Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,381,267 T→C 5.4% intergenic (+32/+33) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,381,2670TC 5.4% 224.8 / 2.0 75intergenic (+32/+33)bamE/PP_4732outer membrane protein assembly factor BamE/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/37);  new base C (2/2);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

GCGCAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACA  >  NC_002947/5381119‑5381415
                                                                                                                                                    |                                                                                                                                                    
gcgcAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACt                                                                                                                                                      <  5:49660/149‑1 (MQ=255)
    aaCCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGt                                                                                                                                                  <  4:57371/149‑1 (MQ=255)
        tGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                              >  4:148278/1‑149 (MQ=255)
        tGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCgg                                                                                                                                              >  3:145347/1‑149 (MQ=255)
         gaagaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTctcgg                                                                                                                                             >  1:134249/1‑145 (MQ=255)
          aagaaAAACCGGCCAAGCCTTGTTCGACAGAAGATTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCggg                                                                                                                                             <  2:283123/148‑1 (MQ=255)
              acaaCCGGCCACGCCTGGTTCGACCGAAGAGGCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCCGATGCAAAAAAGCCCGGACTTGGTCCGGGCtttt                                                                                                                                        <  7:136117/147‑1 (MQ=255)
               aaaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCttttt                                                                                                                                       >  3:365536/1‑149 (MQ=255)
               aaaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCttttt                                                                                                                                       <  5:251646/149‑1 (MQ=255)
                    ggCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCTTTtttgcat                                                                                                                                  <  5:143542/149‑4 (MQ=255)
                      ccAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTgc                                                                                                                                >  1:351591/1‑149 (MQ=255)
                       cAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTgcg                                                                                                                               >  2:139321/1‑149 (MQ=255)
                        aaGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCg                                                                                                                                               >  7:320342/1‑132 (MQ=255)
                        aaGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCg                                                                                                                                               <  8:320342/132‑1 (MQ=255)
                        aaGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTgcgc                                                                                                                              <  3:180835/149‑1 (MQ=255)
                          gCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCt                                                                                                                            <  1:139321/149‑1 (MQ=255)
                               gTTGGACCGAAGAGTCCATCCAGCGCGAGCTCGACACCAACCAGACTACCCCGGACCCGACCCCGGCACCGCTGCAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGCACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATAc                                                                                                                       <  7:94014/149‑1 (MQ=255)
                               gTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGACCATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTCTTTGCGCCATATAc                                                                                                                       >  2:261490/1‑149 (MQ=255)
                                ttCCACCCAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACAACCCCGGTACCGACCCAGGCACCGCTGGAAACCCGCTAAGCGGGCACATCAGATGCAAAAAAGCCCGGACAGGGTCCGGGCATATTTGTTTGCGCCTTAAACg                                                                                                                      >  6:264934/1‑149 (MQ=255)
                                   gACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTc                                                                                                                    <  2:134249/148‑1 (MQ=255)
                                   gACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCa                                                                                                                   <  4:113282/149‑1 (MQ=255)
                                     ccGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAgg                                                                                                                 >  3:32349/1‑149 (MQ=255)
                                          gagTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCAtt                                                                                                             >  3:340070/1‑148 (MQ=255)
                                             tccatccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                         <  4:290988/149‑1 (MQ=255)
                                               catccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAtt                                                                                                        <  3:310737/148‑1 (MQ=255)
                                               catcaaGCGCGAGATCTACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTg                                                                                                       <  8:35645/149‑1 (MQ=255)
                                                   caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCtt                                                                                                    <  4:145347/148‑1 (MQ=255)
                                                   caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                                   <  3:162805/149‑1 (MQ=255)
                                                   caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                                   <  7:146937/149‑1 (MQ=255)
                                                   caGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTg                                                                                                   >  1:35795/1‑149 (MQ=255)
                                                     gcgcGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAATAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGc                                                                                                 <  1:261490/149‑1 (MQ=255)
                                                        cgAGAGCGACACCATCGAGTCTACCCCTGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGAAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACg                                                                                              <  7:162608/149‑1 (MQ=255)
                                                               gACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCTCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCa                                                                                        <  2:172665/148‑1 (MQ=255)
                                                                 cacCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGaa                                                                                     <  8:83901/149‑1 (MQ=255)
                                                                  acCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAAc                                                                                    <  2:351591/149‑1 (MQ=255)
                                                                      tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCAt                                                                                <  4:32349/149‑1 (MQ=255)
                                                                          gaCTACCCCGGTCCCGACCCCGGCACCGCTTGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAc                                                                            <  4:264691/149‑1 (MQ=255)
                                                                             tACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAg                                                                         >  8:66968/1‑149 (MQ=255)
                                                                                cccGGTCCCGACCCCTGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGTCAGAACGCATCGACCAGCGt                                                                      <  4:234173/149‑1 (MQ=255)
                                                                                cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCATTTGCTTGGCACGCTGGCAGAACGCATCGACCAGCg                                                                       >  3:208837/1‑148 (MQ=255)
                                                                                cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGcat                                                                      >  3:22653/1‑147 (MQ=255)
                                                                                cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCg                                                                       >  4:356685/1‑148 (MQ=255)
                                                                                 ccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTa                                                                     <  2:35795/149‑1 (MQ=255)
                                                                                  cGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATCCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTAt                                                                    >  2:176549/1‑149 (MQ=255)
                                                                                  cGGTCCCGACCCCGACACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTAt                                                                    >  1:230514/1‑149 (MQ=255)
                                                                                     tCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcg                                                                 >  8:324797/1‑149 (MQ=255)
                                                                                        cGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGc                                                                                             <  7:200068/118‑1 (MQ=255)
                                                                                        cGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGc                                                                                             >  8:200068/1‑118 (MQ=255)
                                                                                            cccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCt                                                           >  3:328746/1‑148 (MQ=255)
                                                                                              cGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGAt                                                        <  3:148278/149‑1 (MQ=255)
                                                                                              cGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCATATTCGCCGCCTGAt                                                        <  4:22653/149‑1 (MQ=255)
                                                                                                    cGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGaa                                                    >  6:350653/1‑147 (MQ=255)
                                                                                                      tgatgttcccGCTTAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGc                                                >  6:116020/8‑149 (MQ=255)
                                                                                                      cTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGAtt                                                       <  4:328715/142‑1 (MQ=255)
                                                                                                      cTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGAtt                                                       >  3:328715/1‑142 (MQ=255)
                                                                                                       gatgttcccGCTTAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACACATCGACCAGCGTATTCGCCGCCTGATTGAATAGCg                                               >  7:275946/7‑149 (MQ=255)
                                                                                                         gAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCggg                                             >  6:145829/1‑149 (MQ=255)
                                                                                                           ttcccGCTTAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGcc                                           <  5:116020/147‑1 (MQ=255)
                                                                                                              ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAtt                                                                                                        <  5:153002/85‑1 (MQ=255)
                                                                                                              ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAtt                                                                                                        >  6:153002/1‑85 (MQ=255)
                                                                                                              ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCaa                                        >  4:105464/1‑149 (MQ=255)
                                                                                                                gCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACg                                      >  8:225210/1‑149 (MQ=255)
                                                                                                                     gCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGgcg                                 <  3:356685/149‑1 (MQ=255)
                                                                                                                       gggAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGgcgcg                               >  7:143937/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGCCCGGCCCAAGTCCGGGCTTTTTTGTTTGCGGCTTATACGTCAGGGCTTCAATTGGTTGGCACGGTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGc                >  2:230/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGc                <  8:143937/149‑1 (MQ=255)
                                                                                                                                      aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGc                >  5:261769/1‑149 (MQ=255)
                                                                                                                                       aaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGCCCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCg               <  1:176549/149‑1 (MQ=255)
                                                                                                                                        aaaaGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGaa                                                                                     >  6:144529/1‑78 (MQ=37)
                                                                                                                                        aaaaGCCCGGACCAAGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGaa                                                                                     <  5:144529/78‑1 (MQ=37)
                                                                                                                                           agcgaggACTTGGTCCGGGCTTTTTTGTTAGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAAAGCATCGACCAGCGTTTTCGCCTCCTGATTGAATAGCGGGCCCAACGTCTCGCGCACAATCGGCCCGGCGTAAt           <  5:264934/144‑1 (MQ=255)
                                                                                                                                             cccGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATc          >  2:338087/1‑148 (MQ=255)
                                                                                                                                               cGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCg         >  5:351973/1‑147 (MQ=255)
                                                                                                                                               cGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGaa       <  5:350653/149‑1 (MQ=255)
                                                                                                                                                 gACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAGCAGCGTATTAGCCGCCAGTGTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAg      >  1:121672/1‑148 (MQ=255)
                                                                                                                                                   cTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGAc   <  2:230514/149‑1 (MQ=255)
                                                                                                                                                    ttGGTCCTGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACa  <  4:365536/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGCAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAAGGACA  >  NC_002947/5381119‑5381415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑