Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,269,315 G→C 5.9% L342V (CTC→GTC) ‡ radA ← DNA repair enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,269,3150GC 5.9% 214.3 / 3.5 68L342V (CTC→GTC) ‡radADNA repair enzyme
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base C (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

AAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCGG  >  NC_002947/5269167‑5269462
                                                                                                                                                       |                                                                                                                                                   
aaaCCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAg                                                                                                                                                     <  5:311459/149‑1 (MQ=255)
    cATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTGCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTAGGGCTAACCCCCAT‑GCCGTGGGGCAcc                                                                                                                                                 >  5:123417/1‑149 (MQ=255)
     aTGGGCCAGGGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCg                                                                                                                                                <  7:57239/149‑1 (MQ=255)
      tGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGc                                                                                                                                               <  2:212989/149‑1 (MQ=255)
      tGGGCCAGTGGCATGTTGCGCAATCTCGACATCACCGCCGCCAGCATGGCCATGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTCAGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCg                                                                                                                                                <  1:96903/149‑1 (MQ=255)
         gCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGCTCCACCGCcat                                                                                                                                            <  5:313467/149‑2 (MQ=255)
           cAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGCTCCACCGCcatgg                                                                                                                                          <  3:292478/149‑4 (MQ=255)
            aGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCcaacaa                                                                                                                                         <  5:163957/149‑1 (MQ=255)
             gTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCaacaac                                                                                                                                        <  8:153269/149‑1 (MQ=255)
                gCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATg                                                                                                                                     >  2:16862/1‑149 (MQ=255)
                gCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATg                                                                                                                                     <  5:271650/149‑1 (MQ=255)
                  cGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGc                                                                                                                                   <  8:348903/149‑1 (MQ=255)
                     ttGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGATCC‑GAAGCCGTTTCCAGCACCTTCACACCGCCGACCACGA‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGc                                                                                                                                   >  1:310626/1‑146 (MQ=255)
                           aaGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGtt                                                                                                                          >  7:123065/1‑149 (MQ=255)
                            aGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTc                                                                                                                         <  8:113940/149‑1 (MQ=255)
                            aGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTc                                                                                                                         <  8:300478/149‑1 (MQ=255)
                              cTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTg                                                                                                                       <  1:21862/149‑1 (MQ=255)
                                      cACCGCCGCCAGCAGGGCCAGGTCCGGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAgg                                                                                                                >  1:5544/1‑149 (MQ=255)
                                        ccgccgCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAACCACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGcc                                                                                                              >  7:131836/1‑148 (MQ=255)
                                        ccgccgCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATg                                                                                                                                     <  5:171896/125‑1 (MQ=255)
                                        ccgccgCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGccc                                                                                                             <  2:5544/149‑1 (MQ=255)
                                        ccgccgCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGA‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATg                                                                                                                                     >  6:171896/1‑125 (MQ=255)
                                           ccgccAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCATCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                                                                                          <  8:155235/149‑1 (MQ=255)
                                            cgccAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAgg                                                                                                         <  5:104519/149‑1 (MQ=255)
                                                 gcagGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCTAGACGGTTCTGGTCCAGGCCCAAGGTTAcc                                                                                                    <  1:223213/149‑1 (MQ=255)
                                                   agGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccg                                                                                                  >  8:115893/1‑149 (MQ=255)
                                                     ggCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccgcc                                                                                                >  5:149232/1‑149 (MQ=255)
                                                         aGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCgg                                                                                            <  1:126804/149‑1 (MQ=255)
                                                            tCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGtt                                                                                         <  1:16862/149‑1 (MQ=255)
                                                                gAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TCAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCAAGACGGTTCTGGTCAAGGCCCAAGGTTACCCGCCGCGGGTTgg                                                                                       >  5:374657/1‑148 (MQ=255)
                                                                 aaGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                                      >  3:119310/1‑148 (MQ=255)
                                                                 aaGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGc                                                                                      >  2:135719/1‑148 (MQ=255)
                                                                                 cTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGa                                                                     <  6:149232/149‑1 (MQ=255)
                                                                                  ttCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                                    >  1:126486/1‑149 (MQ=255)
                                                                                    cACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGTAT‑ACCGCCATGGCGGTGGAGCACCGCCAGCAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                                    <  7:124795/147‑1 (MQ=255)
                                                                                    cACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                                    >  6:356475/1‑147 (MQ=255)
                                                                                    cACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                                    <  3:237567/147‑1 (MQ=255)
                                                                                    cACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAc                                                                    <  5:232937/147‑1 (MQ=255)
                                                                                      ccccGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgc                                                                >  3:192263/1‑149 (MQ=255)
                                                                                       cccGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTAccc                                                                                                   <  1:104572/113‑1 (MQ=255)
                                                                                       cccGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTAccc                                                                                                   >  2:104572/1‑113 (MQ=255)
                                                                                       cccGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcg                                                               >  6:325029/1‑149 (MQ=255)
                                                                                       cccGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAgcg                                                               >  6:100783/1‑149 (MQ=255)
                                                                                               ccACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCAcc                                                       <  8:123065/149‑1 (MQ=255)
                                                                                                    t‑tGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCTGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGAc                                                  >  2:235211/1‑149 (MQ=255)
                                                                                                    t‑tGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGAc                                                  >  1:226625/1‑149 (MQ=255)
                                                                                                             cacaTCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTg                                          <  1:135719/149‑1 (MQ=255)
                                                                                                              acaTCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTgg                                         >  2:153881/1‑149 (MQ=255)
                                                                                                                    tGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTg                                                                                        >  6:12727/1‑96 (MQ=255)
                                                                                                                       tCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTAcc                                >  2:9053/1‑149 (MQ=255)
                                                                                                                       tCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTAcc                                >  2:15192/1‑149 (MQ=255)
                                                                                                                           gggTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCCGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGAACAGCGCCTGCCCCTCGCCCAGCATAGGCCGGGTACCCTcc                            >  5:369242/1‑149 (MQ=255)
                                                                                                                           gggTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTcc                            >  1:363793/1‑149 (MQ=255)
                                                                                                                           gggTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTcc                            >  7:74742/1‑149 (MQ=255)
                                                                                                                            ggTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTccc                           <  4:119310/149‑1 (MQ=255)
                                                                                                                            ggTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTcc                            <  2:126486/148‑1 (MQ=255)
                                                                                                                                 ggAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCa                                                                  >  1:336182/1‑105 (MQ=255)
                                                                                                                                 ggAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCa                                                                  <  2:336182/105‑1 (MQ=255)
                                                                                                                                 ggAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGtt                      <  4:192263/149‑1 (MQ=255)
                                                                                                                                   aT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGcc                                                                                     >  6:315943/1‑84 (MQ=255)
                                                                                                                                   aT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGcc                                                                                     <  5:315943/84‑1 (MQ=255)
                                                                                                                                         gCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATca               >  4:119449/1‑149 (MQ=255)
                                                                                                                                          ccATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcac              >  1:233076/1‑149 (MQ=255)
                                                                                                                                           gttGGCGGTGCTCCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacc             >  4:124227/3‑149 (MQ=255)
                                                                                                                                            ttGGCGGTGCTCCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATcacca            >  2:3291/2‑149 (MQ=255)
                                                                                                                                                cGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGc         >  5:108332/1‑148 (MQ=255)
                                                                                                                                                     taGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCg   <  6:374657/148‑1 (MQ=255)
                                                                                                                                                     gAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTAccc    <  2:226625/148‑1 (MQ=255)
                                                                                                                                                     gAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTAccc    <  8:131836/148‑1 (MQ=255)
                                                                                                                                                       gCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATca               <  6:89092/135‑1 (MQ=255)
                                                                                                                                                       gCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATca               >  5:89092/1‑135 (MQ=255)
                                                                                                                                                       gCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTAccccg  >  1:334122/1‑146 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
AAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCC‑GAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGT‑TGAGAAACACATCCTGGTCATGGGTCGGGAT‑ACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTCGACCAGCGCCTGCACCTCGACCAGCATTGGCCGGGTACCCTCCCAGGTTGCCATCACCACGCTACCCGG  >  NC_002947/5269167‑5269462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑