Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,055,530 C→A 8.4% G266V (GGG→GTG)  gsiC ← glutathione ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,055,5300CA 8.4% 155.0 / 4.0 56G266V (GGG→GTG) gsiCglutathione ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base A (3/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

TCATTTGATTTTCACCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGACGAACACCT  >  NC_002947/5055385‑5055673
                                                                                                                                                 |                                                                                                                                               
tCATTTGATTTTCACCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTccc                                                                                                                                                >  6:255574/1‑147 (MQ=255)
   tttGATTTTCACCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCACCAGAc                                                                                                                                           >  6:78897/1‑149 (MQ=255)
             aCCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGt                                                                                                                                  <  6:131433/148‑1 (MQ=255)
             aCCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGt                                                                                                                                  >  2:229081/1‑148 (MQ=255)
             aCCCGAGGATCAACCAAACCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGt                                                                                                                                  <  7:263574/148‑1 (MQ=255)
               ccGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAggg                                                                                                                                      >  2:57868/1‑142 (MQ=255)
               ccGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAggg                                                                                                                                      <  1:57868/142‑1 (MQ=255)
                cGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTaaa                                                                                                                              <  6:95450/149‑1 (MQ=255)
                    gATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCACCAGACCAGGGCGGTTAAacac                                                                                                                           >  7:261226/1‑148 (MQ=255)
                         accaaccCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTGCCCAGACCAGGGCGGTTAAACACGATTTc                                                                                                                     >  5:333026/1‑149 (MQ=255)
                          ccaaccCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCg                                                                                                                    <  8:91798/149‑1 (MQ=255)
                           caaccCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCgg                                                                                                                   >  3:311661/1‑149 (MQ=255)
                            caccCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGt                                                                                                                  <  4:162937/148‑1 (MQ=255)
                            aaccCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGt                                                                                                                  <  7:86332/149‑1 (MQ=255)
                             accCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGtt                                                                                                                 <  5:170025/149‑1 (MQ=255)
                                 aTAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGa                                                                                                             >  3:104838/1‑149 (MQ=255)
                                           tCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGc                                                                                                    <  4:311661/148‑1 (MQ=255)
                                           tCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCACCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGc                                                                                                    <  8:261226/148‑1 (MQ=255)
                                             ggTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGa                                                                                                 >  7:182238/1‑149 (MQ=255)
                                                gatgatGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGAt                                                                                                >  2:135758/1‑147 (MQ=255)
                                                 atgatgTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGa                                                                                              <  5:255574/148‑1 (MQ=255)
                                                   gatgTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGtt                                                                                                      >  3:178575/1‑138 (MQ=255)
                                                   gatgTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGtt                                                                                                      <  4:178575/138‑1 (MQ=255)
                                                       tttACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGGCGATGAGCGTcc                                                                                        >  8:92403/1‑148 (MQ=255)
                                                       tttACGACGATCACAAACAGTGCGAAGACGACCATCAGCCCTTGGAGAAGGGTGTAATCTCGGGTGTTGAGAGAACCCAAAATCAATTACCCCAGACCAGGGAGGTTAAACACGAATTCGGTTAGGACGGAGTTGCCGAGTAGCGTcc                                                                                        >  6:190329/1‑148 (MQ=255)
                                                               gaTCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACa                                                                               <  1:229081/149‑1 (MQ=255)
                                                               gaTCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACa                                                                               <  7:87645/149‑1 (MQ=255)
                                                                 tCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAgg                                                                             >  3:286004/1‑149 (MQ=255)
                                                                     acacaGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCcta                                                                         >  1:138000/1‑149 (MQ=255)
                                                                     acacaGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCcta                                                                         >  8:77988/1‑149 (MQ=255)
                                                                       acaGTGCGAAGACGACCATCAGCCCTTGGATCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCctact                                                                       <  6:201056/149‑1 (MQ=255)
                                                                       acaGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTT‑AACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCctacta                                                                      >  7:3444/1‑149 (MQ=255)
                                                                        caGTGCGAAGACGACCATCAGCCCTTGGAGTAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCctacta                                                                      >  8:187192/1‑149 (MQ=255)
                                                                               aaGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCAACAAAAATCAAATTCCACAGACCAGGGAGGTTAAACACGAATTCGGTTAGGACGGATTTGCAGATGAGCGTCACAAAATACAGGCATACTACGGttc                                                                >  5:341754/1‑146 (MQ=255)
                                                                                       cATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACACAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCAGTTAGGACGGAGTTGCAGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGa                                                       >  6:6002/1‑149 (MQ=255)
                                                                                             cccTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCATGGCTGTTAAACACGATTTCGGTTAGGACGTAGTTGCCGATGAGCGTCCCAAAATACAGGACTACTACGGTGACAATCGGGATCAGTg                                                 <  5:190329/149‑1 (MQ=255)
                                                                                              ccTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACACAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTg                                                 >  7:265738/1‑148 (MQ=255)
                                                                                                  ggAGCAGGGTGTAATCTCGGGTTTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCAtt                                             <  6:333026/148‑1 (MQ=255)
                                                                                                  ggAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCACCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTa                                            >  3:125911/1‑149 (MQ=255)
                                                                                                       aGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTT‑AACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGc                                      <  8:3444/149‑1 (MQ=255)
                                                                                                        gggTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGc                                      >  1:178961/1‑149 (MQ=255)
                                                                                                               aTCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGcc                               >  3:338154/1‑149 (MQ=255)
                                                                                                               aTCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGc                                <  2:178961/148‑1 (MQ=255)
                                                                                                               aTCTCGGGTGTTGAGCGCACACAAAATCAATTTCCCCAGACCAGGTCGTTTAAACACGATTTATGTTAGGACGGAGTTGCAGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGTATAAGTGCATTACGCAGCCCATGc                                <  6:341754/148‑1 (MQ=255)
                                                                                                                  tcGGGTGTTGCGCGCACCCAAAATCAATTTCCCCCGACCAGGGCGGTTAAACACGAGTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCCAACTACAGGCCTACGACGGTGACAATCGGGCTCAGTGCATTCCGCAGCCCAGGCCTc                             >  2:219463/1‑148 (MQ=255)
                                                                                                                    gggTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTc                             <  7:77988/146‑1 (MQ=255)
                                                                                                                    gggTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAac                          <  7:187192/149‑1 (MQ=255)
                                                                                                                          tGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAAt                    >  1:369232/1‑149 (MQ=255)
                                                                                                                            aGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGACCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCg                  >  1:246342/1‑149 (MQ=255)
                                                                                                                             gcgcACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCa                                                    <  1:109436/114‑1 (MQ=255)
                                                                                                                             gcgcACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCa                                                    >  2:109436/1‑114 (MQ=255)
                                                                                                                             gcgcACCCAAAATCAATTTCACCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGc                 <  4:125911/149‑1 (MQ=255)
                                                                                                                                 aCCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAg             <  4:104838/149‑1 (MQ=255)
                                                                                                                                    cAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGAc          >  3:87237/1‑149 (MQ=255)
                                                                                                                                     aaaaTCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGAc          >  2:251491/1‑148 (MQ=255)
                                                                                                                                        aTCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGACGAac      <  4:338154/149‑1 (MQ=255)
                                                                                                                                            aTTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGACGAACACct  >  4:142048/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCATTTGATTTTCACCCGAGGATCAACCAACCCATAAACGATGTCGGTGATGATGTTTACGACGATCACACACAGTGCGAAGACGACCATCAGCCCTTGGAGCAGGGTGTAATCTCGGGTGTTGAGCGCACCCAAAATCAATTTCCCCAGACCAGGGCGGTTAAACACGATTTCGGTTAGGACGGAGTTGCCGATGAGCGTCCCAAAATACAGGCCTACTACGGTGACAATCGGGATCAGTGCATTACGCAGCCCATGCCTCAACACCAATCGCATAGGACGAACACCT  >  NC_002947/5055385‑5055673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑