Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,233,546 A→T 9.1% D137V (GAC→GTC)  PP_4611 → ECF family RNA polymerase sigma‑70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,233,5460AT 9.1% 204.3 / 9.0 77D137V (GAC→GTC) PP_4611ECF family RNA polymerase sigma‑70 factor
Reads supporting (aligned to +/- strand):  ref base A (32/37);  new base T (5/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

CTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGT  >  NC_002947/5233401‑5233694
                                                                                                                                                 |                                                                                                                                                    
cTGCAAGCGCTCTCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACgg                                                                                                                                                   <  4:287554/149‑1 (MQ=255)
   cAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGc                                                                                                                                                  <  7:83094/147‑1 (MQ=255)
     aGCGCTCGCACAGGTCCCGGAAGAGGGCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTgg                                                                                                                                              <  1:104776/149‑1 (MQ=255)
       cgcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGc                                                                                                                                            <  7:27821/149‑1 (MQ=255)
        gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGc                                                                                                                                            >  4:316189/1‑148 (MQ=255)
          tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGcc                                                                                                                                           >  1:287609/1‑147 (MQ=255)
           cGCACAGGTCCCGGAAGAGTTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg                                                                                                                                        <  2:102854/149‑1 (MQ=255)
            gCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg                                                                                                                                       <  1:257867/149‑1 (MQ=255)
             cacaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCagca                                                                                                                                       <  7:227312/148‑4 (MQ=255)
             cacaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCag                                                                                                                                         >  8:227312/1‑145 (MQ=255)
             cacaGGGCCCGGAAGAGGTCGCGCCGGGCTAGGAACAGATGGCGAGCATCTGCGAGACATTGCTCGGGCTCGACCCCTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGc                                                                                                                                      <  7:152135/149‑1 (MQ=255)
              acaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGGCagcag                                                                                                                                      >  3:120729/1‑144 (MQ=255)
                 ggTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGaaa                                                                                                                                  <  5:519/149‑1 (MQ=255)
                  gTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt                                                                                                                                 >  7:234137/1‑149 (MQ=255)
                   tCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtc                                                                                                                                <  4:315916/149‑1 (MQ=255)
                     ccGGAAGAGTTCGCGCCGTGCGAGTAACAGACGGCTATCATCTTCTCCACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgc                                                                                                                              <  8:212577/149‑1 (MQ=255)
                     ccGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgc                                                                                                                              >  8:287998/1‑149 (MQ=255)
                         aaGAGGGCGCGCCGTGCGAGTAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCCACCATTTTCTGGCCGGCCTTCCACCGTTGGTCAACCGCGCCTTCCTGCTGCCCCTGGTCGACGGCCTGGGCCAAGGCGAAAtcgctcgc                                                                                                                          <  2:334453/149‑1 (MQ=255)
                                 gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTggg                                                                                                                  <  1:357230/149‑1 (MQ=255)
                                 gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTggg                                                                                                                  <  7:287998/149‑1 (MQ=255)
                                   gcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCa                                                                                                                >  3:218850/1‑149 (MQ=255)
                                    ccGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCAt                                                                                                               <  8:95291/149‑1 (MQ=255)
                                       tGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAAc                                                                                                                      <  4:45666/139‑1 (MQ=255)
                                       tGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAAc                                                                                                                      >  3:45666/1‑139 (MQ=255)
                                          gAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCt                                                                                                         <  7:311640/149‑1 (MQ=255)
                                           aGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTg                                                                                                        <  8:310120/149‑1 (MQ=255)
                                            ggAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGCCGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTgg                                                                                                       >  3:99570/1‑149 (MQ=255)
                                             gAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGc                                                                                                      <  5:166512/149‑1 (MQ=255)
                                               aCAGAAGGCGATCATCTTCGAGACATAGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGACAAACGCGCCTTCCTGCTGCCCCAGGTCGACCGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATATCGCTGCCCa                                                                                                    >  7:295729/1‑149 (MQ=255)
                                                  gAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCAACgg                                                                                                 >  1:94715/1‑149 (MQ=255)
                                                        gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc                                                                                           >  8:351882/1‑149 (MQ=255)
                                                        gATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAgc                                                                                           <  2:210430/149‑1 (MQ=255)
                                                        gATAATATTCGAGACATTGCTAGCGCTCGAACACTTGCTGGACGGCCTTCCACCGTTTGTCAAACGCGCCTTCATGCTGGCCCATGTCGACGGCCTGGGCAAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTTGCCACTGTCAAgc                                                                                           >  5:112050/1‑149 (MQ=255)
                                                            atcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCTCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTa                                                                                       <  8:267177/149‑1 (MQ=255)
                                                              cTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGTTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAcc                                                                                     >  7:1496/1‑149 (MQ=255)
                                                               ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACgg                                                                                                 <  1:234699/136‑1 (MQ=255)
                                                               ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACgg                                                                                                 >  2:234699/1‑136 (MQ=255)
                                                               ttCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCt                                                                                    >  4:315702/1‑149 (MQ=255)
                                                                tCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGATACCt                                                                                    >  4:199868/1‑148 (MQ=255)
                                                                 cGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTg                                                                                   >  7:23659/1‑148 (MQ=255)
                                                                   agaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGaa                                                                                 >  3:363361/1‑148 (MQ=255)
                                                                   agaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTCGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATAGCTCGCGAACTGGGCATTACGCTGGCCACGGTCAAGAGCTACCTgca                                                                                 >  6:9995/1‑146 (MQ=255)
                                                                       aTTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAgg                                                                            >  4:365731/1‑149 (MQ=255)
                                                                                gcTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTCCCTGAACACGGCCGCCAt                                                                     >  8:326847/1‑147 (MQ=255)
                                                                                  tCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATgcgc                                                                 >  5:340541/1‑149 (MQ=255)
                                                                                  tCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGGCAAGCGCTACCTGAACAAGGCAGCCATgcgc                                                                 >  8:341345/1‑149 (MQ=255)
                                                                                       cACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTa                                                            >  4:215008/1‑149 (MQ=255)
                                                                                               ggACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCgcgc                                                    >  7:8163/1‑149 (MQ=255)
                                                                                                gACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCt                                                   <  8:271127/149‑1 (MQ=255)
                                                                                                     ccTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa                                              >  8:222108/1‑149 (MQ=255)
                                                                                                     ccTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa                                              <  8:234137/149‑1 (MQ=255)
                                                                                                      cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAAc                                             >  1:151838/1‑149 (MQ=255)
                                                                                                       ttCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACg                                            >  3:234769/1‑149 (MQ=255)
                                                                                                            ccGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCGAAGGCGAAATCGCTCGCGAACTGGTCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCg                                       <  4:218850/149‑1 (MQ=255)
                                                                                                                  gTCAAACGCGGCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTcc                                 <  2:318698/149‑1 (MQ=255)
                                                                                                                  gTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc                                                                        <  6:330212/110‑1 (MQ=255)
                                                                                                                  gTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc                                                                        >  5:330212/1‑110 (MQ=255)
                                                                                                                       aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                                 >  1:220850/1‑128 (MQ=255)
                                                                                                                       aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                                 <  2:220850/128‑1 (MQ=255)
                                                                                                                       aCGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCt                            <  2:151838/149‑1 (MQ=255)
                                                                                                                        cgcgCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtg                           <  4:363361/149‑1 (MQ=255)
                                                                                                                        cgcgCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtg                           <  8:23659/149‑1 (MQ=255)
                                                                                                                          cgcCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtggt                         <  8:8163/149‑1 (MQ=255)
                                                                                                                           gcCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCtggtg                        >  5:321613/1‑149 (MQ=255)
                                                                                                                                ccTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCg                     <  6:340541/147‑1 (MQ=255)
                                                                                                                                    ttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAg               >  3:66617/2‑149 (MQ=255)
                                                                                                                                    ttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAg               >  5:76855/2‑149 (MQ=255)
                                                                                                                                    ctgGCCCAGGGCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAAc                 <  2:287609/147‑1 (MQ=255)
                                                                                                                                       gCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAACCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAgg              <  6:304061/147‑1 (MQ=255)
                                                                                                                                        cccAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCg           >  1:221562/1‑149 (MQ=255)
                                                                                                                                         ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGt          >  2:161415/1‑149 (MQ=255)
                                                                                                                                           aGGTCGACGGCCTGGGCCTAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCGACGGTCAAGCGCTACCTGAACAAGGCACCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGc        <  8:295729/149‑1 (MQ=255)
                                                                                                                                              tCGACGGCCTGGGCCAAGGCGAAATCGTTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt     <  8:1496/149‑1 (MQ=255)
                                                                                                                                              tCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt     <  3:316189/149‑1 (MQ=255)
                                                                                                                                               cGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTg    >  8:69229/1‑149 (MQ=255)
                                                                                                                                                gACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt     >  5:135587/1‑147 (MQ=255)
                                                                                                                                                gACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCAtt     <  6:135587/147‑1 (MQ=255)
                                                                                                                                                 aCGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGt  <  7:222108/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGT  >  NC_002947/5233401‑5233694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑