Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,297,191	A→C	8.7%	S58A (TCG→GCG)	TpbB ←	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,297,191	0	A	C	8.7%	201.6 / 9.9	69	S58A (TCG→GCG)	TpbB	diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base C (3/3);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

GTTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACG  >  NC_002947/5297047‑5297339                                                                                                                                                 |                                                                                                                                                      gTTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGACGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCgg                                                                                                                                                   <  8:293662/149‑1 (MQ=255)  ttGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCggg                                                                                                                                                  <  6:132473/149‑1 (MQ=255)     ccAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGa                                                                                                                                               <  4:82186/149‑1 (MQ=255)     ccAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGa                                                                                                                                               <  1:335429/149‑1 (MQ=255)         tGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCaa                                                                                                                                           <  4:335895/149‑1 (MQ=255)            gcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAACta                                                                                                                                        <  1:320123/149‑2 (MQ=255)            gcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAACta                                                                                                                                        <  7:148458/149‑2 (MQ=255)             ccAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTg                                                                                                                                        >  2:86384/1‑148 (MQ=255)                    cgcgaccTCGTTGTTGAACACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTa                                                                                                                                 <  6:261683/143‑1 (MQ=255)                       gCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGt                                                                                                                             >  8:45823/1‑149 (MQ=255)                               tgttgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATa                                                                                                                     <  7:103922/148‑1 (MQ=255)                                  tgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGaca                                                                                                                 >  2:346070/1‑149 (MQ=255)                                   gAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGacac                                                                                                                >  6:253167/1‑149 (MQ=255)                                    aaGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                               >  1:47104/1‑149 (MQ=255)                                         cTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAggg                                                                                                          >  8:272725/1‑149 (MQ=255)                                         cTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAggg                                                                                                          >  6:247304/1‑149 (MQ=255)                                                    ccACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGcc                                                                                                >  5:227597/1‑148 (MQ=255)                                                       cttcttCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGTGCTCGCCctgc                                                                                            <  5:253167/149‑1 (MQ=255)                                                         tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCcagcag                                                                                          >  3:228996/1‑149 (MQ=255)                                                           ttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAggg                                                                                        >  3:370268/1‑149 (MQ=255)                                                               gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                    <  3:193697/149‑1 (MQ=255)                                                               gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                    <  7:272725/149‑1 (MQ=255)                                                               gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                    >  2:237357/1‑149 (MQ=255)                                                               gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                    >  7:209236/1‑149 (MQ=255)                                                                tACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                   <  5:247304/149‑1 (MQ=255)                                                                  cTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGga                                                                                 <  2:365458/149‑1 (MQ=255)                                                                   tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                          >  4:232951/1‑92 (MQ=255)                                                                   tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                          <  3:232951/92‑1 (MQ=255)                                                                   tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGTGCACGCCCAGCAGGGTGAgg                                                                                   >  3:249867/1‑146 (MQ=255)                                                                     gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                               >  3:51775/1‑148 (MQ=255)                                                                     gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                               >  8:75347/1‑148 (MQ=255)                                                                     gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgag                                                                                <  1:237357/147‑1 (MQ=255)                                                                     gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGGTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                               >  8:271633/1‑148 (MQ=255)                                                                       aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAc                                                                            >  2:243396/1‑149 (MQ=255)                                                                         tCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAccc                                                                          <  6:227597/149‑1 (MQ=255)                                                                            aCGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATCCCCGc                                                                        >  2:57292/1‑148 (MQ=255)                                                                              gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                     <  4:51775/149‑1 (MQ=255)                                                                              gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                     >  3:254798/1‑149 (MQ=255)                                                                                 aGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                               <  1:26709/104‑1 (MQ=255)                                                                                 aGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                               >  2:26709/1‑104 (MQ=255)                                                                                      ttCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCAc                                                                                                   >  6:315929/1‑111 (MQ=255)                                                                                      ttCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCAc                                                                                                   <  5:315929/111‑1 (MQ=255)                                                                                          ttGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaag                                                         >  1:84892/1‑149 (MQ=255)                                                                                          ttGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaag                                                         <  5:5276/149‑1 (MQ=255)                                                                                              cggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGcaagcaag                                                     <  4:370268/149‑1 (MQ=255)                                                                                                     gcTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGATATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAACAGGGTGAGGGAGATACCCGCCAGGCCCGCGGGCAGCAAGGCGACGc                                              >  6:43019/1‑149 (MQ=255)                                                                                                      cTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCt                                             <  5:216156/149‑1 (MQ=255)                                                                                                         tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCATGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                          <  6:40686/149‑1 (MQ=255)                                                                                                         tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                           <  7:45823/148‑1 (MQ=255)                                                                                                         tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                           <  8:209236/148‑1 (MQ=255)                                                                                                         tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                          <  1:346070/149‑1 (MQ=255)                                                                                                           aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                        >  1:118927/1‑149 (MQ=255)                                                                                                             cGAACACCACAGCCGCGTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGGTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTgg                                      <  4:66694/149‑1 (MQ=255)                                                                                                                acacCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                           >  3:197069/1‑141 (MQ=255)                                                                                                                acacCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                           <  4:197069/141‑1 (MQ=255)                                                                                                                   ccacAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACgg                                <  2:84892/149‑1 (MQ=255)                                                                                                                    cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGc                               >  5:56986/1‑149 (MQ=255)                                                                                                                       aGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCa                            <  5:315508/149‑1 (MQ=255)                                                                                                                                tACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTAATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgc                   >  4:328890/1‑149 (MQ=255)                                                                                                                                    ctgcaGTTGATCGCCCGCTCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAg               >  5:327388/5‑149 (MQ=255)                                                                                                                                     tgtAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg              <  3:57447/149‑1 (MQ=255)                                                                                                                                     tgcaGTTGATCGCCCGCTCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg              >  3:253744/4‑149 (MQ=255)                                                                                                                                       tAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                    <  3:47155/109‑1 (MQ=255)                                                                                                                                       tAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                    >  4:47155/1‑109 (MQ=255)                                                                                                                                          ttGATCGCCCGCTCGATCAACTACAGGTTATGGTTGGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa                >  1:255217/1‑143 (MQ=255)                                                                                                                                          ttGATCGCCCGCTCGATCAACTACAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa                <  2:255217/142‑1 (MQ=255)                                                                                                                                            gATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc       >  3:3388/1‑149 (MQ=255)                                                                                                                                             aTCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgcg      <  2:118927/149‑1 (MQ=255)                                                                                                                                             aTCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc       <  8:207524/148‑1 (MQ=255)                                                                                                                                                 aGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACg  <  7:271633/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      GTTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACG  >  NC_002947/5297047‑5297339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑