Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,543,129	G→A	7.3%	L44L (CTG→TTG)	rplI ←	50S ribosomal protein L9

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,543,129	0	G	A	7.3%	198.8 / 4.5	69	L44L (CTG→TTG)	rplI	50S ribosomal protein L9
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base A (3/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

TCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTT  >  NC_002947/5542982‑5543273                                                                                                                                                    |                                                                                                                                                  tCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAgg                                                                                                                                                  >  7:244705/1‑149 (MQ=255)    aaccgaacAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTg                                                                                                                                               >  2:353653/1‑149 (MQ=255)          acAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGTTGGCCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGgcggcg                                                                                                                                         >  5:315948/1‑149 (MQ=255)             gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTTGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                      <  7:349356/149‑1 (MQ=255)             gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                      <  1:83558/149‑1 (MQ=255)                  ccTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCAGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaag                                                                                                                                  <  6:223735/148‑6 (MQ=255)                   cTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACg                                                                                                                                >  3:65708/1‑149 (MQ=255)                       gtcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTgg                                                                                                                            >  7:36486/1‑149 (MQ=255)                        tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCATCGCGTCTTTCAGCCGAAGCTTTCTTGTAAGCGGATGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                           <  6:362469/149‑1 (MQ=255)                        tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                           >  5:234038/1‑149 (MQ=255)                          gCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCt                                                                                                                         >  3:131831/1‑149 (MQ=255)                           ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCTATTTTTGTATATTGTCTTTGAGACGAAGGTTTTTTGTAATATGATGCTTTTTCTAGCTCTTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                        <  8:244705/149‑1 (MQ=255)                           ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                        >  2:213188/1‑149 (MQ=255)                             aGCGGTGGCAGTGATGGTCACTTCCAGCTCCGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCCTAGGCAACGGTGCCCTTg                                                                                                                       >  8:130128/1‑148 (MQ=255)                                 gTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGaa                                                                                                                  >  5:27428/1‑149 (MQ=255)                                  tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                 <  3:60481/149‑1 (MQ=255)                                  tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                 >  3:142007/1‑149 (MQ=255)                                  tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                 >  7:265156/1‑149 (MQ=255)                                    gCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATgg                                                                                                               <  6:27428/149‑1 (MQ=255)                                      aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                             <  2:374556/149‑1 (MQ=255)                                      aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                             >  7:322574/1‑149 (MQ=255)                                          aTGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgcagca                                                                                                          <  4:131831/148‑1 (MQ=255)                                             gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAgg                                                                                                        >  2:65814/1‑147 (MQ=255)                                             gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGaa                                                                                                      <  1:353653/149‑1 (MQ=255)                                              tCACTTCCAGCTCGGCCAGTTGGGCAGCGCTGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAg                                                                                                     >  6:384495/1‑149 (MQ=255)                                              tCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCAGAATGGCAGCAGGAAg                                                                                                     >  5:233857/1‑149 (MQ=255)                                                     cAGCTCGGCCAGTTTTGCAGCGAGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGc                                                                                              <  6:234038/149‑1 (MQ=255)                                                       gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                   >  5:381531/1‑106 (MQ=255)                                                       gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                   <  6:381531/110‑5 (MQ=255)                                                       gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                            >  4:142135/1‑149 (MQ=255)                                                        cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                            >  2:199510/1‑148 (MQ=255)                                                        cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                            >  4:294567/1‑148 (MQ=255)                                                         tCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGt                                                                                           >  4:120268/1‑148 (MQ=255)                                                           ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                        >  4:96887/1‑149 (MQ=255)                                                           ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                        >  4:96890/1‑149 (MQ=255)                                                              cAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAActtc                                                                                     <  8:265156/149‑4 (MQ=255)                                                                    ggCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTaa                                                                              <  1:199510/149‑1 (MQ=255)                                                                        gctcgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACttt                                                                          <  2:205511/146‑1 (MQ=255)                                                                           cgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAc                                                                       <  8:36486/149‑1 (MQ=255)                                                                            ggCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACt                                                                      <  3:294567/149‑1 (MQ=255)                                                                             gCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACtt                                                                     <  3:246519/149‑1 (MQ=255)                                                                              cTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTAGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCCGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACttt                                                                    >  4:294731/1‑149 (MQ=255)                                                                                  cAGCCGAAGCTTTATTCTCAGCTGCTGCTTTTTCGAGCGCTGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTc                                                                 <  6:83352/148‑1 (MQ=255)                                                                                   aGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTAACGGCC‑TAACCAAGCCTTACGTTTTACttttaggc                                                               >  3:249976/1‑144 (MQ=255)                                                                                            tttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgc                                                      >  3:368103/1‑149 (MQ=255)                                                                                             ttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGTAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                     >  4:138728/1‑149 (MQ=255)                                                                                              tCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                     <  1:272817/148‑1 (MQ=255)                                                                                                   tCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcccaggt                                                <  3:142135/148‑1 (MQ=255)                                                                                                     aGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAg                                             >  1:333337/1‑149 (MQ=255)                                                                                                      gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTTCTTTGTCGCCCAGGTTGCCCAGGTTAGc                                            <  1:213188/149‑1 (MQ=255)                                                                                                      gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                            <  4:65708/149‑1 (MQ=255)                                                                                                      gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                            <  4:195126/149‑1 (MQ=255)                                                                                                       cGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                            <  5:384495/148‑1 (MQ=255)                                                                                                          ctgctTTTTCCAGCTCGGCACGCCGCTCTTTGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGACGTTACGGCCGTAACC‑CGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACt                                        >  6:275999/1‑149 (MQ=255)                                                                                                           tgctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtt                                       >  3:268903/1‑149 (MQ=255)                                                                                                             ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                     >  4:310509/1‑149 (MQ=255)                                                                                                             ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                     <  8:322574/149‑1 (MQ=255)                                                                                                             ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACttt                                      <  7:211799/148‑1 (MQ=255)                                                                                                              tttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                     >  1:173494/1‑148 (MQ=255)                                                                                                               ttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTc                                   >  4:172133/1‑149 (MQ=255)                                                                                                                    cAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCcagc                               <  3:294731/148‑1 (MQ=255)                                                                                                                          tgCACGACGATCTTCGAACGCAGCCATGTTGGCGGCGTTTGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAGTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGAt                          <  6:315948/146‑1 (MQ=255)                                                                                                                                 cgCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCAt                 >  4:142497/1‑149 (MQ=255)                                                                                                                                         caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa          >  6:297298/2‑148 (MQ=255)                                                                                                                                         caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa          >  2:58765/2‑148 (MQ=255)                                                                                                                                            cGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAAcc      <  1:65814/149‑1 (MQ=255)                                                                                                                                                 ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                    >  5:342210/1‑130 (MQ=255)                                                                                                                                                 ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                    <  6:342210/130‑1 (MQ=255)                                                                                                                                                 ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt  >  4:131098/1‑148 (MQ=255)                                                                                                                                                 ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt  >  3:204502/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TCGAACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTT  >  NC_002947/5542982‑5543273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑