BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,361,071	A→T	5.4%	intergenic (‑78/+23)	PP_t49 ← / ← PP_t48	tRNA‑Met/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,361,071	0	A	T	5.4%	344.7 / 6.5	110	intergenic (‑78/+23)	PP_t49/PP_t48	tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (49/55); new base T (2/4); total (51/59)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 110 total aligned reads displayed.

GCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTT  >  NC_002947/5360928‑5361214

gCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCg                                                                                                                                            >  5:16810/1‑149 (MQ=255)

 cTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCgg                                                                                                                                           <  6:269318/149‑1 (MQ=255)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                          >  2:124416/1‑149 (MQ=255)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAATATAATACCGAACCCTGTTGAGGGTCAACTCCAACCATTCACCTACAAGAAAG‑CACGCTAAAGCgg                                                                                                                                           >  8:302378/1‑148 (MQ=37)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCCCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                          >  2:269783/1‑149 (MQ=255)

    gATTTGAACCAACGACCTTCGGGTTATGAGCCCTACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAATTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCgg                                                                                                                                           <  7:302378/146‑1 (MQ=255)

    gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGct                                                                                                                                        <  8:311889/149‑1 (MQ=255)

         gAACCAACGACCTTCGGGTTATGAGCCCTACGAGCTACCAAGCTGATCCACCCC‑‑‑‑CCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGTGTCAAATCCAATCATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCa                                                                                                                                    <  5:212553/148‑1 (MQ=39)

         gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTTTAGCGGGCtttct                                                                                                                                    <  2:248505/148‑2 (MQ=255)

          aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCtctc                                                                                                                                     >  3:200850/1‑146 (MQ=255)

             cAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttcttgtag                                                                                                                               <  5:372632/149‑7 (MQ=38)

             cAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                               >  4:245530/1‑149 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                          >  4:206069/1‑137 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                          <  3:206069/137‑1 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                              <  7:167604/149‑1 (MQ=255)

                cGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTaa                                                                                                                            <  5:78144/149‑1 (MQ=255)

                 gACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                           <  3:245530/149‑1 (MQ=255)

                 gACCTTCGGGGTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAACCTGGGGCAAAAGTATAAGACCGAACCCTGCTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                           <  3:275212/149‑1 (MQ=255)

                 gACCTTAGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCCTCATCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                               <  1:125747/149‑1 (MQ=38)

                      tCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATACAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGCGCTCTCAAGCTTTAAttctta                                                                                                                      >  8:200465/1‑145 (MQ=255)

                        gggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                    <  8:210402/149‑1 (MQ=255)

                          gTTATTATCCCGACTATCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAACGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCga                                                                                                                  <  8:174637/149‑1 (MQ=255)

                          gTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                    >  5:72805/1‑147 (MQ=255)

                              tGAGCCCGACGAGCTACCAAGCTGCCCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAg                                                                                                              <  1:269783/149‑1 (MQ=255)

                                aGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             <  8:317484/148‑1 (MQ=255)

                                 gCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGAACGAACCCTGTTGTGGGTCAAATCCAAACATTCACCTACAATTAAGCCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAggg                                                                                                            <  1:72044/149‑1 (MQ=255)

                                    cgacgaGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGCCCGAACCCTGTTGAGGGTCAAATCCAAACAATCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                        <  6:303714/149‑1 (MQ=255)

                                    cgacgaGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                        <  6:72805/149‑1 (MQ=255)

                                    cgacgaGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACt                                                                                                        <  5:146853/149‑1 (MQ=255)

                                         aGCTACCACGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                   >  5:83335/1‑149 (MQ=255)

                                         aGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                   <  5:70904/149‑1 (MQ=255)

                                         aGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAc                                                                                                   <  2:45656/149‑1 (MQ=255)

                                           cTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAACCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAccc                                                                                                 >  1:213502/1‑149 (MQ=255)

                                            tACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                    <  8:302821/129‑1 (MQ=255)

                                            tACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAcc                                                                                                                    >  7:302821/1‑129 (MQ=255)

                                            tACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAccc                                                                                                 >  3:57770/1‑148 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAAcccc                                                                                                <  6:16810/147‑1 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTa                                                                                              >  5:216042/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTa                                                                                              >  6:325797/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTa                                                                                              >  8:316164/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTa                                                                                              >  3:83958/1‑149 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             <  2:251351/133‑1 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAgg                                                                                                             >  1:251351/1‑133 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTa                                                                                              >  8:28543/1‑148 (MQ=255)

                                                  gctgctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACAcc                                                                                          <  3:367168/149‑1 (MQ=255)

                                                  gctgctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACAcc                                                                                          >  7:45075/1‑149 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                      >  6:60729/1‑149 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                      <  4:212803/149‑1 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                      <  5:385700/149‑1 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAc                                                                                                                     >  6:381007/1‑115 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTAc                                                                                                                     <  5:381007/115‑1 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                   >  7:119241/1‑149 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                   >  6:242223/1‑149 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                   <  4:83958/149‑1 (MQ=255)

                                                          aCCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGc                                                                                  <  7:316164/149‑1 (MQ=255)

                                                              c‑‑‑‑gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                              <  2:209276/149‑1 (MQ=255)

                                                                   gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAac                                                                             <  8:342627/149‑1 (MQ=255)

                                                                        tgtcgcggggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAaccac                                                                          <  4:270582/141‑1 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTg                                                                                                                         <  7:2096/100‑1 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTg                                                                                                                         >  8:2096/1‑100 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCt                                                                        >  2:96718/1‑149 (MQ=255)

                                                                               gggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACTACCTCAAGGt                                                                  <  4:62206/148‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                               >  4:156749/1‑149 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                               <  5:325797/149‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                               <  4:200850/149‑1 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCg                                                              >  1:94538/1‑149 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCg                                                              >  4:94889/1‑149 (MQ=255)

                                                                                   aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgt                                                             <  6:216042/149‑1 (MQ=255)

                                                                                   aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATCGGCACAACCACCTCAAGGTTGCgt                                                             >  5:24373/1‑149 (MQ=255)

                                                                                    aaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtg                                                            >  5:314240/1‑149 (MQ=255)

                                                                                     aaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAg                                                                                                                                  <  1:206586/78‑1 (MQ=255)

                                                                                     aaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGATAAAGCGGGCTCTCAAg                                                                                                                                  >  2:206586/1‑78 (MQ=255)

                                                                                      aGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTc                                                          <  1:65474/149‑1 (MQ=255)

                                                                                       gTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCt                                                         <  4:312341/149‑1 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACTCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                   >  3:322644/1‑149 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGcac                                                                                >  8:344240/1‑120 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGcac                                                                                <  7:344240/120‑1 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                   >  2:272903/1‑149 (MQ=255)

                                                                                                         ttGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCg                                       <  5:242223/149‑1 (MQ=255)

                                                                                                            aGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCa                                    <  6:314240/149‑1 (MQ=255)

                                                                                                               gTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAta                                 >  3:163567/1‑149 (MQ=255)

                                                                                                                  aaaTCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtacta                              <  7:28543/149‑1 (MQ=255)

                                                                                                                    aTCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactac                             >  3:16077/1‑148 (MQ=255)

                                                                                                                       cAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttc                                                                                                                                     >  8:351104/1‑41 (MQ=14)

                                                                                                                       cAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttc                                                                                                                                     <  7:351104/41‑1 (MQ=14)

                                                                                                                       cAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATactactact                         >  5:29216/1‑149 (MQ=255)

                                                                                                                         aaCATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGTTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTg                       <  1:124416/149‑1 (MQ=255)

                                                                                                                                aCCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCg                <  6:222895/149‑1 (MQ=255)

                                                                                                                                    aCAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAGCAATTCAACCACCTCGGCAATACTACTAATTGAAACCCGTTAc            >  7:286254/1‑149 (MQ=255)

                                                                                                                                     cAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt           >  7:199274/1‑149 (MQ=255)

                                                                                                                                      aaGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt          >  4:130143/1‑149 (MQ=255)

                                                                                                                                      aaGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt          >  6:86116/1‑149 (MQ=255)

                                                                                                                                        gAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt        <  4:163567/149‑1 (MQ=255)

                                                                                                                                        gAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt        >  8:345724/1‑149 (MQ=255)

                                                                                                                                        gAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTCGAAACCCGTTACTTCt        <  2:213502/149‑1 (MQ=255)

                                                                                                                                         aaaG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTg       <  8:119241/149‑1 (MQ=255)

                                                                                                                                            g‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctc    <  7:345724/149‑1 (MQ=255)

                                                                                                                                              cccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct   >  8:168567/1‑149 (MQ=255)

                                                                                                                                               ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGCGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct   >  2:171638/1‑148 (MQ=255)

                                                                                                                                               ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGCGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct   <  1:171638/148‑1 (MQ=255)

GCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTACCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTT  >  NC_002947/5360928‑5361214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`