Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,543,124 A→C 7.6% A45A (GCT→GCG)  rplI ← 50S ribosomal protein L9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,543,1240AC 7.6% 195.1 / 7.4 66A45A (GCT→GCG) rplI50S ribosomal protein L9
Reads supporting (aligned to +/- strand):  ref base A (33/28);  new base C (3/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

TCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACC  >  NC_002947/5542982‑5543269
                                                                                                                                              |                                                                                                                                                  
tCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAgg                                                                                                                                              >  7:244705/1‑149 (MQ=255)
   aaccgaacAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTg                                                                                                                                           >  2:353653/1‑149 (MQ=255)
         acAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGTTGGCCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGgcggcg                                                                                                                                     >  5:315948/1‑149 (MQ=255)
            gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTTGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                  <  7:349356/149‑1 (MQ=255)
            gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                  <  1:83558/149‑1 (MQ=255)
                 ccTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCAGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaag                                                                                                                              <  6:223735/148‑6 (MQ=255)
                  cTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACg                                                                                                                            >  3:65708/1‑149 (MQ=255)
                      gtcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTgg                                                                                                                        >  7:36486/1‑149 (MQ=255)
                       tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCATCGCGTCTTTCAGCCGAAGCTTTCTTGTAAGCGGATGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                       <  6:362469/149‑1 (MQ=255)
                       tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                       >  5:234038/1‑149 (MQ=255)
                         gCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCt                                                                                                                     >  3:131831/1‑149 (MQ=255)
                          ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCTATTTTTGTATATTGTCTTTGAGACGAAGGTTTTTTGTAATATGATGCTTTTTCTAGCTCTTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                    <  8:244705/149‑1 (MQ=255)
                          ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                    >  2:213188/1‑149 (MQ=255)
                            aGCGGTGGCAGTGATGGTCACTTCCAGCTCCGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCCTAGGCAACGGTGCCCTTg                                                                                                                   >  8:130128/1‑148 (MQ=255)
                                gTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGaa                                                                                                              >  5:27428/1‑149 (MQ=255)
                                 tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                             >  7:265156/1‑149 (MQ=255)
                                 tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                             >  3:142007/1‑149 (MQ=255)
                                 tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                             <  3:60481/149‑1 (MQ=255)
                                   gCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATgg                                                                                                           <  6:27428/149‑1 (MQ=255)
                                     aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                         >  7:322574/1‑149 (MQ=255)
                                     aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                         <  2:374556/149‑1 (MQ=255)
                                         aTGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgcagca                                                                                                      <  4:131831/148‑1 (MQ=255)
                                            gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAgg                                                                                                    >  2:65814/1‑147 (MQ=255)
                                            gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGaa                                                                                                  <  1:353653/149‑1 (MQ=255)
                                             tCACTTCCAGCTCGGCCAGTTGGGCAGCGCTGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAg                                                                                                 >  6:384495/1‑149 (MQ=255)
                                             tCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCAGAATGGCAGCAGGAAg                                                                                                 >  5:233857/1‑149 (MQ=255)
                                                    cAGCTCGGCCAGTTTTGCAGCGAGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGc                                                                                          <  6:234038/149‑1 (MQ=255)
                                                      gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                               >  5:381531/1‑106 (MQ=255)
                                                      gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                               <  6:381531/110‑5 (MQ=255)
                                                      gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                        >  4:142135/1‑149 (MQ=255)
                                                       cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                        >  2:199510/1‑148 (MQ=255)
                                                       cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                        >  4:294567/1‑148 (MQ=255)
                                                        tCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGt                                                                                       >  4:120268/1‑148 (MQ=255)
                                                          ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                    >  4:96890/1‑149 (MQ=255)
                                                          ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                    >  4:96887/1‑149 (MQ=255)
                                                             cAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAActtc                                                                                 <  8:265156/149‑4 (MQ=255)
                                                                   ggCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTaa                                                                          <  1:199510/149‑1 (MQ=255)
                                                                       gctcgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACttt                                                                      <  2:205511/146‑1 (MQ=255)
                                                                          cgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAc                                                                   <  8:36486/149‑1 (MQ=255)
                                                                           ggCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACt                                                                  <  3:294567/149‑1 (MQ=255)
                                                                            gCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACtt                                                                 <  3:246519/149‑1 (MQ=255)
                                                                             cTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTAGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCCGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACttt                                                                >  4:294731/1‑149 (MQ=255)
                                                                                 cAGCCGAAGCTTTATTCTCAGCTGCTGCTTTTTCGAGCGCTGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTc                                                             <  6:83352/148‑1 (MQ=255)
                                                                                  aGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTAACGGCC‑TAACCAAGCCTTACGTTTTACttttaggc                                                           >  3:249976/1‑144 (MQ=255)
                                                                                           tttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgc                                                  >  3:368103/1‑149 (MQ=255)
                                                                                            ttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGTAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                 >  4:138728/1‑149 (MQ=255)
                                                                                             tCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                 <  1:272817/148‑1 (MQ=255)
                                                                                                  tCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcccaggt                                            <  3:142135/148‑1 (MQ=255)
                                                                                                    aGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAg                                         >  1:333337/1‑149 (MQ=255)
                                                                                                     gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTTCTTTGTCGCCCAGGTTGCCCAGGTTAGc                                        <  1:213188/149‑1 (MQ=255)
                                                                                                     gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                        <  4:65708/149‑1 (MQ=255)
                                                                                                     gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                        <  4:195126/149‑1 (MQ=255)
                                                                                                      cGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                        <  5:384495/148‑1 (MQ=255)
                                                                                                         ctgctTTTTCCAGCTCGGCACGCCGCTCTTTGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGACGTTACGGCCGTAACC‑CGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACt                                    >  6:275999/1‑149 (MQ=255)
                                                                                                          tgctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtt                                   >  3:268903/1‑149 (MQ=255)
                                                                                                            ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                 <  8:322574/149‑1 (MQ=255)
                                                                                                            ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                 >  4:310509/1‑149 (MQ=255)
                                                                                                            ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACttt                                  <  7:211799/148‑1 (MQ=255)
                                                                                                             tttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                 >  1:173494/1‑148 (MQ=255)
                                                                                                              ttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTc                               >  4:172133/1‑149 (MQ=255)
                                                                                                                   cAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCcagc                           <  3:294731/148‑1 (MQ=255)
                                                                                                                         tgCACGACGATCTTCGAACGCAGCCATGTTGGCGGCGTTTGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAGTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGAt                      <  6:315948/146‑1 (MQ=255)
                                                                                                                                cgCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCAt             >  4:142497/1‑149 (MQ=255)
                                                                                                                                        caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa      >  2:58765/2‑148 (MQ=255)
                                                                                                                                        caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa      >  6:297298/2‑148 (MQ=255)
                                                                                                                                           cGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAAcc  <  1:65814/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACC  >  NC_002947/5542982‑5543269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑