Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,289,590	G→C	5.3%	L246V (CTC→GTC)	PP_4663 ←	transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,289,590	0	G	C	5.3%	244.4 / 2.2	75	L246V (CTC→GTC)	PP_4663	transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (40/31);  new base C (2/2);  total (42/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGCCC  >  NC_002947/5289443‑5289737                                                                                                                                                       |                                                                                                                                                       gCCGCGCGGGACGCGCACTCGGCCCTTCCGCCCAAGGCCGCCTTCTTCGAAATGCGCATGGACTACCTGGAGCGCGAGTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GG‑GGCCGCCGAgg                                                                                                                                                       <  6:106606/149‑1 (MQ=255)    gcgcTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGg‑‑‑gcg                                                                                                                                                 <  5:19427/149‑1 (MQ=255)    gcgcTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGGCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGg‑‑‑gcg                                                                                                                                                 <  1:57878/149‑1 (MQ=255)         ggACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAg                                                                                                                                            >  5:11281/1‑149 (MQ=255)         agagttGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTTATCAGCAG‑‑GT‑GGCCGCCCTCG‑‑‑GCGGCCac                                                                                                                                            <  1:320474/144‑2 (MQ=255)          gACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCATCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAgg                                                                                                                                           >  4:238722/1‑149 (MQ=255)            cGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGcc                                                                                                                                         <  4:155432/149‑1 (MQ=255)            cGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGcc                                                                                                                                         >  3:183875/1‑149 (MQ=255)             gTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGcc                                                                                                                                         >  4:76800/1‑148 (MQ=255)                  cTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCCGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAgg                                                                                                                                           >  1:259852/1‑141 (MQ=255)                  cTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCCGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAgg                                                                                                                                           >  1:264964/1‑141 (MQ=255)                  cTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCCGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAgg                                                                                                                                           <  2:259852/141‑1 (MQ=255)                  cTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCCGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAgg                                                                                                                                           <  2:264964/141‑1 (MQ=255)                   tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTACCCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGaa                                                                                                                                   >  4:68611/1‑148 (MQ=255)                   tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAg                                                                                                                                                        >  3:114262/1‑130 (MQ=255)                   tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAg                                                                                                                                                        <  4:114262/130‑1 (MQ=255)                   tCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGaa                                                                                                                                   >  5:179598/1‑148 (MQ=255)                              gCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCATCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCtt                                                                                                                       <  3:238722/149‑1 (MQ=255)                              gCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCtt                                                                                                                       <  7:150478/149‑1 (MQ=255)                                acaTGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTg                                                                                                                      >  3:142057/1‑148 (MQ=255)                                acaTGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTg                                                                                                                      >  4:243505/1‑148 (MQ=255)                                  aTGGCCCCCTTCTGCGACAAGCGCAGCGACTACCTGGAGCGCGATTTCGATATCCAGCGGGGCTACAGGCTCAGCGCTTCTTCCGATACCCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAAc                                                                                                                   <  8:63219/149‑1 (MQ=255)                                    ggCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAAcag                                                                                                                 >  1:95859/1‑149 (MQ=255)                                           ttcttcGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAgcgcg                                                                                                          >  6:7817/1‑149 (MQ=255)                                              ttcGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCt                                                                                                        >  3:271120/1‑148 (MQ=255)                                                               cTACCTGGAGCGCGATTTCGATATCCACCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCa                                                                                      >  5:119433/1‑149 (MQ=255)                                                                tACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCAc                                                                                     >  3:225999/1‑149 (MQ=255)                                                                tACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCAc                                                                                     >  5:103972/1‑149 (MQ=255)                                                                tACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCAc                                                                                     >  4:97799/1‑149 (MQ=255)                                                                  ccTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCCGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTg                                                                                   <  8:38141/149‑1 (MQ=255)                                                                   cTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCAc                                                                                     >  6:308255/1‑146 (MQ=255)                                                                   cTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCAc                                                                                     <  5:308255/146‑1 (MQ=255)                                                                          gcgATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGc                                                                             >  6:212740/1‑147 (MQ=255)                                                                             aTTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCa                                                                        >  8:240241/1‑149 (MQ=255)                                                                              tttCGATATCCCGCGGGTCGACATGCGCAGCGCTTCGTCCGCAAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATcc                                                                                                   <  4:115617/121‑1 (MQ=255)                                                                              tttCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATcc                                                                                                   >  3:115617/1‑121 (MQ=255)                                                                                 ctatatCCTCCGGGTGGACATTCTCAGCGCTTCTTCCCCTAAACGTTTTTCAGCAGCACTCGGCCGCCGCCGCCATCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCa                                                                           <  1:303092/146‑1 (MQ=255)                                                                                 cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGcc                                                                    >  6:380382/1‑149 (MQ=255)                                                                                 cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGc                                                                     <  4:225999/148‑1 (MQ=255)                                                                                 cGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGc                                                                     <  5:212740/148‑1 (MQ=255)                                                                                  gATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCg                                                                   <  7:240241/149‑1 (MQ=255)                                                                                    tatCCAGGGGGTCGACATGGTCTGCGCTTCGTCCGATAACGGTTGATCAGCTG‑‑GT‑GGCCGCCTTGG‑‑‑GCGGCCAGGCCGATGAAAAAGCTACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAg                                                                 <  6:119433/149‑1 (MQ=255)                                                                                       ccAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAAAGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCAGCCAGCATGCGGAGc                                                                >  3:197707/1‑147 (MQ=255)                                                                                        cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                                  >  4:168573/1‑144 (MQ=255)                                                                                        cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGa                                                                  <  3:168573/144‑1 (MQ=255)                                                                                        cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCaa                                                              <  3:97799/148‑1 (MQ=255)                                                                                        cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAg                                                             <  3:103471/149‑1 (MQ=255)                                                                                        cAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAg                                                             <  5:380382/149‑1 (MQ=255)                                                                                          gCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGc                                                           <  6:179598/149‑1 (MQ=255)                                                                                            gggTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCg                                                         <  3:243505/149‑1 (MQ=255)                                                                                            gggTCGACATCCACAGCGCGTCCTCCGCTACCCGTCGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCg                                                         <  4:271120/149‑1 (MQ=255)                                                                                              gTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGgc                                                       >  8:266786/1‑149 (MQ=255)                                                                                                      gCTCAGCGCTTCGTCCGCTAACCGTTGATCGGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                               >  5:287971/1‑149 (MQ=255)                                                                                                      gCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                               >  1:114422/1‑149 (MQ=255)                                                                                                      gCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACACTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCAt                                               >  5:129056/1‑149 (MQ=255)                                                                                                       cTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGc                                                                                        <  4:343783/107‑1 (MQ=255)                                                                                                       cTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGc                                                                                        >  3:343783/1‑107 (MQ=255)                                                                                                          aGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                            <  7:266786/148‑1 (MQ=255)                                                                                                           gcgcTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATcag                                            >  1:351408/1‑147 (MQ=255)                                                                                                                 cGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGAc                                     <  6:103972/148‑1 (MQ=255)                                                                                                                  gTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGc                                   >  6:236915/1‑149 (MQ=255)                                                                                                                   tCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcc                                  >  5:271201/1‑149 (MQ=255)                                                                                                                      gCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGccccc                               <  3:76800/149‑1 (MQ=255)                                                                                                                      gCTAACCGTTGATCAGCAG‑‑GT‑GGACGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGcccc                                >  7:119923/1‑148 (MQ=255)                                                                                                                        tAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCAGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAg                             >  5:227161/1‑149 (MQ=255)                                                                                                                            cGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGcc                         >  4:324396/1‑149 (MQ=255)                                                                                                                                  tCAGCAG‑‑GT‑GGACGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGcc                    <  8:119923/148‑1 (MQ=255)                                                                                                                                    agcag‑‑GT‑GGACGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGCCGCCAGCATCAGCAGCGACGCCCACAGCGCCTGGCCAcc                 >  6:218715/1‑149 (MQ=255)                                                                                                                                       ag‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAccc                >  8:330601/1‑147 (MQ=255)                                                                                                                                        ggcct‑GGCCGCCCTCG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCAccc                >  4:54189/5‑148 (MQ=255)                                                                                                                                        g‑‑gT‑GGCCGCCTAGG‑‑‑CCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAg             <  6:11281/149‑1 (MQ=255)                                                                                                                                        g‑‑gT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAg             <  5:7817/149‑1 (MQ=255)                                                                                                                                            t‑GGCCGCCCTCG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAgg            >  7:113480/1‑148 (MQ=255)                                                                                                                                              ggCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCa          >  7:30953/1‑149 (MQ=255)                                                                                                                                                  gccgAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCt      >  8:168057/1‑149 (MQ=255)                                                                                                                                                     gAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGcc   >  5:178141/1‑149 (MQ=255)                                                                                                                                                      aGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGccc  <  7:168057/149‑1 (MQ=255)                                                                                                                                                      aGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACACTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGccc  <  6:129056/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                       GCCGCGCTGGACGTGCACTCGGCCCTTCCGCACATGGCCGCCTTCTTCGAAAAGCGCATGGACTACCTGGAGCGCGATTTCGATATCCAGCGGGTCGACATGCTCAGCGCTTCGTCCGCTAACCGTTGATCAGCAG‑‑GT‑GGCCGCCGAGG‑‑‑GCGGCCAGGCCGATGAAAAAGCAACGCTTGAACAGCAGCGCGCTGATCCGCTGACGCAGCCACTGGCCCGCCAGCATGCCGAGCAAGGCCGGCGCCAGCATCAGCAGCGACGCCCCCAGCGCCTGGCCACCCAAGGCATCCTGCCC  >  NC_002947/5289443‑5289737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑