Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,597,893 T→C 7.6% L465P (CTG→CCG)  PP_4923 → outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,597,8930TC 7.6% 269.0 / 8.8 92L465P (CTG→CCG) PP_4923outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base T (47/38);  new base C (5/2);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

ACTACAACAATAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTCAATATCA  >  NC_002947/5597746‑5598041
                                                                                                                                                    |                                                                                                                                                     
aCTACAACAATAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTg                                                                                                                                                      <  6:223713/149‑1 (MQ=255)
  tACAACAATAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTggc                                                                                                                                                    >  3:178084/1‑149 (MQ=255)
    caacaaTAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTggcgg                                                                                                                                                  >  2:86621/1‑149 (MQ=255)
    caacaaTAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTggcgg                                                                                                                                                  >  2:162728/1‑149 (MQ=255)
    caacaaTAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTggcg                                                                                                                                                   <  4:178084/148‑1 (MQ=255)
             ccGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCgg                                                                                                                                         <  2:229133/149‑1 (MQ=255)
               gTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCGG‑CCGGACCTGGCGGGAGCAGCGGcc                                                                                                                                       <  8:227771/149‑1 (MQ=255)
               gTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGcc                                                                                                                                       <  3:351508/149‑1 (MQ=255)
                  aCGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCaa                                                                                                                                     <  3:56773/148‑1 (MQ=255)
                      cTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACt                                                                                                                                >  3:292456/1‑149 (MQ=255)
                         cATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTc                                                                                                                              >  1:374218/1‑148 (MQ=255)
                         cATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTc                                                                                                                              <  5:216092/148‑1 (MQ=255)
                         aaTTATCGACAACCTGAGCCTGAAGCAGGGAGCCGTAACCCTGAGCCCGCTAGACCTTGAGGACGTGAAGCGCTACCTGAAACTTGACTACAACCATGACAATGACTTACTGCGG‑CATGACCTGGCGGAAGCAGCTGACAATAACTTc                                                                                                                              <  5:171238/147‑1 (MQ=255)
                          aTTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGTAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGa                                                                                                                            <  6:242048/149‑1 (MQ=255)
                          aTTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGa                                                                                                                            <  4:63744/149‑1 (MQ=255)
                           ttCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCAGGTCCGGCGgcagg                                                                                                                                              <  4:110755/130‑2 (MQ=255)
                           ttCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCAGGTCCGGCGgcagg                                                                                                                                              >  3:110755/1‑129 (MQ=255)
                               cGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAgcg                                                                                                                         >  3:367249/1‑147 (MQ=255)
                                gACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAgcgc                                                                                                                        <  2:256480/147‑1 (MQ=255)
                                   aaCCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGcc                                                                                                                   >  1:95170/1‑149 (MQ=255)
                                   aaCCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCCGACCTGGCGGCAGCCGCGGCCAAGAACTTCGAGCGCAGGcc                                                                                                                   >  5:319934/1‑149 (MQ=255)
                                     ccTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCtt                                                                                                                 >  7:296219/1‑149 (MQ=255)
                                     ccTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCtt                                                                                                                 <  3:243404/149‑1 (MQ=255)
                                      cTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCtt                                                                                                                 >  3:247744/1‑148 (MQ=255)
                                       tGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCtt                                                                                                                 >  7:303097/1‑147 (MQ=255)
                                       tGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCtt                                                                                                                 >  8:210922/1‑147 (MQ=255)
                                         aGCCTGAAGCATGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTga                                                                                                               <  5:293538/147‑1 (MQ=255)
                                         aGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCGCCCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTga                                                                                                               <  1:86621/148‑1 (MQ=255)
                                           ccTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTgaga                                                                                                             >  2:280815/1‑147 (MQ=255)
                                            cTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAg                                                                                                          >  1:9113/1‑149 (MQ=255)
                                               aaGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAgg                                                                                                         <  6:66910/147‑1 (MQ=255)
                                                     gcagCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTggtc                                                                                                 >  8:150744/1‑147 (MQ=255)
                                                                ccTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGc                                                                                      >  1:23801/1‑149 (MQ=255)
                                                                ccTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGc                                                                                      >  6:253407/1‑149 (MQ=255)
                                                                  tGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCtt                                                                                    >  2:184608/1‑149 (MQ=255)
                                                                       ccGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGCCCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCg                                                                               >  3:229095/1‑149 (MQ=255)
                                                                        cGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAgg                                                                                                         <  5:381637/122‑1 (MQ=255)
                                                                        cGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAgg                                                                                                         >  6:381637/1‑122 (MQ=255)
                                                                        cGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCAGc                                                                              >  5:308755/1‑149 (MQ=255)
                                                                         gCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAg                                                                                                                           >  3:288640/1‑103 (MQ=255)
                                                                         gCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAg                                                                                                                           <  4:288640/103‑1 (MQ=255)
                                                                         gCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAgggg                                                                                                       >  5:75417/1‑123 (MQ=255)
                                                                         gCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAgggg                                                                                                       <  6:75417/123‑1 (MQ=255)
                                                                             gACCTGCAGGACCTGACGAGCTACCTGAAACCTGCCTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCca                                                                         <  8:296219/149‑1 (MQ=255)
                                                                             gACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCca                                                                         >  5:339891/1‑149 (MQ=255)
                                                                              aCCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCca                                                                         >  1:231458/1‑148 (MQ=255)
                                                                              aCCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCAAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCca                                                                         >  1:336720/1‑148 (MQ=255)
                                                                                cTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCa‑cac                                                                     >  2:343747/1‑149 (MQ=255)
                                                                                   cAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGt                                                                  <  4:367249/149‑1 (MQ=255)
                                                                                    aGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTg                                                                 <  1:184608/149‑1 (MQ=255)
                                                                                     ggACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGc                                                                <  1:162728/149‑1 (MQ=255)
                                                                                         cTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAgg                                                            >  6:203669/1‑149 (MQ=255)
                                                                                         cTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAgg                                                            <  2:56481/149‑1 (MQ=255)
                                                                                          tGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGc                                                           >  1:331132/1‑149 (MQ=255)
                                                                                           gAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGcc                                                          >  3:83492/1‑149 (MQ=255)
                                                                                            aaGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGc                                                           <  1:343747/147‑1 (MQ=255)
                                                                                            aaGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCt                                                         >  3:330235/1‑149 (MQ=255)
                                                                                              gcgcTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGa                                                       <  4:292456/149‑1 (MQ=255)
                                                                                                  tACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAgg                                                    >  3:253332/1‑148 (MQ=255)
                                                                                                    ccTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGc                                                   <  2:23801/147‑1 (MQ=255)
                                                                                                    ccTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCat                                                 <  2:374218/149‑1 (MQ=255)
                                                                                                    ccTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCat                                                 >  3:58472/1‑149 (MQ=255)
                                                                                                     cTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCat                                                 >  2:367074/1‑148 (MQ=255)
                                                                                                          aCCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCa                                           >  3:292163/1‑149 (MQ=255)
                                                                                                           ccTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCAc                                          >  1:288487/1‑149 (MQ=255)
                                                                                                           ccTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCAAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCAc                                          <  2:336720/149‑1 (MQ=255)
                                                                                                            cTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCAcc                                         >  8:342527/1‑149 (MQ=255)
                                                                                                            cTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTACCAGCACGGTGCCAGGCCTGAAGGCATGATGCAc                                          <  1:33668/149‑1 (MQ=255)
                                                                                                              gACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCtg                                       >  8:158664/1‑149 (MQ=255)
                                                                                                                     aCCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGc                                >  5:251311/1‑149 (MQ=255)
                                                                                                                     aCCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGc                                >  7:201393/1‑149 (MQ=255)
                                                                                                                      cccGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCGTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCt                               >  8:39300/1‑149 (MQ=255)
                                                                                                                       ccGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTg                              >  7:267090/1‑149 (MQ=255)
                                                                                                                        cGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTgg                             >  2:364655/1‑149 (MQ=255)
                                                                                                                        cGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTgg                             <  7:210922/149‑1 (MQ=255)
                                                                                                                              aGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGCGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCt                       <  2:9113/149‑1 (MQ=255)
                                                                                                                               ggACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTg                      >  6:205532/1‑149 (MQ=255)
                                                                                                                               ggACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTg                      <  4:292163/149‑1 (MQ=255)
                                                                                                                                gACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTgg                     <  6:339891/149‑1 (MQ=255)
                                                                                                                                gACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTgg                     <  8:201393/149‑1 (MQ=255)
                                                                                                                                    tgcTGCCG‑CCAGGTCCGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTg                      <  1:109182/142‑1 (MQ=255)
                                                                                                                                    tgcTGCCG‑CCAGGTCCGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTg                      >  2:109182/3‑144 (MQ=255)
                                                                                                                                      cTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGACTGAAGGCATGATGCACCTGTGAGCGCTGGCTTGCTGGCGATAg               >  4:15564/1‑149 (MQ=255)
                                                                                                                                      cTGCCG‑CCAGGTCCGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAg               >  5:347862/1‑149 (MQ=255)
                                                                                                                                      cTGCCG‑CCAGGTCCGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAg               >  6:152283/1‑149 (MQ=255)
                                                                                                                                       tGCCG‑CCAGGTCCGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAgg              >  8:303081/1‑149 (MQ=255)
                                                                                                                                         ccg‑ccgGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAgg              <  4:58472/147‑1 (MQ=255)
                                                                                                                                         ccg‑ccgGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGcc            >  5:344684/1‑149 (MQ=255)
                                                                                                                                         ccg‑ccgGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGc             <  7:158664/148‑1 (MQ=255)
                                                                                                                                              cgGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTc         >  2:327073/1‑148 (MQ=255)
                                                                                                                                              cgGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTCa        <  5:203669/149‑1 (MQ=255)
                                                                                                                                               ggACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTCaa       <  8:303097/149‑1 (MQ=255)
                                                                                                                                                    tGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTCAATATCa  >  5:364914/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACTACAACAATAGCCGTTACGACTACATTCTCGACAACCTGAGCCTGAAGCAGGCAGCCGGCACCCTGAGCCCGCAAGACCTGCAGGACCTGAAGCGCTACCTGAAACCTGACTACAACCCGGACAAGGACTTCCTGCCG‑CCGGACCTGGCGGCAGCAGCGGCCAAGAACTTCGAGCGCAGGCCTTGAGATAGGGGCTGGACTCTTCGCGGGCTTGCCCGCTCCCA‑CACGGTGCCAGGCCTGAAGGCATGATGCACCTGTGAGAGCTGGCTTGCTGGCGATAGGCCTTCAATATCA  >  NC_002947/5597746‑5598041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑