Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,263,966 A→G 9.5% V94A (GTG→GCG)  zinU ← P‑loop guanosine triphosphatase‑dependent zinc chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,263,9660AG 9.5% 170.1 / 4.9 62V94A (GTG→GCG) zinUP‑loop guanosine triphosphatase‑dependent zinc chaperone
Reads supporting (aligned to +/- strand):  ref base A (24/32);  new base G (4/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTC  >  NC_002947/5263825‑5264114
                                                                                                                                             |                                                                                                                                                    
gTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGc                                                                                                                                                >  3:339490/1‑148 (MQ=255)
       tCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaat                                                                                                                                        <  6:171775/149‑2 (MQ=255)
       tCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAg                                                                                                                                        <  5:347505/149‑1 (MQ=255)
        cAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCTGGTGATCGaatg                                                                                                                                       <  1:163019/149‑3 (MQ=255)
           gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                    <  8:1666/149‑1 (MQ=255)
           gTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGa                                                                                                                                    >  2:25717/1‑149 (MQ=255)
           gTGCAGTTCCGCATGAGCGTCATCAACAAGGCTGATGATGACGACGACGAACTACAGCTCACGCAACTCTTCTTCCATGAAGAAACTTTGCGCGACCGGCGCCGGGTCGGAAAGCCCGCTACATACGATCACCAGGCGATCGAAGGcta                                                                                                                                    >  8:246260/1‑147 (MQ=255)
             gCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGAtt                                                                                                                                  >  4:118890/1‑149 (MQ=255)
               aGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTc                                                                                                                                <  1:242636/149‑1 (MQ=255)
                        tGCGCGGCCTCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATc                                                                                                                       >  5:239901/1‑149 (MQ=255)
                               cATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGt                                                                                                                <  1:25717/149‑1 (MQ=255)
                                   gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTc                                                                                                              >  5:185291/1‑147 (MQ=255)
                                   gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCa                                                                                                             <  4:339490/148‑1 (MQ=255)
                                   gACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATAACCAGGCGATCGAAGGCGATTTCCCCGGCATCCATGCGTTc                                                                                                              >  2:89272/1‑147 (MQ=255)
                                    aCAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAag                                                                                                           >  6:374644/1‑149 (MQ=255)
                                           tgatgaTGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAgg                                                                                                    <  6:185291/149‑1 (MQ=255)
                                           tgatgaTGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAgg                                                                                                    <  7:246260/149‑1 (MQ=255)
                                            gatgatGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGt                                                                                                   <  2:227928/149‑1 (MQ=255)
                                              tgatgCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTa                                                                                                  <  3:245370/148‑1 (MQ=255)
                                               gatgCCGTCGAGGATGTGGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTa                                                                                                  >  5:42456/1‑147 (MQ=255)
                                                atgCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGGCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACa                                                                                                <  2:250104/148‑1 (MQ=255)
                                                    cGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCGCCAGGCGCTCGAGAGCGCTTTGCCCGGCAGCCCGGCGATCAAGCAGAAGGTGCAgctcg                                                                                           >  8:151880/1‑149 (MQ=255)
                                                     gTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAgcgcgc                                                                                          <  3:127594/149‑1 (MQ=255)
                                                         aGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGt                                                                                      <  3:274143/149‑1 (MQ=255)
                                                              gTAGCGCTCACGCAGCTCTTCGTCGATGAAGCACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGt                                                                                 >  5:365008/1‑149 (MQ=255)
                                                               tAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTc                                                                                >  1:231843/1‑149 (MQ=255)
                                                                  cgcTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGAt                                                                                                                                             >  5:228110/1‑85 (MQ=255)
                                                                  cgcTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGAt                                                                                                                                             <  6:228110/85‑1 (MQ=255)
                                                                   gcTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCa                                                                            >  4:183775/1‑149 (MQ=255)
                                                                   gcTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCa                                                                            <  5:374644/149‑1 (MQ=255)
                                                                     tCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATg                                                                          >  1:73832/1‑149 (MQ=255)
                                                                       aCGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCAAGTCAGGTCGCCATGGa                                                                        <  1:15594/149‑1 (MQ=255)
                                                                        cGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCATCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAt                                                                       <  1:43741/149‑1 (MQ=255)
                                                                         gCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGt                                                                                                                >  4:113756/1‑107 (MQ=255)
                                                                         gCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGt                                                                                                                <  3:113756/107‑1 (MQ=255)
                                                                         gCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGAtg                                                                      <  7:55962/149‑1 (MQ=255)
                                                                               ctTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTgcag                                                                >  6:338296/1‑149 (MQ=255)
                                                                                   gtcgATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                            >  5:280315/1‑149 (MQ=255)
                                                                                   gtcgATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATa                                                            <  6:42456/149‑1 (MQ=255)
                                                                                      gATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATaca                                                          >  5:71728/1‑148 (MQ=255)
                                                                                           ataaCGTTTGCGCGACAGGAGCCTGGTCGGACAGGCAGTGGAATTCGCTAACCAGTCGATCGAAGGCGATTTCCCAGGCATCAAGGCGTGCAAGCAGCAGGTACAGCGCGCGAGTCAGGGCGCCATGGATGCTGCAGCATACACAAcc                                                     <  7:156066/146‑1 (MQ=255)
                                                                                             aaCGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGtt                                                  <  5:27472/149‑1 (MQ=255)
                                                                                                    gcgcGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCaa                                            <  6:71728/148‑1 (MQ=255)
                                                                                                    gcgcGACCGGCGCCGGGTCGGACAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAg                                           >  2:67178/1‑149 (MQ=255)
                                                                                                     cgcgACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAgg                                          <  6:365008/149‑1 (MQ=255)
                                                                                                        gACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCa                                       >  4:133566/1‑149 (MQ=255)
                                                                                                          ccGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATg                                     <  3:118890/149‑1 (MQ=255)
                                                                                                            ggCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAATGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                   <  8:211242/149‑1 (MQ=255)
                                                                                                            ggCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAc                                   <  5:338296/149‑1 (MQ=255)
                                                                                                             gcgcCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGAcc                                  >  7:39175/1‑149 (MQ=255)
                                                                                                              cgcCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCt                                 <  6:239901/149‑1 (MQ=255)
                                                                                                                     tCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCgg                          <  3:133566/149‑1 (MQ=255)
                                                                                                                         ccAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGt                      >  4:65861/1‑149 (MQ=255)
                                                                                                                            ggCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTcc                   <  2:231843/149‑1 (MQ=255)
                                                                                                                               cGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGAAGGTACAGAGCGCGAGTCAGGTCGACATGGATGCTGCAGCATACACAACGGTTGGCCAAGGTCATGACCTGCAGCGGCTGTTcccac                >  4:224519/1‑147 (MQ=255)
                                                                                                                                ggTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCca               <  6:280315/149‑1 (MQ=255)
                                                                                                                                    cATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCcagca            <  2:73832/148‑1 (MQ=255)
                                                                                                                                     cttCGATCGCCTGGTGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGTGCGAGTCAGGTCGCCATGGATGCTGCATCATACAAAACCGTTGGCCAAGGTCAGGACCTGCACCGGCTGTTCCCCcagccgg          >  8:351631/2‑146 (MQ=255)
                                                                                                                                     cttCGATCGCCTGGTGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTTGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGt          >  7:240535/2‑149 (MQ=255)
                                                                                                                                     cttCGATCGCCTGGTGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGt          >  6:248087/2‑149 (MQ=255)
                                                                                                                                      ttCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGtt         >  2:18343/1‑149 (MQ=255)
                                                                                                                                         gATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTgg       <  1:18343/148‑1 (MQ=255)
                                                                                                                                             aCCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTc  >  3:306861/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GTCTGGGTCAGGTGCAGTTCGGCATGCGCGGCATCGACAAGGGTGATGATGCCGTCGAGGATGTAGCGCTCACGCAGCTCTTCGTCGATGAAGAACGTTTGCGCGACCGGCGCCGGGTCGGCCAGGCCGGTGCATTCGATCACCAGGCGATCGAAGGCGATTTCCCCGGCATCCAGGCGTTCAAGCAGCAGGTACAGCGCGCGAGTCAGGTCGCCATGGATGCTGCAGCATACACAACCGTTGGCCAAGGTCATGACCTGCACCGGCTGTTCCCCCAGCAGTTGGCTGTC  >  NC_002947/5263825‑5264114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑