Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,140,859 C→T 5.9% A235T (GCC→ACC)  PP_4525 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,140,8590CT 5.9% 205.1 / 3.3 68A235T (GCC→ACC) PP_4525LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base T (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TGTTGCAGGTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGT  >  NC_002947/5140714‑5141003
                                                                                                                                                 |                                                                                                                                                
tgttgCAGGTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCca                                                                                                                                                <  5:13416/148‑1 (MQ=255)
     cAGGTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCatcatc                                                                                                                                           <  8:208158/148‑1 (MQ=255)
      aGGTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGc                                                                                                                                         >  8:177408/1‑149 (MQ=255)
        gTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGCTCGAACGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCca                                                                                                                                       >  7:248655/1‑149 (MQ=255)
        gTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCca                                                                                                                                       >  2:25588/1‑149 (MQ=255)
        gTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCca                                                                                                                                       >  8:152931/1‑149 (MQ=255)
          cGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCcaca                                                                                                                                     >  1:216137/1‑149 (MQ=255)
             tCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCAcaccc                                                                                                                                  >  3:158864/1‑149 (MQ=255)
                 tGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGTCATCATCGCCAcacccgcac                                                                                                                              >  3:345088/1‑149 (MQ=255)
                 tGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGTCATCATCGCCAcacccgcac                                                                                                                              >  3:339562/1‑149 (MQ=255)
                    cTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCCACTGGGCCATCATCGCCAcacccgcacccg                                                                                                                           <  1:34734/149‑1 (MQ=255)
                     tGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACacccgcacccgc                                                                                                                          >  4:102768/1‑149 (MQ=255)
                       cAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGa                                                                                                                        <  6:291242/149‑1 (MQ=255)
                       cAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGa                                                                                                                        >  1:322477/1‑149 (MQ=255)
                        aGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGAt                                                                                                                       <  8:258359/149‑1 (MQ=255)
                         gATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGAt                                                                                                                       >  6:189899/1‑148 (MQ=255)
                             aGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATcaca                                                                                                                   <  4:50412/148‑1 (MQ=255)
                              gCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATcacaca                                                                                                                 <  3:102768/149‑1 (MQ=255)
                               ccccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAg                                                                                                                <  1:135338/149‑1 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATca                                                                                                                     <  6:242331/142‑1 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATca                                                                                                                     >  5:242331/1‑142 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATc                                                                                                                      <  5:138933/141‑1 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATc                                                                                                                      >  6:138933/1‑141 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAg                                                                                                                <  6:99701/147‑1 (MQ=255)
                                 ccGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAg                                                                                                                >  5:99701/1‑147 (MQ=255)
                                      cATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCa                                                                                                         <  6:28462/149‑1 (MQ=255)
                                           cTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACCCAGGCCAGCATGctc                                                                                                    >  4:327963/1‑149 (MQ=255)
                                           cTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGctc                                                                                                    <  4:158864/149‑1 (MQ=255)
                                           cTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGctc                                                                                                    >  8:12174/1‑149 (MQ=255)
                                           cTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGctc                                                                                                    <  5:135483/149‑1 (MQ=255)
                                                 gcCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTAc                                                                                              <  2:216137/149‑1 (MQ=255)
                                                    acaTCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGAc                                                                                           <  2:322459/149‑1 (MQ=255)
                                                    acaTCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGAc                                                                                           <  5:189899/149‑1 (MQ=255)
                                                     caTCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACt                                                                                          >  2:287503/1‑149 (MQ=255)
                                                      aTCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTc                                                                                         >  4:247418/1‑149 (MQ=255)
                                                          gCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGAtc                                                                                     >  8:213528/1‑149 (MQ=255)
                                                          gCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGAtc                                                                                     >  3:132732/1‑149 (MQ=255)
                                                           ccAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCGATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGAtct                                                                                    >  7:284113/1‑149 (MQ=255)
                                                             aGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTcc                                                                                  <  6:302268/149‑1 (MQ=255)
                                                             aGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTcc                                                                                  <  2:322477/149‑1 (MQ=255)
                                                                   ccGCCTGATCGAAAGGTGCCTGCCGCGAGTGCACCCTCCCCCGATCACGCCCCGGCAGGCTGTCGAGCCGCGCCTGGGCCCTCAGCGCCCCCCCCGCACCCGCGATCACCCAGGCCAGCATGCTCGGGGACGCCGCGAGCGCCATcccc                                                                            >  3:20700/1‑149 (MQ=255)
                                                                       cTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGcc                                                                        >  5:230172/1‑149 (MQ=255)
                                                                         gATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGccc                                                                       <  1:213883/148‑1 (MQ=255)
                                                                         gATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGccc                                                                       >  2:213883/1‑148 (MQ=255)
                                                                             gAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTg                                                                  <  5:377385/149‑1 (MQ=255)
                                                                             gAAAGGAGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTg                                                                  <  8:60742/149‑1 (MQ=255)
                                                                                  gTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCCCCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTa                                                             >  3:319573/1‑149 (MQ=255)
                                                                                  gTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTa                                                             <  7:152931/149‑1 (MQ=255)
                                                                                     ccTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTatgg                                                          <  7:213528/149‑1 (MQ=255)
                                                                                        gCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTa                                                             <  1:195298/143‑1 (MQ=255)
                                                                                        gCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTa                                                             >  2:195298/1‑143 (MQ=255)
                                                                                        gCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGATGTCTAGCATCGACTGCGCCATCATCGCCACACCCGCACCCGCGATCACACAGGACAGCATGCTCTGGAACGACTAGATCAAAATCACCCGCGCCATTGCCGTatgggca                                                       >  7:289518/1‑149 (MQ=255)
                                                                                                  cACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATAc                                              <  7:12174/148‑1 (MQ=255)
                                                                                                      cTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGc                                         <  3:247418/149‑1 (MQ=255)
                                                                                                      cTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGc                                         <  4:132732/149‑1 (MQ=255)
                                                                                                       tCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGcc                                        >  6:202103/1‑149 (MQ=255)
                                                                                                                   gCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACCCAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATg                            >  3:137077/1‑149 (MQ=255)
                                                                                                                           aggcTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCAAGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGa                    >  2:122409/1‑149 (MQ=255)
                                                                                                                            ggcTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGag                   <  5:143687/149‑1 (MQ=255)
                                                                                                                                 tCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGcc              >  8:178382/1‑149 (MQ=255)
                                                                                                                                   gAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCt            >  2:75201/1‑149 (MQ=255)
                                                                                                                                        tCGACTGGGTCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCgg       <  4:339562/149‑1 (MQ=255)
                                                                                                                                        tCGACTGGGTCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCgg       <  4:345088/149‑1 (MQ=255)
                                                                                                                                           aCTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAg    >  8:118916/1‑149 (MQ=255)
                                                                                                                                           aCTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAg    >  5:227311/1‑149 (MQ=255)
                                                                                                                                           aCTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAg    <  6:227311/149‑1 (MQ=255)
                                                                                                                                               ggCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGt  <  1:75201/147‑1 (MQ=255)
                                                                                                                                               ggCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGt  <  1:287503/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTTGCAGGTCGATCCATGCCTGCAGATTAGCCCCGCGCATACCTTGGCGCCACATCAGCCAGGTGACCGCCTGATCGAAAGGTGCCTGCAGCGAGTGCACCCTCACCCGATCACGCCCAGGCAGGCTGTCGAGCATCGACTGGGCCATCATCGCCACACCCGCACCCGCGATCACACAGGCCAGCATGCTCTGGTACGACTCGATCTCCATCACCCGCCCCATTGGCGTATGGGCATGGGCATACCAGGCCTCCAGACGCATGCGGTACGAGCAGCCCTGGCGGAAGGT  >  NC_002947/5140714‑5141003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑