BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,361,072	A→T	5.4%	intergenic (‑79/+22)	PP_t49 ← / ← PP_t48	tRNA‑Met/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,361,072	0	A	T	5.4%	351.7 / 4.8	110	intergenic (‑79/+22)	PP_t49/PP_t48	tRNA‑Met/tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (49/55); new base T (2/4); total (51/59)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 113 total aligned reads displayed.

GCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCG  >  NC_002947/5360928‑5361220

gCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCg                                                                                                                                                   >  5:16810/1‑149 (MQ=255)

 cTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCgg                                                                                                                                                  <  6:269318/149‑1 (MQ=255)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                                 >  2:124416/1‑149 (MQ=255)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAATATAATACCGAACCCTGTTGAGGGTCAACTCCAACCATTCACCTACAAGAAAG‑CACGCTAAAGCgg                                                                                                                                                  >  8:302378/1‑148 (MQ=37)

  tGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCCCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                                 >  2:269783/1‑149 (MQ=255)

    gATTTGAACCAACGACCTTCGGGTTATGAGCCCTACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAATTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCgg                                                                                                                                                  <  7:302378/146‑1 (MQ=255)

    gATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGct                                                                                                                                               <  8:311889/149‑1 (MQ=255)

         gAACCAACGACCTTCGGGTTATGAGCCCTACGAGCTACCAAGCTGATCCACCCC‑‑‑‑CCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGTGTCAAATCCAATCATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCa                                                                                                                                           <  5:212553/148‑1 (MQ=39)

         gAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTTTAGCGGGCtttct                                                                                                                                           <  2:248505/148‑2 (MQ=255)

          aaCCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCtctc                                                                                                                                            >  3:200850/1‑146 (MQ=255)

             cAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttcttgtag                                                                                                                                      <  5:372632/149‑7 (MQ=38)

             cAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                                      >  4:245530/1‑149 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                                 >  4:206069/1‑137 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCggg                                                                                                                                                 <  3:206069/137‑1 (MQ=255)

              aaCGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCttt                                                                                                                                     <  7:167604/149‑1 (MQ=255)

                cGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTaa                                                                                                                                   <  5:78144/149‑1 (MQ=255)

                 gACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                                  <  3:245530/149‑1 (MQ=255)

                 gACCTTCGGGGTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAACCTGGGGCAAAAGTATAAGACCGAACCCTGCTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAAt                                                                                                                                  <  3:275212/149‑1 (MQ=255)

                 gACCTTAGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCCCTCATCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCtt                                                                                                                                      <  1:125747/149‑1 (MQ=38)

                      tCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATACAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGCGCTCTCAAGCTTTAAttctta                                                                                                                             >  8:200465/1‑145 (MQ=255)

                        gggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑cc                                                                                                                          <  8:210402/149‑1 (MQ=255)

                        gggTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑c                                                                                                                           <  6:193513/148‑1 (MQ=255)

                          gTTATTATCCCGACTATCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAACGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCga                                                                                                                        <  8:174637/149‑1 (MQ=255)

                          gTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑cc                                                                                                                          >  5:72805/1‑147 (MQ=255)

                              tGAGCCCGACGAGCTACCAAGCTGCCCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAg                                                                                                                    <  1:269783/149‑1 (MQ=255)

                                aGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAgg                                                                                                                   <  8:317484/148‑1 (MQ=255)

                                 gCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGAACGAACCCTGTTGTGGGTCAAATCCAAACATTCACCTACAATTAAGCCCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAggg                                                                                                                  <  1:72044/149‑1 (MQ=255)

                                    cgacgaGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGCCCGAACCCTGTTGAGGGTCAAATCCAAACAATCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACt                                                                                                              <  6:303714/149‑1 (MQ=255)

                                    cgacgaGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACt                                                                                                              <  5:146853/149‑1 (MQ=255)

                                         aGCTACCACGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAc                                                                                                         >  5:83335/1‑149 (MQ=255)

                                         aGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAc                                                                                                         <  2:45656/149‑1 (MQ=255)

                                         aGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAc                                                                                                         <  5:70904/149‑1 (MQ=255)

                                           cTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAACCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAccc                                                                                                       >  1:213502/1‑149 (MQ=255)

                                            tACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑cc                                                                                                                          >  7:302821/1‑129 (MQ=255)

                                            tACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAccc                                                                                                       >  3:57770/1‑148 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAAcccc                                                                                                      <  6:16810/147‑1 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTa                                                                                                    >  3:83958/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTa                                                                                                    >  7:342627/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTa                                                                                                    >  8:316164/1‑149 (MQ=255)

                                              ccAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTa                                                                                                    >  6:325797/1‑149 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAgg                                                                                                                   <  2:251351/133‑1 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAgg                                                                                                                   >  1:251351/1‑133 (MQ=255)

                                               cAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTa                                                                                                    >  8:28543/1‑148 (MQ=255)

                                                  gctgctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACAcc                                                                                                <  3:367168/149‑1 (MQ=255)

                                                  gctgctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACAcc                                                                                                >  7:45075/1‑149 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                            >  6:60729/1‑149 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                            <  4:212803/149‑1 (MQ=255)

                                                      ctCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTAt                                                                                            <  5:385700/149‑1 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑c                                                                                                                           >  6:381007/1‑115 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                         <  4:83958/149‑1 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                         >  6:242223/1‑149 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATggg                                                                                         >  7:119241/1‑149 (MQ=255)

                                                         cACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTCGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATg                                                                                           <  6:83335/147‑1 (MQ=255)

                                                          aCCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGc                                                                                        <  7:316164/149‑1 (MQ=255)

                                                              c‑‑‑‑gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACaa                                                                                    <  2:209276/149‑1 (MQ=255)

                                                                   gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAac                                                                                   <  4:57770/149‑1 (MQ=255)

                                                                   gcgACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAac                                                                                   <  8:342627/149‑1 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTg                                                                                                                                <  7:2096/100‑1 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTg                                                                                                                                >  8:2096/1‑100 (MQ=255)

                                                                        aaaGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCt                                                                              >  2:96718/1‑149 (MQ=255)

                                                                               gggCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACTACCTCAAGGt                                                                        <  4:62206/148‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTTGTACCCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTg                                                                      <  1:96718/149‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                                     >  4:156749/1‑149 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                                     <  4:200850/149‑1 (MQ=255)

                                                                                 gCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGc                                                                     <  5:325797/149‑1 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCg                                                                    >  4:94889/1‑149 (MQ=255)

                                                                                  cAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCg                                                                    >  1:94538/1‑149 (MQ=255)

                                                                                   aaaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATCGGCACAACCACCTCAAGGTTGCgt                                                                   >  5:24373/1‑149 (MQ=255)

                                                                                    aaaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCgtg                                                                  >  5:314240/1‑149 (MQ=255)

                                                                                     aaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAg                                                                                                                                         <  1:206586/78‑1 (MQ=255)

                                                                                     aaGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGATAAAGCGGGCTCTCAAg                                                                                                                                         >  2:206586/1‑78 (MQ=255)

                                                                                       gTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCt                                                               <  4:312341/149‑1 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACTCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                         >  3:322644/1‑149 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGcac                                                                                      <  7:344240/120‑1 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGcac                                                                                      >  8:344240/1‑120 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                         >  5:293048/1‑149 (MQ=255)

                                                                                             gACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAAt                                                         >  2:272903/1‑149 (MQ=255)

                                                                                                         ttGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCg                                             <  5:242223/149‑1 (MQ=255)

                                                                                                            aGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCa                                          <  6:314240/149‑1 (MQ=255)

                                                                                                                  aaaTCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtacta                                    <  7:28543/149‑1 (MQ=255)

                                                                                                                    aTCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAAtactac                                   >  3:16077/1‑148 (MQ=255)

                                                                                                                       cAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttc                                                                                                                                            >  8:351104/1‑41 (MQ=14)

                                                                                                                       cAAACATTCACCTACAAGAGAG‑CCCGCTTTAGCGGGCtttc                                                                                                                                            <  7:351104/41‑1 (MQ=14)

                                                                                                                       cAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATactactact                               >  5:29216/1‑149 (MQ=255)

                                                                                                                         aaCATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGTTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTg                             <  1:124416/149‑1 (MQ=255)

                                                                                                                                aCCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCg                      <  6:222895/149‑1 (MQ=255)

                                                                                                                                     cAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACt                 >  7:199274/1‑149 (MQ=255)

                                                                                                                                     cAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCCTTACt                 >  8:274109/1‑149 (MQ=255)

                                                                                                                                      aaGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt                >  4:130143/1‑149 (MQ=255)

                                                                                                                                      aaGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACtt                >  6:86116/1‑149 (MQ=255)

                                                                                                                                        gAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt              <  4:163567/149‑1 (MQ=255)

                                                                                                                                        gAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCt              >  8:345724/1‑149 (MQ=255)

                                                                                                                                            g‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctc          <  7:345724/149‑1 (MQ=255)

                                                                                                                                              cccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct         >  8:168567/1‑149 (MQ=255)

                                                                                                                                               ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGCGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct         >  2:171638/1‑148 (MQ=255)

                                                                                                                                               ccGCTTTAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGCGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGctct         <  1:171638/148‑1 (MQ=255)

                                                                                                                                               ccGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCtt        <  2:94538/149‑1 (MQ=255)

                                                                                                                                                     aaGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                             <  2:135088/90‑1 (MQ=255)

                                                                                                                                                     aaGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTAc                                                             >  1:135088/1‑90 (MQ=255)

                                                                                                                                                     aaGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCg  <  5:60729/149‑1 (MQ=255)

GCTGGATTTGAACCAACGACCTTCGGGTTATGAGCCCGACGAGCTACCAAGCTGCTCCACCCC‑‑‑‑GCGACAAAGCTGGGGCAAAAGTATAAGACCGAACCCTGTTGAGGGTCAAATCCAAACATTCACCTACAAGAAAG‑CCCGCTAAAGCGGGCTCTCAAGCTTTAATTGGTA‑CCGAGAAGGGGACTCGAACCCCTACACCCTATGGGCACAACCACCTCAAGGTTGCGTGTCTACCAATTCCACCACCTCGGCAATACTACTACTTGAAACCCGTTACTTCTGCTCTTGAGCCG  >  NC_002947/5360928‑5361220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`