Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,388,233 A→T 5.8% E581D (GAA→GAT)  dld2 → D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,388,2330AT 5.8% 207.4 / 2.8 69E581D (GAA→GAT) dld2D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base T (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

CCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGCTAAAACCGCCGATT  >  NC_002947/5388085‑5388378
                                                                                                                                                    |                                                                                                                                                 
ccgccgACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGaa                                                                                                                                                   <  3:300057/149‑1 (MQ=255)
 cgccgACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAAc                                                                                                                                                  <  5:216819/149‑1 (MQ=255)
    cgACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGa                                                                                                                                               >  7:39903/1‑149 (MQ=255)
          aaTCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCaaa                                                                                                                                         <  7:298677/149‑1 (MQ=255)
          aaTCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCaaa                                                                                                                                         <  5:121601/149‑1 (MQ=255)
               tcgACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGAt                                                                                                                                              >  3:345644/1‑139 (MQ=255)
               tcgACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGAt                                                                                                                                              <  4:345644/139‑1 (MQ=255)
               tcgACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCtac                                                                                                                                    >  1:190557/1‑149 (MQ=255)
               tcgACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCtac                                                                                                                                    <  4:265253/149‑1 (MQ=255)
                  aCAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCtaccag                                                                                                                                 <  2:190557/149‑1 (MQ=255)
                    aaGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCtaccagta                                                                                                                               <  8:82668/149‑1 (MQ=255)
                      gTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATGCCCGTGAACTGATGCAAAGCtaccagtac                                                                                                                              >  1:165076/1‑148 (MQ=255)
                         cATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCtaccagtaccag                                                                                                                           <  7:104440/148‑1 (MQ=255)
                         cATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAgg                                                                                                                          >  2:276004/1‑149 (MQ=255)
                         cATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAgg                                                                                                                          <  3:345845/149‑1 (MQ=255)
                            cGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGaa                                                                                                                                                   <  7:15893/121‑1 (MQ=255)
                            cGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGaa                                                                                                                                                   >  8:15893/1‑121 (MQ=255)
                                  cGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAgg                                                                                                                          >  5:278836/1‑140 (MQ=255)
                                  cGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAgg                                                                                                                          <  6:278836/140‑1 (MQ=255)
                                  cGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGaca                                                                                                                 >  1:156090/1‑149 (MQ=255)
                                  cGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGaca                                                                                                                 >  1:109759/1‑149 (MQ=255)
                                      ttCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCa                                                                                                                       <  7:36911/139‑1 (MQ=255)
                                      ttCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCa                                                                                                                       >  8:36911/1‑139 (MQ=255)
                                         tGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTgcgc                                                                                                          >  2:32278/1‑149 (MQ=255)
                                           cGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTgcgc                                                                                                          <  1:276004/147‑1 (MQ=255)
                                                    gtgCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGacac                                                                                                                >  6:11751/1‑132 (MQ=255)
                                                    gtgCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGacac                                                                                                                <  5:11751/132‑1 (MQ=255)
                                                     tgCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGc                                                                                                >  1:167833/1‑147 (MQ=255)
                                                     tgCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGc                                                                                                <  2:167833/147‑1 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGGCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCtgt                                                                                            >  1:30866/1‑149 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGa                                                                                                                   >  3:68827/1‑126 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGa                                                                                                                   <  4:68827/126‑1 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCtgg                                                                                            >  7:97365/1‑148 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCAGCGACACCTGCGCCGCTACCGGCCtgt                                                                                            >  8:172333/1‑149 (MQ=255)
                                                       cccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATAGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTCAACTGATGCAAAGCTACCAGTACCAGCGCATCGACACCTGCGCCGCTACCGGCCtgt                                                                                            >  7:292448/1‑149 (MQ=255)
                                                        ccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGaa                                                                                                                                                   >  1:187941/1‑93 (MQ=255)
                                                        ccGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGaa                                                                                                                                                   <  2:187941/93‑1 (MQ=255)
                                                           tCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTgcgc                                                                                        <  2:156090/149‑1 (MQ=255)
                                                             cAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGa                                                                                                                                               <  6:67823/92‑1 (MQ=255)
                                                             cAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGa                                                                                                                                               >  5:67823/1‑92 (MQ=255)
                                                             cAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGccc                                                                                      >  6:291330/1‑149 (MQ=255)
                                                                  gCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAgcgc                                                                                 >  4:82725/1‑149 (MQ=255)
                                                                         ccTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCgg                                                                          >  4:299173/1‑149 (MQ=255)
                                                                          cTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGt                                                                         <  8:39903/149‑1 (MQ=255)
                                                                             aGCCCGCGCCAGCGCATCGTCATGGGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTc                                                                        <  3:332829/147‑1 (MQ=255)
                                                                                ccGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCAt                                                                   >  7:243179/1‑149 (MQ=255)
                                                                                 cgcgCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGTGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATc                                                                  <  5:260309/149‑1 (MQ=255)
                                                                                 cgcgCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCAt                                                                   >  7:206667/1‑148 (MQ=255)
                                                                                 cgcgCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATc                                                                  <  6:64707/149‑1 (MQ=255)
                                                                                     ccAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAaca                                                              <  4:360985/149‑1 (MQ=255)
                                                                                      cAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAacac                                                             <  7:168220/149‑1 (MQ=255)
                                                                                      cAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAacac                                                             <  2:109759/149‑1 (MQ=255)
                                                                                                  aTGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGt                                                 <  3:299173/149‑1 (MQ=255)
                                                                                                  aTGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGt                                                 <  2:174522/149‑1 (MQ=255)
                                                                                                     tgGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTgaa                                              <  4:82409/149‑1 (MQ=255)
                                                                                                       gCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTgaaga                                            <  7:298188/149‑1 (MQ=255)
                                                                                                       gCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTgaag                                             >  3:334651/1‑148 (MQ=255)
                                                                                                              aTCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTgcg                                     >  5:57915/1‑149 (MQ=255)
                                                                                                               tCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCACCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGACACTgcg                                     >  7:254351/1‑148 (MQ=255)
                                                                                                                ccAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTgcg                                     >  5:177054/1‑147 (MQ=255)
                                                                                                                   ggCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGc                                 >  3:108156/1‑148 (MQ=255)
                                                                                                                      cAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAgg                             >  2:110529/1‑149 (MQ=255)
                                                                                                                      cAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAgg                             >  4:58725/1‑149 (MQ=255)
                                                                                                                                gcTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCa                   <  6:177054/149‑1 (MQ=255)
                                                                                                                                gcTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCa                   <  4:108156/149‑1 (MQ=255)
                                                                                                                                gcTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCa                   >  2:95852/1‑149 (MQ=255)
                                                                                                                                    gCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGc                >  5:134808/1‑148 (MQ=255)
                                                                                                                                      aTCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGCTa              >  8:109148/1‑148 (MQ=255)
                                                                                                                                         gATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGCTaaaa           <  2:18340/148‑1 (MQ=255)
                                                                                                                                              ccGTGATCTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCAc                  >  3:282240/1‑136 (MQ=255)
                                                                                                                                              ccGTGATCTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCAc                  <  4:277394/136‑1 (MQ=255)
                                                                                                                                              ccGTGATCTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCAc                  <  4:282240/136‑1 (MQ=255)
                                                                                                                                              ccGTGATCTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCAc                  >  3:277394/1‑136 (MQ=255)
                                                                                                                                                 tGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGCTAAAACCGCCGAtt  >  4:310304/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGCCGACAAAATCGTCGACAAGTGCATCGAGTGCGGCTTCTGCGAACCGGTGTGCCCGTCCAAGGGCCTGACCCTCAGCCCGCGCCAGCGCATCGTCATGTGGCGTGACATCCAGGCCAAGCAACGCGCTGGCATCGATACCCGTGAACTGATGCAAAGCTACCAGTACCAGGGCATCGACACCTGCGCCGCTACCGGCCTGTGCGCCCAGCGCTGCCCGGTCGGCATCAACACCGGCGAGCTGGTGAAGAAACTGCGCAGCCAGGCCGCCGACCACGCTAAAACCGCCGATT  >  NC_002947/5388085‑5388378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑