Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,543,136 G→T 7.2% A41A (GCC→GCA)  rplI ← 50S ribosomal protein L9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,543,1360GT 7.2% 205.9 / 4.4 68A41A (GCC→GCA) rplI50S ribosomal protein L9
Reads supporting (aligned to +/- strand):  ref base G (32/30);  new base T (3/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

ACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTTTAAC  >  NC_002947/5542991‑5543283
                                                                                                                                                 |                                                                                                                                                    
acAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGTTGGCCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGgcggcg                                                                                                                                                   >  5:315948/1‑149 (MQ=255)
   gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTTGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                                <  7:349356/149‑1 (MQ=255)
   gCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTg                                                                                                                                                <  1:83558/149‑1 (MQ=255)
        ccTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCAGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaag                                                                                                                                            <  6:223735/148‑6 (MQ=255)
         cTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACg                                                                                                                                          >  3:65708/1‑149 (MQ=255)
             gtcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTgg                                                                                                                                      >  7:36486/1‑149 (MQ=255)
              tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCATCGCGTCTTTCAGCCGAAGCTTTCTTGTAAGCGGATGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                                     <  6:362469/149‑1 (MQ=255)
              tcgCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGc                                                                                                                                     >  5:234038/1‑149 (MQ=255)
                gCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCt                                                                                                                                   >  3:131831/1‑149 (MQ=255)
                 ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCTATTTTTGTATATTGTCTTTGAGACGAAGGTTTTTTGTAATATGATGCTTTTTCTAGCTCTTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                                  <  8:244705/149‑1 (MQ=255)
                 ccAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCtt                                                                                                                                  >  2:213188/1‑149 (MQ=255)
                   aGCGGTGGCAGTGATGGTCACTTCCAGCTCCGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCCTAGGCAACGGTGCCCTTg                                                                                                                                 >  8:130128/1‑148 (MQ=255)
                       gTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGaa                                                                                                                            >  5:27428/1‑149 (MQ=255)
                        tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                           >  3:142007/1‑149 (MQ=255)
                        tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                           >  7:265156/1‑149 (MQ=255)
                        tGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAAt                                                                                                                           <  3:60481/149‑1 (MQ=255)
                          gCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGTCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATgg                                                                                                                         <  6:27428/149‑1 (MQ=255)
                            aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                                       <  2:374556/149‑1 (MQ=255)
                            aGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgca                                                                                                                       >  7:322574/1‑149 (MQ=255)
                                aTGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGgcagca                                                                                                                    <  4:131831/148‑1 (MQ=255)
                                   gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAgg                                                                                                                  >  2:65814/1‑147 (MQ=255)
                                   gTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGaa                                                                                                                <  1:353653/149‑1 (MQ=255)
                                    tCACTTCCAGCTCGGCCAGTTGGGCAGCGCTGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAg                                                                                                               >  6:384495/1‑149 (MQ=255)
                                    tCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCAGAATGGCAGCAGGAAg                                                                                                               >  5:233857/1‑149 (MQ=255)
                                           cAGCTCGGCCAGTTTTGCAGCGAGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCAAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGc                                                                                                        <  6:234038/149‑1 (MQ=255)
                                             gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                             <  6:381531/110‑5 (MQ=255)
                                             gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCCGCCAACCTGGCTGCGttcgaa                                                                                                                                             >  5:381531/1‑106 (MQ=255)
                                             gCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                      >  4:142135/1‑149 (MQ=255)
                                              cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                      >  4:294567/1‑148 (MQ=255)
                                              cTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCg                                                                                                      >  2:199510/1‑148 (MQ=255)
                                               tCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGt                                                                                                     >  4:120268/1‑148 (MQ=255)
                                                 ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                                  >  4:96890/1‑149 (MQ=255)
                                                 ggCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAAc                                                                                                  >  4:96887/1‑149 (MQ=255)
                                                    cAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAActtc                                                                                               <  8:265156/149‑4 (MQ=255)
                                                          ggCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTaa                                                                                        <  1:199510/149‑1 (MQ=255)
                                                              gctcgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACttt                                                                                    <  2:205511/146‑1 (MQ=255)
                                                                 cgGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAc                                                                                 <  8:36486/149‑1 (MQ=255)
                                                                  ggCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACt                                                                                <  3:294567/149‑1 (MQ=255)
                                                                   gCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACtt                                                                               <  3:246519/149‑1 (MQ=255)
                                                                    cTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTAGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCCGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACttt                                                                              >  4:294731/1‑149 (MQ=255)
                                                                        cAGCCGAAGCTTTATTCTCAGCTGCTGCTTTTTCGAGCGCTGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTc                                                                           <  6:83352/148‑1 (MQ=255)
                                                                         aGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTAACGGCC‑TAACCAAGCCTTACGTTTTACttttaggc                                                                         >  3:249976/1‑144 (MQ=255)
                                                                                  tttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgc                                                                >  3:368103/1‑149 (MQ=255)
                                                                                   ttCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGTAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                               >  4:138728/1‑149 (MQ=255)
                                                                                    tCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcc                                                               <  1:272817/148‑1 (MQ=255)
                                                                                         tCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCgcccaggttgcccaggt                                                          <  3:142135/148‑1 (MQ=255)
                                                                                           aGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAg                                                       >  1:333337/1‑149 (MQ=255)
                                                                                            gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTTCTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                      <  1:213188/149‑1 (MQ=255)
                                                                                            gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                      <  4:195126/149‑1 (MQ=255)
                                                                                            gCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                      <  4:65708/149‑1 (MQ=255)
                                                                                             cGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGc                                                      <  5:384495/148‑1 (MQ=255)
                                                                                                ctgctTTTTCCAGCTCGGCACGCCGCTCTTTGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGACGTTACGGCCGTAACC‑CGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACt                                                  >  6:275999/1‑149 (MQ=255)
                                                                                                 tgctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtt                                                 >  3:268903/1‑149 (MQ=255)
                                                                                                   ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                               <  8:322574/149‑1 (MQ=255)
                                                                                                   ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                               >  4:310509/1‑149 (MQ=255)
                                                                                                   ctTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACttt                                                <  7:211799/148‑1 (MQ=255)
                                                                                                    tttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACtttt                                               >  1:173494/1‑148 (MQ=255)
                                                                                                     ttttCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTc                                             >  4:172133/1‑149 (MQ=255)
                                                                                                          cAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCcagc                                         <  3:294731/148‑1 (MQ=255)
                                                                                                                tgCACGACGATCTTCGAACGCAGCCATGTTGGCGGCGTTTGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTAGTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGAt                                    <  6:315948/146‑1 (MQ=255)
                                                                                                                       cgCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCAt                           >  4:142497/1‑149 (MQ=255)
                                                                                                                               caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa                    >  6:297298/2‑148 (MQ=255)
                                                                                                                               caaCGCCGCCAACCTGGCTGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTaa                    >  2:58765/2‑148 (MQ=255)
                                                                                                                                  cGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAAcc                <  1:65814/149‑1 (MQ=255)
                                                                                                                                       ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                              <  6:342210/130‑1 (MQ=255)
                                                                                                                                       ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTc                              >  5:342210/1‑130 (MQ=255)
                                                                                                                                       ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt            >  3:204502/1‑148 (MQ=255)
                                                                                                                                       ccAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCtt            >  4:131098/1‑148 (MQ=255)
                                                                                                                                            ttGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCGAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACttt      <  3:142497/149‑1 (MQ=255)
                                                                                                                                               gcggcgTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTa                                                        <  8:121230/96‑1 (MQ=255)
                                                                                                                                               gcggcgTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTa                                                        >  7:121230/1‑96 (MQ=255)
                                                                                                                                               gcggcgTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTTTaa   >  4:315250/1‑149 (MQ=255)
                                                                                                                                               gcggagTTGGCAACTGTGGCCTTGCCGAATGGCAGCAGGAAGGTAGGTCCTTAACC‑AGCCTTAACTATTAGTTTGTCGCCCAGGTTTCCCAGGTTAGAGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTTTa    <  7:130128/148‑1 (MQ=255)
                                                                                                                                                cggcgTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTTTAAc  <  3:172133/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACAGCTTGCCTTCGTCGCCAGCGGTGGCAGTGATGGTCACTTCCAGCTCGGCCAGTTGGGCAGCGCGGCTTTCAGCCGAAGCTTTCTTGTCAGCGGCTGCTTTTTCCAGCTCGGCACGACGCTCTTCGAACGCAGCCAGGTTGGCGGCGTTGGCAACGGTGGCCTTGCCGAATGGCAGCAGGAAGTTACGGCCGTAACC‑AGCCTTAACTTTTACTTTGTCGCCCAGGTTGCCCAGGTTAGCGACTTTTTCCAGCAGGATCAGTTCCATTTGGTAAAACCTCTTAACTTTTAAC  >  NC_002947/5542991‑5543283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑