Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,543,131 T→C 7.2% N43S (AAC→AGC) ‡ rplI ← 50S ribosomal protein L9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,543,1310TC 7.2% 199.4 / 4.3 69N43S (AAC→AGC) ‡rplI50S ribosomal protein L9
Reads supporting (aligned to +/- strand):  ref base T (35/29);  new base C (3/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

AACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTT  >  NC_002947/5542985‑5543279
                                                                                                                                                  |                                                                                                                                                     
aaccgaacAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTg                                                                                                                                                     >  2:353653/1‑149 (MQ=255)
      acAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGTTGGCCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGgcggcg                                                                                                                                               >  5:315948/1‑149 (MQ=255)
         gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTTGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                            <  7:349356/149‑1 (MQ=255)
         gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                            <  1:83558/149‑1 (MQ=255)
              ccTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCAGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaag                                                                                                                                        <  6:223735/148‑6 (MQ=255)
               cTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACg                                                                                                                                      >  3:65708/1‑149 (MQ=255)
                   gtcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTgg                                                                                                                                  >  7:36486/1‑149 (MQ=255)
                    tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCATCGCGTCTTTCAGCCGAAGCTTTCTTGTAAGCGGATGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                                 <  6:362469/149‑1 (MQ=255)
                    tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                                 >  5:234038/1‑149 (MQ=255)
                      gCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCt                                                                                                                               >  3:131831/1‑149 (MQ=255)
                       ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCTATTTTTGTATATTGTCTTTGAGACGAAGGTTTTTTGTAATATGATGCTTTTTCTAGCTCTTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                              <  8:244705/149‑1 (MQ=255)
                       ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                              >  2:213188/1‑149 (MQ=255)
                         aGCGGTGGCAGTGATGGTCACTTCCAGCTCCGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCCTAGGCAACGGTGCCCTTg                                                                                                                             >  8:130128/1‑148 (MQ=255)
                             gTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGaa                                                                                                                        >  5:27428/1‑149 (MQ=255)
                              tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                       >  7:265156/1‑149 (MQ=255)
                              tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                       >  3:142007/1‑149 (MQ=255)
                              tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                       <  3:60481/149‑1 (MQ=255)
                                gCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATgg                                                                                                                     <  6:27428/149‑1 (MQ=255)
                                  aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                                   >  7:322574/1‑149 (MQ=255)
                                  aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                                   <  2:374556/149‑1 (MQ=255)
                                      aTGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgcagca                                                                                                                <  4:131831/148‑1 (MQ=255)
                                         gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAgg                                                                                                              >  2:65814/1‑147 (MQ=255)
                                         gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGaa                                                                                                            <  1:353653/149‑1 (MQ=255)
                                          tCACTTCCAGCTCGGCCAGTTGGGCAGCGCTGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAg                                                                                                           >  6:384495/1‑149 (MQ=255)
                                          tCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCAGAATGGCAGCAGGAAg                                                                                                           >  5:233857/1‑149 (MQ=255)
                                                 cAGCTCGGCCAGTTTTGCAGCGAGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGc                                                                                                    <  6:234038/149‑1 (MQ=255)
                                                   gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                         <  6:381531/110‑5 (MQ=255)
                                                   gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                         >  5:381531/1‑106 (MQ=255)
                                                   gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                  >  4:142135/1‑149 (MQ=255)
                                                    cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                  >  2:199510/1‑148 (MQ=255)
                                                    cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                  >  4:294567/1‑148 (MQ=255)
                                                     tCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGt                                                                                                 >  4:120268/1‑148 (MQ=255)
                                                       ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                              >  4:96887/1‑149 (MQ=255)
                                                       ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                              >  4:96890/1‑149 (MQ=255)
                                                          cAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAActtc                                                                                           <  8:265156/149‑4 (MQ=255)
                                                                ggCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTaa                                                                                    <  1:199510/149‑1 (MQ=255)
                                                                    gctcgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACttt                                                                                <  2:205511/146‑1 (MQ=255)
                                                                       cgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAc                                                                             <  8:36486/149‑1 (MQ=255)
                                                                        ggCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACt                                                                            <  3:294567/149‑1 (MQ=255)
                                                                         gCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACtt                                                                           <  3:246519/149‑1 (MQ=255)
                                                                          cTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTAGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCCGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACttt                                                                          >  4:294731/1‑149 (MQ=255)
                                                                              cAGCCGAAGCTTTATTCTCAGCTGCTGCTTTTTCGAGCGCTGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTc                                                                       <  6:83352/148‑1 (MQ=255)
                                                                               aGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTAACGGCC‑TAACCAAGCCTTACGTTTTACttttaggc                                                                     >  3:249976/1‑144 (MQ=255)
                                                                                        tttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgc                                                            >  3:368103/1‑149 (MQ=255)
                                                                                         ttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGTAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                           >  4:138728/1‑149 (MQ=255)
                                                                                          tCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                           <  1:272817/148‑1 (MQ=255)
                                                                                               tCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcccaggt                                                      <  3:142135/148‑1 (MQ=255)
                                                                                                 aGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAg                                                   >  1:333337/1‑149 (MQ=255)
                                                                                                  gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTTCTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                  <  1:213188/149‑1 (MQ=255)
                                                                                                  gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                  <  4:65708/149‑1 (MQ=255)
                                                                                                  gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                  <  4:195126/149‑1 (MQ=255)
                                                                                                   cGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                  <  5:384495/148‑1 (MQ=255)
                                                                                                      ctgctTTTTCCAGCTCGGCACGCCGCTCTTTGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGACGTTACGGCCGTAACC‑CGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACt                                              >  6:275999/1‑149 (MQ=255)
                                                                                                       tgctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtt                                             >  3:268903/1‑149 (MQ=255)
                                                                                                         ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                           <  8:322574/149‑1 (MQ=255)
                                                                                                         ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                           >  4:310509/1‑149 (MQ=255)
                                                                                                         ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACttt                                            <  7:211799/148‑1 (MQ=255)
                                                                                                          tttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                           >  1:173494/1‑148 (MQ=255)
                                                                                                           ttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTc                                         >  4:172133/1‑149 (MQ=255)
                                                                                                                cAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCcagc                                     <  3:294731/148‑1 (MQ=255)
                                                                                                                      tgCACGACGATCTTCGAACGCAGCCATGTTGGCGGCGTTTGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAGTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGAt                                <  6:315948/146‑1 (MQ=255)
                                                                                                                             cgCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCAt                       >  4:142497/1‑149 (MQ=255)
                                                                                                                                     caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa                >  6:297298/2‑148 (MQ=255)
                                                                                                                                     caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa                >  2:58765/2‑148 (MQ=255)
                                                                                                                                        cGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAAcc            <  1:65814/149‑1 (MQ=255)
                                                                                                                                             ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                          <  6:342210/130‑1 (MQ=255)
                                                                                                                                             ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                          >  5:342210/1‑130 (MQ=255)
                                                                                                                                             ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt        >  3:204502/1‑148 (MQ=255)
                                                                                                                                             ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt        >  4:131098/1‑148 (MQ=255)
                                                                                                                                                  ttGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCGAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACttt  <  3:142497/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AACCGAACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTT  >  NC_002947/5542985‑5543279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑