Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,233,543 T→C 9.9% V136A (GTC→GCC)  PP_4611 → ECF family RNA polymerase sigma‑70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,233,5430TC 9.9% 187.9 / 9.1 72V136A (GTC→GCC) PP_4611ECF family RNA polymerase sigma‑70 factor
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base C (5/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

GCCTACCTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATT  >  NC_002947/5233395‑5233691
                                                                                                                                                    |                                                                                                                                                    
gCCTACCTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGt                                                                                                                                                      >  6:166512/1‑149 (MQ=255)
      cTGCAAGCGCTCTCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACgg                                                                                                                                                <  4:287554/149‑1 (MQ=255)
         cAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGc                                                                                                                                               <  7:83094/147‑1 (MQ=255)
           aGCGCTCGCACAGGTCCCGGAAGAGGGCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTgg                                                                                                                                           <  1:104776/149‑1 (MQ=255)
             cgcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGc                                                                                                                                         <  7:27821/149‑1 (MQ=255)
              gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGc                                                                                                                                         >  4:316189/1‑148 (MQ=255)
                tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGcc                                                                                                                                        >  1:287609/1‑147 (MQ=255)
                 cGCACAGGTCCCGGAAGAGTTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg                                                                                                                                     <  2:102854/149‑1 (MQ=255)
                  gCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg                                                                                                                                    <  1:257867/149‑1 (MQ=255)
                   cacaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCagca                                                                                                                                    <  7:227312/148‑4 (MQ=255)
                   cacaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCag                                                                                                                                      >  8:227312/1‑145 (MQ=255)
                   cacaGGGCCCGGAAGAGGTCGCGCCGGGCTAGGAACAGATGGCGAGCATCTGCGAGACATTGCTCGGGCTCGACCCCTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGc                                                                                                                                   <  7:152135/149‑1 (MQ=255)
                    acaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGGCagcag                                                                                                                                   >  3:120729/1‑144 (MQ=255)
                       ggTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGaaa                                                                                                                               <  5:519/149‑1 (MQ=255)
                        gTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt                                                                                                                              >  7:234137/1‑149 (MQ=255)
                         tCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtc                                                                                                                             <  4:315916/149‑1 (MQ=255)
                           ccGGAAGAGTTCGCGCCGTGCGAGTAACAGACGGCTATCATCTTCTCCACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgc                                                                                                                           <  8:212577/149‑1 (MQ=255)
                           ccGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgc                                                                                                                           >  8:287998/1‑149 (MQ=255)
                               aaGAGGGCGCGCCGTGCGAGTAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCCACCATTTTCTGGCCGGCCTTCCACCGTTGGTCAACCGCGCCTTCCTGCTGCCCCTGGTCGACGGCCTGGGCCAAGGCGAAAtcgctcgc                                                                                                                       <  2:334453/149‑1 (MQ=255)
                                       gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTggg                                                                                                               <  7:287998/149‑1 (MQ=255)
                                       gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTggg                                                                                                               <  1:357230/149‑1 (MQ=255)
                                         gcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCa                                                                                                             >  3:218850/1‑149 (MQ=255)
                                          ccGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAt                                                                                                            <  8:95291/149‑1 (MQ=255)
                                             tGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAAc                                                                                                                   >  3:45666/1‑139 (MQ=255)
                                             tGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAAc                                                                                                                   <  4:45666/139‑1 (MQ=255)
                                                gAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCt                                                                                                      <  7:311640/149‑1 (MQ=255)
                                                 aGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTg                                                                                                     <  8:310120/149‑1 (MQ=255)
                                                  ggAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGCCGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTgg                                                                                                    >  3:99570/1‑149 (MQ=255)
                                                   gAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGc                                                                                                   <  5:166512/149‑1 (MQ=255)
                                                     aCAGAAGGCGATCATCTTCGAGACATAGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGACAAACGCGCCTTCCTGCTGCCCCAGGTCGACCGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATATCGCTGCCCa                                                                                                 >  7:295729/1‑149 (MQ=255)
                                                        gAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCAACgg                                                                                              >  1:94715/1‑149 (MQ=255)
                                                              gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc                                                                                        >  8:351882/1‑149 (MQ=255)
                                                              gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc                                                                                        <  2:210430/149‑1 (MQ=255)
                                                              gATAATATTCGAGACATTGCTAGCGCTCGAACACTTGCTGGACGGCCTTCCACCGTTTGTCAAACGCGCCTTCATGCTGGCCCATGTCGACGGCCTGGGCAAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTTGCCACTGTCAAgc                                                                                        >  5:112050/1‑149 (MQ=255)
                                                                  atcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCTCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTa                                                                                    <  8:267177/149‑1 (MQ=255)
                                                                    cTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGTTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAcc                                                                                  >  7:1496/1‑149 (MQ=255)
                                                                     ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACgg                                                                                              <  1:234699/136‑1 (MQ=255)
                                                                     ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACgg                                                                                              >  2:234699/1‑136 (MQ=255)
                                                                     ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCt                                                                                 >  4:315702/1‑149 (MQ=255)
                                                                      tCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGATACCt                                                                                 >  4:199868/1‑148 (MQ=255)
                                                                       cGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTg                                                                                >  7:23659/1‑148 (MQ=255)
                                                                         agaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGaa                                                                              >  3:363361/1‑148 (MQ=255)
                                                                         agaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTCGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATAGCTCGCGAACTGGGCATTACGCTGGCCACGGTCAAGAGCTACCTgca                                                                              >  6:9995/1‑146 (MQ=255)
                                                                             aTTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAgg                                                                         >  4:365731/1‑149 (MQ=255)
                                                                                      gcTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTCCCTGAACACGGCCGCCAt                                                                  >  8:326847/1‑147 (MQ=255)
                                                                                        tCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATgcgc                                                              >  5:340541/1‑149 (MQ=255)
                                                                                        tCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGGCAAGCGCTACCTGAACAAGGCAGCCATgcgc                                                              >  8:341345/1‑149 (MQ=255)
                                                                                             cACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTa                                                         >  4:215008/1‑149 (MQ=255)
                                                                                                     ggACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCgcgc                                                 >  7:8163/1‑149 (MQ=255)
                                                                                                      gACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCt                                                <  8:271127/149‑1 (MQ=255)
                                                                                                           ccTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa                                           >  8:222108/1‑149 (MQ=255)
                                                                                                           ccTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa                                           <  8:234137/149‑1 (MQ=255)
                                                                                                            cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAAc                                          >  1:151838/1‑149 (MQ=255)
                                                                                                             ttCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACg                                         >  3:234769/1‑149 (MQ=255)
                                                                                                                  ccGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCGAAGGCGAAATCGCTCGCGAACTGGTCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCg                                    <  4:218850/149‑1 (MQ=255)
                                                                                                                        gTCAAACGCGGCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTcc                              <  2:318698/149‑1 (MQ=255)
                                                                                                                        gTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc                                                                     >  5:330212/1‑110 (MQ=255)
                                                                                                                        gTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc                                                                     <  6:330212/110‑1 (MQ=255)
                                                                                                                             aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                              <  2:220850/128‑1 (MQ=255)
                                                                                                                             aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                              >  1:220850/1‑128 (MQ=255)
                                                                                                                             aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCt                         <  2:151838/149‑1 (MQ=255)
                                                                                                                              cgcgCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtg                        <  8:23659/149‑1 (MQ=255)
                                                                                                                              cgcgCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtg                        <  4:363361/149‑1 (MQ=255)
                                                                                                                                cgcCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtggt                      <  8:8163/149‑1 (MQ=255)
                                                                                                                                 gcCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtggtg                     >  5:321613/1‑149 (MQ=255)
                                                                                                                                      ccTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCg                  <  6:340541/147‑1 (MQ=255)
                                                                                                                                          ttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAg            >  3:66617/2‑149 (MQ=255)
                                                                                                                                          ttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAg            >  5:76855/2‑149 (MQ=255)
                                                                                                                                          ctgGCCCAGGGCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAAc              <  2:287609/147‑1 (MQ=255)
                                                                                                                                             gCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAACCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAgg           <  6:304061/147‑1 (MQ=255)
                                                                                                                                              cccAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCg        >  1:221562/1‑149 (MQ=255)
                                                                                                                                               ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGt       >  2:161415/1‑149 (MQ=255)
                                                                                                                                                 aGGTCGACGGCCTGGGCCTAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCGACGGTCAAGCGCTACCTGAACAAGGCACCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGc     <  8:295729/149‑1 (MQ=255)
                                                                                                                                                    tCGACGGCCTGGGCCAAGGCGAAATCGTTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt  <  8:1496/149‑1 (MQ=255)
                                                                                                                                                    tCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt  <  3:316189/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCTACCTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATT  >  NC_002947/5233395‑5233691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑