                              >  5:347758/1‑99 (MQ=255)                                                                      ggTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑gc                                                                                                                              <  6:347758/99‑1 (MQ=255)                                                                        tCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGcctgcctg                                                                          >  8:272871/1‑149 (MQ=255)                                                                                ccAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTATTCGTGGa                                                                  <  6:30887/149‑1 (MQ=255)                                                                                      gtgATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATc                                                                                                                 <  1:57057/96‑1 (MQ=255)                                                                                      gtgATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATc                                                                                                                 >  2:57057/1‑96 (MQ=255)                                                                                      gtgATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAg                                                             >  8:187317/1‑148 (MQ=255)                                                                                      gtgATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGc                                                            <  1:200879/149‑1 (MQ=255)                                                                                       tgATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCa                                                           >  8:290032/1‑149 (MQ=255)                                                                                         aTCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCGAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCAt                                                          <  6:318529/148‑1 (MQ=255)                                                                                                gCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACa                                                    <  4:163508/147‑1 (MQ=255)                                                                                                gCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGa                                                  <  8:72201/149‑1 (MQ=255)                                                                                                 ccAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgcctgc                                                                             <  7:19129/121‑1 (MQ=255)                                                                                                 ccAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCgcctgc                                                                             >  8:19129/1‑121 (MQ=255)                                                                                                 ccAGATCGTCGTGCGCAAGATGATGAACCAGTCCAGCTCGTTCGACCACCCCGTGGTAGATGGCATGGAT‑TCCGCCCTGTTCATACATGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGAt                                                 >  1:218588/1‑149 (MQ=255)                                                                                                     aTCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATc                                             >  5:114850/1‑149 (MQ=255)                                                                                                      tcgtcgTGCGCAAGATGAAGAACCTGTCCAGCTCGTTCGACAAACGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGAAGTACATGGCCTGCTCGAAAAACACGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTAta                                             >  6:23759/1‑147 (MQ=255)                                                                                                        gtcgtGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCa                                           <  2:195048/148‑1 (MQ=255)                                                                                                         tcgtGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGa                                         <  8:39678/149‑1 (MQ=255)                                                                                                          cgtGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGGGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGAc                                        <  6:64898/149‑1 (MQ=255)                                                                                                             gcgcAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTAc                                     >  3:34712/1‑149 (MQ=255)                                                                                                                 aaGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGATGGCCGCCCTGTTCATCCAGGCCGTACCTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCt                                  >  1:289640/1‑149 (MQ=255)                                                                                                                   gatgatGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGtt                               >  6:10932/1‑149 (MQ=255)                                                                                                                   gatgatGAACCTGTCCAGCTCGTGCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGtt                               <  2:327280/149‑1 (MQ=255)                                                                                                                    atgatgAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGc                                                                                              <  3:310235/85‑1 (MQ=255)                                                                                                                    atgatgAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGc                                                                                              >  4:310235/1‑85 (MQ=255)                                                                                                                       atgaACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGatc                           <  6:365393/149‑1 (MQ=255)                                                                                                                       atgaACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGatc                           <  6:220771/149‑1 (MQ=255)                                                                                                                                tCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATcggcggc                  >  3:53691/1‑149 (MQ=255)                                                                                                                                  cAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATcggcggcgg                >  2:197747/1‑149 (MQ=255)                                                                                                                                  cAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATcggcggcg                 <  2:289640/148‑1 (MQ=255)                                                                                                                                  cAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATcggcggcg                 <  3:185746/148‑1 (MQ=255)                                                                                                                                        catCGACCACGCGGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTgg          >  5:279686/3‑149 (MQ=255)                                                                                                                                          tCGACCACGCGGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGa                             >  8:143031/1‑128 (MQ=255)                                                                                                                                          tCGACCACGCGGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGa                             <  7:143031/128‑1 (MQ=255)                                                                                                                                             accaccGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCt     <  6:114850/149‑1 (MQ=255)                                                                                                                                               caccGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTAt   >  7:271700/1‑149 (MQ=255)                                                                                                                                                accGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATg  <  1:197747/149‑1 (MQ=255)                                                                                                                                                 ccGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATg  >  8:325712/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCTGGGTGGCATTGTCAGCACGCCGGTGGTCAACACCCCGGAAGTGGCAATCGTCGGGGTCAACCGCATGGTCGAACGGCCAGTGGTGATCGACGGCCAGATCGTCGTGCGCAAGATGATGAACCTGTCCAGCTCGTTCGACCACCGCGTGGTCGATGGCATGGAT‑GCCGCCCTGTTCATCCAGGCCGTACGTGGCCTGCTCGAACAACCCGCCTGCCTGTTCGTGGAGTGAGCATGCAACAGATTATCCAGACTACCCTGTTGATCATCGGCGGCGGCCCTGGCGGCTATG  >  NC_002947/4995377‑4995668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑