Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 418,295 C→G 6.9% L26L (CTC→CTG)  PP_0345 → heptose kinase WapP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947418,2950CG 6.9% 247.0 / 6.3 79L26L (CTC→CTG) PP_0345heptose kinase WapP
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base G (2/3);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

GCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGG  >  NC_002947/418149‑418439
                                                                                                                                                  |                                                                                                                                                
gctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCa                                                                                                                                                <  3:10698/149‑1 (MQ=255)
    gCCTGGGTGTAGCGCAAGGCCCAGTAACTCTAGGATGGGTAGCATCGCTATGGGGATGCAGTCTGATGGGTTGTTGGACTCTGGCTGCGGGGTACGAACACCTGGCGGCGGAGTTCGGCAGCCTCGACGCGGTGTTGGCCCTTGAggg                                                                                                                                             <  8:267029/148‑1 (MQ=255)
    gCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGc                                                                                                                                            <  2:144595/149‑1 (MQ=255)
      ctggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCg                                                                                                                                           >  1:301161/1‑148 (MQ=255)
        ggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTGTGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                        >  1:264534/1‑149 (MQ=255)
          ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcac                                                                                                                                      <  1:215869/149‑3 (MQ=255)
          ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                      <  2:335697/149‑1 (MQ=255)
           tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                     >  6:135133/1‑149 (MQ=255)
              aGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGTCTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                  <  4:127117/149‑1 (MQ=255)
                cgcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacc                                                                                                                                >  1:260840/1‑149 (MQ=255)
                 gcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                              >  2:341538/1‑134 (MQ=255)
                 gcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                              <  1:341538/134‑1 (MQ=255)
                   aaGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgc                                                                                                                             <  2:22171/149‑1 (MQ=255)
                        ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc                                                                                                                        >  5:51259/1‑149 (MQ=255)
                        ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc                                                                                                                        >  6:253318/1‑149 (MQ=255)
                             aaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                   <  7:336119/149‑1 (MQ=255)
                             aaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                   <  3:118152/149‑1 (MQ=255)
                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAca                                                                                                                                       >  5:331791/1‑125 (MQ=255)
                                tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAca                                                                                                                                       <  6:331791/126‑2 (MQ=255)
                                 ctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCCGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTg                                                                                                                >  1:118689/1‑148 (MQ=255)
                                   aCGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                  >  8:23065/1‑128 (MQ=255)
                                   aCGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTga                                                                                                                                  <  7:23065/128‑1 (MQ=255)
                                    cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGt                                                                                                            <  1:88810/149‑1 (MQ=255)
                                     gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg                                                                                                           <  6:51259/149‑1 (MQ=255)
                                       tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGt                                                                                                         <  7:243178/149‑1 (MQ=255)
                                         gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAg                                                                                                             >  8:73947/1‑143 (MQ=255)
                                         gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAg                                                                                                             <  7:73947/143‑1 (MQ=255)
                                         gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTAc                                                                                                       <  5:201272/149‑1 (MQ=255)
                                          caagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACg                                                                                                      >  8:28051/1‑149 (MQ=255)
                                               aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                              >  2:21967/1‑104 (MQ=255)
                                               aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                              <  1:21967/104‑1 (MQ=255)
                                               aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                        >  7:120377/1‑110 (MQ=255)
                                               aaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAc                                                                                                                                        <  8:120377/110‑1 (MQ=255)
                                                aCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgaccc                                                                                                                               <  8:118878/118‑1 (MQ=255)
                                                aCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgaccc                                                                                                                               >  7:118878/1‑118 (MQ=255)
                                                  gCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc                                                                                              <  5:348938/149‑1 (MQ=255)
                                                    tATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGt                                                                                            >  7:93222/1‑149 (MQ=255)
                                                    tATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGt                                                                                            >  3:122617/1‑149 (MQ=255)
                                                        gggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                        <  4:183564/149‑1 (MQ=255)
                                                        gggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                        <  2:118689/149‑1 (MQ=255)
                                                            tGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                                    <  6:178347/149‑1 (MQ=255)
                                                            tGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                                    <  1:287127/149‑1 (MQ=255)
                                                              cACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCa                                                                                  <  2:260840/149‑1 (MQ=255)
                                                                 tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACggg                                                                                      >  4:323169/1‑142 (MQ=255)
                                                                 tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACggg                                                                                      <  3:323169/142‑1 (MQ=255)
                                                                 tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                >  2:119849/1‑148 (MQ=255)
                                                                     aTGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAActacta                                                                           <  7:28051/149‑1 (MQ=255)
                                                                     aTGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAActacta                                                                           <  5:253318/149‑1 (MQ=255)
                                                                          gAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAact                                                                               <  4:140686/140‑1 (MQ=255)
                                                                          gAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAact                                                                               >  3:140686/1‑140 (MQ=255)
                                                                                gACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcg                                                                                                                            >  2:157452/1‑89 (MQ=255)
                                                                                gACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcg                                                                                                                            <  1:157452/89‑1 (MQ=255)
                                                                                   acTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGTAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTAc                                                                                                       <  5:273179/107‑1 (MQ=255)
                                                                                   acTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGTAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTAc                                                                                                       >  6:273179/1‑107 (MQ=255)
                                                                                   acTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTaca                                                             >  2:196993/1‑149 (MQ=255)
                                                                                             gggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCgg                                                   >  6:283218/1‑149 (MQ=255)
                                                                                                 aCGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAg                                               >  8:178637/1‑149 (MQ=255)
                                                                                                   gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAACCGCTACACCGGGGCCGGCAAGca                                             >  8:156450/1‑149 (MQ=255)
                                                                                                      cacCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACAt                                          <  4:122617/149‑1 (MQ=255)
                                                                                                                 ggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCAACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAcc                               >  5:346371/1‑149 (MQ=255)
                                                                                                                   aCTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGCCCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCATCTACTACGTCACGCGCTACACCGGGGCGGGCAAGCACATGCGCCGGTACCTg                             >  8:272722/1‑149 (MQ=255)
                                                                                                                     ttCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTggg                           >  8:51898/1‑149 (MQ=255)
                                                                                                                         gCAGCCTCGACGCGGTGGTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcgg                       <  8:68262/149‑1 (MQ=255)
                                                                                                                              cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                  <  8:93222/149‑1 (MQ=255)
                                                                                                                                     cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAg           <  7:51898/149‑1 (MQ=255)
                                                                                                                                       gtgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGc         <  4:270763/149‑1 (MQ=255)
                                                                                                                                        tgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc        >  3:169652/1‑149 (MQ=255)
                                                                                                                                         gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                        <  5:103156/100‑1 (MQ=255)
                                                                                                                                         gtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                        >  6:103156/1‑100 (MQ=255)
                                                                                                                                          ttCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCg       >  6:202514/1‑148 (MQ=255)
                                                                                                                                           tCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCGAGCACATGCGCCGGTACCTGGggcg                        <  5:22589/130‑1 (MQ=255)
                                                                                                                                           tCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCGAGCACATGCGCCGGTACCTGGggcg                        >  6:22589/1‑130 (MQ=255)
                                                                                                                                            cGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt    >  5:185030/1‑149 (MQ=255)
                                                                                                                                            cGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt    >  7:253122/1‑149 (MQ=255)
                                                                                                                                             gCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGaa     >  1:321493/1‑147 (MQ=255)
                                                                                                                                              cccTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATgg  >  1:329167/1‑149 (MQ=255)
                                                                                                                                              cccTCCAGGGCGAACGGCTGAACCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCTGGCATGGACATGCGCCGGTACCTGGGGCGGCCACGCATCAACGACGAATgg  >  6:305049/1‑149 (MQ=255)
                                                                                                                                               ccTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATgg  >  5:276855/1‑148 (MQ=255)
                                                                                                                                                 tCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCgg                                                   <  6:266093/97‑1 (MQ=255)
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GCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGG  >  NC_002947/418149‑418439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑