| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,555,648 | T→A | 10.7% | intergenic (‑124/+47) | PP_4887 ← / ← PP_4888 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,555,648 | 0 | T | A | 10.7% | 111.2 / 5.2 | 47 | intergenic (‑124/+47) | PP_4887/PP_4888 | hypothetical protein/methyl‑accepting chemotaxis transducer |
| Reads supporting (aligned to +/- strand): ref base T (15/26); new base A (2/3); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01 | |||||||||||
GGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGAGCGTT > NC_002947/5555513‑5555795 | gacatgagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggcccCCGTCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCATAGCAgcccc < 1:279606‑M2/50‑2 (MQ=255)gacatgagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggcccCCGTCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCATAGCAgcccc < 1:284322‑M2/50‑2 (MQ=255) catgagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCATAgcagccccagg < 6:22320‑M2/53‑5 (MQ=255) tgagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTcgc < 2:30669‑M2/55‑1 (MQ=255) gagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtagcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTcgcg > 7:339884‑M2/94‑149 (MQ=255) gagcaactccttttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtagcgaaagggccgcaaagcggccCCGGGCATTTGCGCGGGTTTGGAAAAAACCAGGGGCGGCGATGCAGCCCGTTcgcg > 3:264120‑M2/94‑149 (MQ=255) cTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTTTAGCCAAATGGCCCCATAGCGGCCCCGGGAATTTTCGCGGGTTTTCCACCAACCAGGGGCGGGTATGCCGCCCGTTCGCGGCACACggg > 5:202133/1‑148 (MQ=255) ttgtcatgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGccc < 7:70982‑M2/67‑1 (MQ=255) cctgtgtcgccgacagcatagcaacaccgctcaggtgtgggagcggccttgtggcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACaaa < 3:40526‑M2/72‑1 (MQ=255) gacagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAg < 5:210647‑M2/83‑1 (MQ=255) acagcatagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAgg < 7:241768‑M2/84‑1 (MQ=255) catagcaacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCtt > 2:45222‑M2/62‑148 (MQ=255) aacaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGcc‑c < 4:347180‑M2/94‑1 (MQ=255) acaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTc < 5:31993‑M2/53‑1 (MQ=255) acaccgctcaggtgtgggagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTc > 6:31993‑M2/55‑107 (MQ=255) gagcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTgg < 3:309942‑M2/112‑1 (MQ=255) gcggccttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGTAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGgcg < 1:45222‑M2/114‑1 (MQ=255) cggccttgtgccgctaacgggccgcatcgcgcccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGgcgc < 5:250424‑M2/115‑1 (MQ=255) cttgtgtcgcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAg < 4:264120‑M2/119‑1 (MQ=255) gcgaaagggccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAgccg > 6:21730‑M2/23‑149 (MQ=255) ccgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGatcat > 6:254020‑M2/14‑149 (MQ=255) cgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGatcatc < 5:216504‑M2/137‑1 (MQ=255) cgcatagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGatcatc < 7:322852‑M2/137‑1 (MQ=255) atagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAg < 6:202133‑M2/140‑1 (MQ=255) tagcggccCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGc < 8:339884‑M2/141‑1 (MQ=255) ccGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAcc < 4:14886/144‑1 (MQ=255) ccGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCAcc > 3:14886/1‑144 (MQ=255) cGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGCCGCGCACATGAGCCGCCCGATCATCAAGCACCTTGCt > 3:144803/1‑148 (MQ=255) ggCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTAc < 5:21730/149‑1 (MQ=255) ggCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTAc > 7:311246/1‑149 (MQ=255) gCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTg < 4:200304/108‑1 (MQ=255) gCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTg > 3:200304/1‑108 (MQ=255) tCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCg < 7:188837/148‑1 (MQ=255) ccGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTgg > 2:273803/1‑144 (MQ=255) ccGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTgg < 1:273803/144‑1 (MQ=255) ccGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTg > 5:65685/1‑149 (MQ=255) cgTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTc < 1:173865/149‑1 (MQ=255) tATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGc < 1:355079/148‑1 (MQ=255) tGCATCAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCTGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCAt < 1:103678/148‑1 (MQ=255) caacaaACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATcac < 1:196652/149‑1 (MQ=255) aacaaACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCg > 4:277347/1‑135 (MQ=255) aacaaACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCg < 3:277347/135‑1 (MQ=255) aacaaACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATcacc > 3:10001/1‑149 (MQ=255) gaaagggCTGCATAGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCt > 3:149858/5‑147 (MQ=255) cTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAg > 7:37955/1‑149 (MQ=255) aaagggCTGCATAGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCGAGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCt > 1:111144/4‑147 (MQ=255) ggCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGa < 5:254020/149‑1 (MQ=255) tgctATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGAGCg > 8:12690/1‑149 (MQ=255) ctATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTAATGGCCGTGGTCTGCATCACAACCTCAGACGATCGtt > 6:175009/1‑149 (MQ=255) | GGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGCCGCATAGCG‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCC‑CGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCTTGCTACTGGCCGTGGTCTGCATCACCACCTCAGCCGAGCGTT > NC_002947/5555513‑5555795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |