Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,297,196	G→T	8.6%	A56D (GCC→GAC) ‡	TpbB ←	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,297,196	0	G	T	8.6%	213.8 / 8.2	70	A56D (GCC→GAC) ‡	TpbB	diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base T (3/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

TTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGT  >  NC_002947/5297048‑5297341                                                                                                                                                     |                                                                                                                                                   ttGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCggg                                                                                                                                                    <  6:132473/149‑1 (MQ=255)    ccAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGa                                                                                                                                                 <  4:82186/149‑1 (MQ=255)    ccAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGa                                                                                                                                                 <  1:335429/149‑1 (MQ=255)        tGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCaa                                                                                                                                             <  4:335895/149‑1 (MQ=255)           gcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAACta                                                                                                                                          <  1:320123/149‑2 (MQ=255)           gcCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGCCCGCTCGATCAACta                                                                                                                                          <  7:148458/149‑2 (MQ=255)            ccAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTg                                                                                                                                          >  2:86384/1‑148 (MQ=255)                   cgcgaccTCGTTGTTGAACACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTa                                                                                                                                   <  6:261683/143‑1 (MQ=255)                      gCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGt                                                                                                                               >  8:45823/1‑149 (MQ=255)                              tgttgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATa                                                                                                                       <  7:103922/148‑1 (MQ=255)                                 tgAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGaca                                                                                                                   >  2:346070/1‑149 (MQ=255)                                  gAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGacac                                                                                                                  >  6:253167/1‑149 (MQ=255)                                   aaGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                                 >  1:47104/1‑149 (MQ=255)                                        cTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAggg                                                                                                            >  6:247304/1‑149 (MQ=255)                                        cTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAggg                                                                                                            >  8:272725/1‑149 (MQ=255)                                                   ccACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGcc                                                                                                  >  5:227597/1‑148 (MQ=255)                                                      cttcttCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGTGCTCGCCctgc                                                                                              <  5:253167/149‑1 (MQ=255)                                                        tcttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCcagcag                                                                                            >  3:228996/1‑149 (MQ=255)                                                          ttcGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAggg                                                                                          >  3:370268/1‑149 (MQ=255)                                                              gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                      >  2:237357/1‑149 (MQ=255)                                                              gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                      >  7:209236/1‑149 (MQ=255)                                                              gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                      <  7:272725/149‑1 (MQ=255)                                                              gTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAg                                                                                      <  3:193697/149‑1 (MQ=255)                                                               tACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAgg                                                                                     <  5:247304/149‑1 (MQ=255)                                                                 cTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGga                                                                                   <  2:365458/149‑1 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                            >  4:232951/1‑92 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAAc                                                                                                                                            <  3:232951/92‑1 (MQ=255)                                                                  tGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGTGCACGCCCAGCAGGGTGAgg                                                                                     >  3:249867/1‑146 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                                 >  8:75347/1‑148 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                                 >  3:51775/1‑148 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgag                                                                                  <  1:237357/147‑1 (MQ=255)                                                                    gCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGGTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGgaga                                                                                 >  8:271633/1‑148 (MQ=255)                                                                      aaTCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAc                                                                              >  2:243396/1‑149 (MQ=255)                                                                        tCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATAccc                                                                            <  6:227597/149‑1 (MQ=255)                                                                           aCGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATCCCCGc                                                                          >  2:57292/1‑148 (MQ=255)                                                                             gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                       >  3:254798/1‑149 (MQ=255)                                                                             gCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAg                                                                       <  4:51775/149‑1 (MQ=255)                                                                                aGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                                 >  2:26709/1‑104 (MQ=255)                                                                                aGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACg                                                                                                                 <  1:26709/104‑1 (MQ=255)                                                                                     ttCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCAc                                                                                                     <  5:315929/111‑1 (MQ=255)                                                                                     ttCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCAc                                                                                                     >  6:315929/1‑111 (MQ=255)                                                                                         ttGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaag                                                           >  1:84892/1‑149 (MQ=255)                                                                                         ttGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGgcaag                                                           <  5:5276/149‑1 (MQ=255)                                                                                             cggcggcgCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGcaagcaag                                                       <  4:370268/149‑1 (MQ=255)                                                                                                    gcTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGATATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAACAGGGTGAGGGAGATACCCGCCAGGCCCGCGGGCAGCAAGGCGACGc                                                >  6:43019/1‑149 (MQ=255)                                                                                                     cTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCt                                               <  5:216156/149‑1 (MQ=255)                                                                                                        tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCATGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                            <  6:40686/149‑1 (MQ=255)                                                                                                        tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                             <  7:45823/148‑1 (MQ=255)                                                                                                        tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                             <  8:209236/148‑1 (MQ=255)                                                                                                        tCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAg                                            <  1:346070/149‑1 (MQ=255)                                                                                                          aTCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGt                                          >  1:118927/1‑149 (MQ=255)                                                                                                            cGAACACCACAGCCGCGTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGGTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTgg                                        <  4:66694/149‑1 (MQ=255)                                                                                                               acacCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                             <  4:197069/141‑1 (MQ=255)                                                                                                               acacCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGa                                             >  3:197069/1‑141 (MQ=255)                                                                                                                  ccacAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACgg                                  <  2:84892/149‑1 (MQ=255)                                                                                                                   cacaGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGc                                 >  5:56986/1‑149 (MQ=255)                                                                                                                      aGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCa                              <  5:315508/149‑1 (MQ=255)                                                                                                                               tACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTAATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAgc                     >  4:328890/1‑149 (MQ=255)                                                                                                                                   ctgcaGTTGATCGCCCGCTCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAg                 >  5:327388/5‑149 (MQ=255)                                                                                                                                    tgtAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg                <  3:57447/149‑1 (MQ=255)                                                                                                                                    tgcaGTTGATCGCCCGCTCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAgg                >  3:253744/4‑149 (MQ=255)                                                                                                                                      tAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                      >  4:47155/1‑109 (MQ=255)                                                                                                                                      tAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAgg                                                      <  3:47155/109‑1 (MQ=255)                                                                                                                                         ttGATCGCCCGCTCGATCAACTACAGGTTATGGTTGGGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa                  >  1:255217/1‑143 (MQ=255)                                                                                                                                         ttGATCGCCCGCTCGATCAACTACAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCa                  <  2:255217/142‑1 (MQ=255)                                                                                                                                           gATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc         >  3:3388/1‑149 (MQ=255)                                                                                                                                            aTCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgcg        <  2:118927/149‑1 (MQ=255)                                                                                                                                            aTCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGgc         <  8:207524/148‑1 (MQ=255)                                                                                                                                                aGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACg    <  7:271633/149‑1 (MQ=255)                                                                                                                                                  cGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACgg   >  6:86651/1‑148 (MQ=255)                                                                                                                                                  cGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGGCGGGCGCACGGt  <  7:75347/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTGCCAGTGCGCCAGTTGCTCGCCCTCGTTGTTGAAGACCTTGGCCTCGGCCACTTCTTCGGTACTGGCAATCAACGCCAGCGATTCGTTGGCGGCGGCGCTGTCATCGAACACCACAGCCGCTTCTACGGTGTAGTTGATCGAGCGGGCGATCAACTGCAGGTTATGGTT‑GGCATAGACACGCAGGGCGAGCACGCCCAGCAGGGTGAGGGAGATACCCGCCAGGCCAACGGCAAGCAAGGCGACGCTGAGGTGGCCACGGCCCAGCACCGAGCGCAGGGTCGGGCGCACGGT  >  NC_002947/5297048‑5297341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑