Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,092,539 G→A 9.1% intergenic (‑235/+102) astC ← / ← PP_4482 acetylornithine aminotransferase/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,092,5390GA 9.1% 120.6 / 2.8 44intergenic (‑235/+102)astC/PP_4482acetylornithine aminotransferase/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base A (2/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

GACAACTTGTTACAGCGCAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCT  >  NC_002947/5092395‑5092682
                                                                                                                                                |                                                                                                                                               
gACAACTTGTTTCAGCGCAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGt                                                                                                                                             >  8:263867/1‑149 (MQ=255)
  cAACTAGTTACAGCGCAAAACCCAGTTTTCACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGTGAGAGTGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTg                                                                                                                                           <  5:73741/149‑1 (MQ=255)
      ttGTTACAGCGCAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGAtgt                                                                                                                                       >  1:296521/1‑149 (MQ=255)
             aGCGCAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACg                                                                                                                                <  4:711/149‑1 (MQ=255)
               cgcAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACggg                                                                                                                              <  6:56453/149‑1 (MQ=255)
                  aaaaCCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACggg                                                                                                                              >  3:250311/1‑146 (MQ=255)
                     aCCCAGGTTTGACAAGGCTGTCGCCAATGCGACAAACACTGTCGCACTCGCGCAGCGTACGGGCGAGCGCGGGCTGGGCGAACGGGTGCTCACCCAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATc                                                                                                                        <  1:272362/149‑1 (MQ=255)
                       ccAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATc                                                                                                                        >  1:65268/1‑147 (MQ=255)
                         aGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAgc                                                                                                                    <  6:175757/149‑1 (MQ=255)
                           ttttGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGTAGATCGAgcgc                                                                                                                  >  7:146773/1‑149 (MQ=255)
                                  aaGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGCCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTgg                                                                                                           >  3:265647/1‑149 (MQ=255)
                                      cTTTCGGCAATGCGACCAACACGGTCGCAATCGCGCTGTGTACGGGTGAGCGGTGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGggcgg                                                                                                       <  4:265647/149‑1 (MQ=255)
                                               aTGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGAtt                                                                                              <  4:250311/149‑1 (MQ=255)
                                                          acTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAac                                                                                   <  2:65268/149‑1 (MQ=255)
                                                           cTGTCGCATTCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTgc                                                                                           <  6:218521/140‑1 (MQ=255)
                                                           cTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTgcgagattcg                                                                                   >  5:218521/1‑141 (MQ=255)
                                                            tGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAAGGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAaccc                                                                                 >  5:271954/1‑147 (MQ=255)
                                                              tCGCAATCGCGCCGGGGACGGGCGCGCGGGGGCTGGGTGAACGGGGGCTCACACAGGCTGGCGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACAcc                                                                                <  5:106046/148‑1 (MQ=255)
                                                              ccGCTATCGCGCTGTGTACGGGAGACAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGCGGGGGCTGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAacac                                                                                 <  8:207353/146‑1 (MQ=255)
                                                                gCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAAc                                                                             <  8:6219/149‑1 (MQ=255)
                                                                gCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCAATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTg                                                                                                            <  6:269402/118‑1 (MQ=255)
                                                                gCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCAATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTg                                                                                                            <  6:274589/118‑1 (MQ=255)
                                                                gCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCAATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTg                                                                                                            >  5:274589/1‑118 (MQ=255)
                                                                gCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCAATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTg                                                                                                            >  5:269402/1‑118 (MQ=255)
                                                                      gcgcAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAg                                                                       >  1:19018/1‑149 (MQ=255)
                                                                         cAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCa                                                                    <  2:296521/149‑1 (MQ=255)
                                                                            tgtACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATa                                                                  >  3:73301/1‑148 (MQ=255)
                                                                                    agagagGGGGCTGGCGGACCGGGCGCGCACACAGCATTCGGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCCCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGaa                                                         <  8:146773/149‑1 (MQ=255)
                                                                                             gCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATa                                                                  <  1:240895/131‑1 (MQ=255)
                                                                                             gCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATa                                                                  >  2:240895/1‑131 (MQ=255)
                                                                                             gCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGa                                                <  4:318629/149‑1 (MQ=255)
                                                                                                       cGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGcc                                      >  8:197806/1‑149 (MQ=255)
                                                                                                           tGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCttt                                  <  6:27520/149‑1 (MQ=255)
                                                                                                                acacaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACACCGAACCCACCCGAAATCTCAGCCCTTTTCAg                              >  7:255510/1‑148 (MQ=255)
                                                                                                                acaacggATTGTGAGTGGGCAGGATGTACGTCGATGTTGATGTGGTGACGGTGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCtttt                                 <  8:255510/140‑1 (MQ=255)
                                                                                                                  acaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATacac                                                               >  6:274966/1‑113 (MQ=255)
                                                                                                                  acaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATacac                                                               <  5:274966/113‑1 (MQ=255)
                                                                                                                  acaGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCg                           >  5:47017/1‑149 (MQ=255)
                                                                                                                             aGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCt                 <  7:197806/148‑1 (MQ=255)
                                                                                                                                 ggCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCTGCGCTCGAATTGCGCCCCAACTCCAACCCCAAGGCATACACCCCGAACCCCCCCGAAATCTCAGCCCTTTTCAGCACGCGCCGAGCTCAAt             >  5:259525/1‑148 (MQ=255)
                                                                                                                                    aGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAAACCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCa          >  7:207878/1‑148 (MQ=255)
                                                                                                                                          tACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACGCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACggg    <  4:73301/148‑1 (MQ=255)
                                                                                                                                          tACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGgc   <  2:19018/149‑1 (MQ=255)
                                                                                                                                           aCGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGgct  >  7:74069/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GACAACTTGTTACAGCGCAAAACCCAGTTTTGACAAGGCTTTCGGCAATGCGACAAACACTGTCGCAATCGCGCAGTGTACGGGAGAGAGGGGGCTGGGTGAACGGGTGCTCACACAGGATTGTGAGGGGGCAGGATGTACGGCGATGTTGATGTGTTGACGGGGAGATCGAGCGCCGCGTGGGCGGCGCTCGATTTGCGCCCCAACACCAACCCCAAGGCATACACCCCGAACCCACCCGAAATCTCAGCCCTTTTCAGCCGGCGCCGAGCTCAATTCAAACGGGCT  >  NC_002947/5092395‑5092682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑