Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,356,023 G→A 5.8% V27V (GTC→GTT)  rbfA ← ribosome‑binding factor A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,356,0230GA 5.8% 210.6 / 2.4 69V27V (GTC→GTT) rbfAribosome‑binding factor A
Reads supporting (aligned to +/- strand):  ref base G (30/35);  new base A (2/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCTGGCGGCGCTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTACCT  >  NC_002947/5355876‑5356168
                                                                                                                                                   |                                                                                                                                                 
gCTGGCGGCGCTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGa                                                                                                                                                  <  4:228850/149‑1 (MQ=255)
        cgcTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGa                                                                                                                                          <  5:352341/149‑1 (MQ=255)
        cgcTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGa                                                                                                                                          >  1:46000/1‑149 (MQ=255)
         gcTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTAACTTCGCGAc                                                                                                                                         >  2:32946/1‑149 (MQ=255)
               cAATGCCTTGAGCGCCTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGTTGATGAACACATTTGCATGGCCCAGGTCAAGGCTCACGTCCACGGCGGTGATAGTCCCCAGACCGACACGCGCATCCTTGACTTCGCGACGGAt                                                                                                                                     >  6:206212/1‑147 (MQ=255)
                    ccTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGc                                                                                                                                 >  5:221037/1‑146 (MQ=255)
                    ccTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGc                                                                                                                                 <  6:221037/146‑1 (MQ=255)
                     cTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCgg                                                                                                                             >  5:115661/1‑149 (MQ=255)
                                 ctgcACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACg                                                                                                                                        <  3:51658/126‑1 (MQ=255)
                                 ctgcACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACg                                                                                                                                        >  4:51658/1‑126 (MQ=255)
                                 ctgcACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCgcgc                                                                                                                  <  1:228595/148‑1 (MQ=255)
                                    cACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGc                                                                                                               <  6:352561/148‑1 (MQ=255)
                                    cACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGc                                                                                                               <  6:115661/148‑1 (MQ=255)
                                    cACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTCATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCCCGCTGc                                                                                                               >  7:78714/1‑148 (MQ=255)
                                      cGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATc                                                                                                            >  2:136829/1‑149 (MQ=255)
                                      cGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATc                                                                                                            <  1:245411/149‑1 (MQ=255)
                                         cATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGa                                                                                                         <  4:368699/149‑1 (MQ=255)
                                          aTCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCAACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGAt                                                                                                        >  4:36313/1‑149 (MQ=255)
                                           tCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATc                                                                                                       >  6:377128/1‑149 (MQ=255)
                                             cGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGc                                                                                                     <  8:298413/149‑1 (MQ=255)
                                              ggggTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCGGCTCGGCCAGCTCGCGCTGCATCTGATCGc                                                                                                     >  1:98554/1‑148 (MQ=255)
                                                 gTTTCCTCGCCCATGACGGTGATGATCACGTTGGCATGGCGCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAAt                                                                                                 <  8:142538/149‑1 (MQ=255)
                                                      cTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGtt                                                                                            >  8:80227/1‑149 (MQ=255)
                                                      cTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGtt                                                                                            <  2:36685/149‑1 (MQ=255)
                                                                       tgaACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCt                                                                           >  4:208255/1‑149 (MQ=255)
                                                                          ccacCGTGGCATGGCCCAGGTCACGGCTGACGTCCACGGCTGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCttt                                                                         <  8:78714/147‑1 (MQ=255)
                                                                          actccTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGTTGATAGTCACCAGACCGACACGCGTATCCTTGACTTCGCGACTTATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCttt                                                                         <  5:206212/145‑1 (MQ=255)
                                                                          acacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTg                                                                        <  7:80227/149‑1 (MQ=255)
                                                                          acacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTg                                                                        <  7:19726/149‑1 (MQ=255)
                                                                           cacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACgg                                                                                    >  8:2585/1‑136 (MQ=255)
                                                                           cacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACgg                                                                                    <  7:2585/136‑1 (MQ=255)
                                                                           cacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTgg                                                                       <  4:177832/149‑1 (MQ=255)
                                                                           cacCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTgg                                                                       <  6:38416/149‑1 (MQ=255)
                                                                            acCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGc                                                                      >  7:157236/1‑149 (MQ=255)
                                                                             ccTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGc                                                                      >  3:30434/1‑148 (MQ=255)
                                                                              cTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATc                                                                                                       >  4:38136/1‑114 (MQ=255)
                                                                              cTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATc                                                                                                       <  3:38136/114‑1 (MQ=255)
                                                                              cTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTg                                                                                           >  6:193378/1‑126 (MQ=255)
                                                                              cTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTg                                                                                           <  5:193378/126‑1 (MQ=255)
                                                                              cTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCa                                                                    >  3:354579/1‑149 (MQ=255)
                                                                                 ggCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTc                                                                 >  4:61288/1‑149 (MQ=255)
                                                                                  gCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCt                                                                <  4:30434/149‑1 (MQ=255)
                                                                                        cccAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTAc                                                          <  3:208255/149‑1 (MQ=255)
                                                                                        cccAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGGTGGGTACGGCTATATTCTTTGGCCATTCTTGCTAc                                                          <  1:136829/149‑1 (MQ=255)
                                                                                                     tCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAg                                             >  2:220245/1‑149 (MQ=255)
                                                                                                       aCGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTAACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAg                                             <  1:32946/147‑1 (MQ=255)
                                                                                                           ccACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTAACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCa                                        >  5:94347/1‑148 (MQ=255)
                                                                                                            cACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTgg                                                                       >  5:314140/1‑116 (MQ=255)
                                                                                                            cACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTgg                                                                       <  6:314140/116‑1 (MQ=255)
                                                                                                             aCGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAAc                                     >  1:232854/1‑149 (MQ=255)
                                                                                                                 cggTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCCTGCTACCTGTAACTTAAAGCGGCAAACGccc                                 <  4:354579/149‑1 (MQ=255)
                                                                                                                    tGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGa                              <  8:157236/149‑1 (MQ=255)
                                                                                                                     gATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGa                              >  6:172911/1‑148 (MQ=255)
                                                                                                                     gATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGa                              >  3:1835/1‑148 (MQ=255)
                                                                                                                         gTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGa                          <  2:46000/148‑1 (MQ=255)
                                                                                                                          tCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTaa                                                    <  2:140229/121‑1 (MQ=255)
                                                                                                                          tCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTaa                                                    >  1:140229/1‑121 (MQ=255)
                                                                                                                             ccAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAAt                     <  6:306943/149‑1 (MQ=255)
                                                                                                                               aGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGc                   >  2:193381/1‑149 (MQ=255)
                                                                                                                                       acGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCgg           <  5:172911/149‑1 (MQ=255)
                                                                                                                                       acGCGGATCCTTAACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCGAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCgg           <  6:94347/149‑1 (MQ=255)
                                                                                                                                        cgcgGATCCTTGACTTCGCGAGGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGCGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCggg          <  6:289863/149‑1 (MQ=255)
                                                                                                                                        cgcgGATCCTTGACTTCGCGACGGATCGGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCggg          <  2:98554/149‑1 (MQ=255)
                                                                                                                                         gcgGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGgc         <  8:70055/149‑1 (MQ=255)
                                                                                                                                              tCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTAc    <  1:220245/149‑1 (MQ=255)
                                                                                                                                               ccTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTAcc   >  1:263713/1‑149 (MQ=255)
                                                                                                                                                cTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTACCt  >  5:17556/1‑149 (MQ=255)
                                                                                                                                                 ttGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTCCGGGTATATTCTTTGGCCATTCTTGATACGTGTACGTTAAAGCGGCAAACGCCCGGCAAGAGGAATGCGGGACCGGGCGCTAcgt  >  5:363900/1‑146 (MQ=255)
                                                                                                                                                 ttGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTACCt  >  8:238802/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCTGGCGGCGCTATTCAATGCCTTGAGCGACTGCTGCACGGCATCCGGGGTTTCCTCGCCCATGACGGTGATGAACACCTTGGCATGGCCCAGGTCACGGCTCACGTCCACGGCGGTGATAGTCACCAGACCGACACGCGGATCCTTGACTTCGCGACGGATCAGCTCGGCCAGCTCGCGCTGCATCTGATCGCCAATACGTTGGGTACGGCTATATTCTTTGGCCATTCTTGCTACCTGTAACTTAAAGCGGCAAACGCCCGGACAGACGAATGCCTGACCGGGCGCTACCT  >  NC_002947/5355876‑5356168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑