Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,608,994 G→C 5.2% R202G (CGC→GGC)  waaA ← 3‑deoxy‑D‑manno‑octulosonic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,608,9940GC 5.2% 313.0 / 5.4 96R202G (CGC→GGC) waaA3‑deoxy‑D‑manno‑octulosonic acid transferase
Reads supporting (aligned to +/- strand):  ref base G (45/46);  new base C (3/2);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

GCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTG  >  NC_002947/5608852‑5609139
                                                                                                                                               |                                                                                                                                                     
gCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGACTCGAGCCGGTGACTTGCACGCGCTCAg                                                                                                                                                  <  1:206860/149‑1 (MQ=255)
  aTCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGc                                                                                                                                               >  4:52462/1‑149 (MQ=255)
  aTCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGc                                                                                                                                               <  2:166353/149‑1 (MQ=255)
    cTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTAACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGt                                                                                                                                             >  2:159007/1‑149 (MQ=255)
       cACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCa                                                                                                                                          >  2:194857/1‑149 (MQ=255)
        aCCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                         <  1:164360/149‑2 (MQ=255)
         ccGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGc                                                                                                                                               <  5:19389/142‑1 (MQ=255)
         ccGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGc                                                                                                                                               >  6:19389/1‑142 (MQ=255)
         ccGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCAcc                                                                                                                                        >  6:235375/1‑149 (MQ=255)
          cgtcgtGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCAccc                                                                                                                                       <  4:196892/149‑1 (MQ=255)
            tcgtGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAg                                                                                                                                     <  1:194857/149‑1 (MQ=255)
              gtGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGt                                                                                                                                   >  3:362676/1‑149 (MQ=255)
                  gTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCTCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACg                                                                                                                               >  5:116182/1‑149 (MQ=255)
                   tGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGGCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACgg                                                                                                                              <  8:65152/149‑1 (MQ=255)
                    gCTGGCGGCAATCCACACACGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGa                                                                                                                             <  5:38220/149‑1 (MQ=255)
                     cTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACgg                                                                                                                              >  7:197146/1‑147 (MQ=255)
                     cTGGCGGCAATCCACACAGGCCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATCCTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACTCGCTCAGGGCGTGCACCCAGGTCACGGa                                                                                                                             <  5:43624/148‑1 (MQ=255)
                      tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTc                                                                                                                                  >  3:361780/1‑142 (MQ=255)
                      tGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTc                                                                                                                                  <  4:361780/142‑1 (MQ=255)
                        gcggcAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAgc                                                                                                                          <  3:362520/148‑1 (MQ=255)
                            cAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTgg                                                                                                                     <  2:196635/149‑1 (MQ=255)
                                 cacacaGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                         >  3:84808/1‑123 (MQ=255)
                                 cacacaGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCCCTGAGCGCGTGCaa                                                                                                                                         <  4:84808/124‑2 (MQ=255)
                                 cacacaGGGCGCTGGCCGCCCCCAAACTCTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTTTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTc                                                                                                                <  6:116182/149‑1 (MQ=255)
                                  acacaGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCg                                                                                                               >  7:179982/1‑149 (MQ=255)
                                        ggCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                         <  6:258054/149‑1 (MQ=255)
                                          cgcTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGt                                                                                                       <  8:179982/149‑1 (MQ=255)
                                           gcTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCCGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTc                                                                                                      >  6:173408/1‑149 (MQ=255)
                                             tGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑g                                                                                                   >  1:40442/1‑149 (MQ=255)
                                                 cgccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACg                                                                                               >  5:302927/1‑149 (MQ=255)
                                                  gccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              <  5:235375/149‑1 (MQ=255)
                                                  gccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              <  8:197146/149‑1 (MQ=255)
                                                  gccCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              >  3:304931/1‑149 (MQ=255)
                                                  cgcccccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              <  4:253383/147‑1 (MQ=255)
                                                       ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                         <  2:31399/134‑1 (MQ=255)
                                                       ccAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑Tc                                                                                      <  3:52462/149‑1 (MQ=255)
                                                       ccAACTGTTCACGCAATGCCCTCGCGCGTGCCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCg                                                                                                         >  1:31399/1‑134 (MQ=255)
                                                          aCTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAgg                                                                                   <  2:40442/149‑1 (MQ=255)
                                                          aCTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAgg                                                                                   >  2:273654/1‑149 (MQ=255)
                                                               tCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAgg                                                                                                                                                 >  1:179054/1‑86 (MQ=255)
                                                               tCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAgg                                                                                                                                                 <  2:179054/86‑1 (MQ=255)
                                                                 aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCAtct                                                                            >  4:217587/1‑149 (MQ=255)
                                                                 aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCAtct                                                                            >  2:291113/1‑149 (MQ=255)
                                                                 aCGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCAtc                                                                             <  5:316450/148‑1 (MQ=255)
                                                                   gCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCg                                                                          >  5:264545/1‑149 (MQ=255)
                                                                   gCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCg                                                                          >  3:115594/1‑149 (MQ=255)
                                                                    cAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCgg                                                                         <  6:302927/149‑1 (MQ=255)
                                                                     aaTGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGc                                                                                  <  8:329309/139‑1 (MQ=255)
                                                                     aaTGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGc                                                                                  >  7:329309/1‑139 (MQ=255)
                                                                     aaTGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGc                                                                        <  1:75691/149‑1 (MQ=255)
                                                                          ccTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCg                                                                                                               <  2:180506/109‑1 (MQ=255)
                                                                          ccTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCg                                                                                                               >  1:180506/1‑109 (MQ=255)
                                                                          ccTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGc                                                                    >  4:253931/1‑148 (MQ=255)
                                                                           cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              <  6:261222/124‑1 (MQ=255)
                                                                           cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCa                                                                      >  6:170467/1‑145 (MQ=255)
                                                                           cTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCa                                                                      <  5:170467/145‑1 (MQ=255)
                                                                            tCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATc                                                                 >  1:24573/1‑149 (MQ=255)
                                                                            tCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATc                                                                 >  1:30253/1‑149 (MQ=255)
                                                                              gcgcgTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCgg                                                               >  2:289084/1‑149 (MQ=255)
                                                                                 cgTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCg                                                            <  1:273654/149‑1 (MQ=255)
                                                                                  gTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCgg                                                           <  4:304931/149‑1 (MQ=255)
                                                                                  gTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCgg                                                           <  2:185069/149‑1 (MQ=255)
                                                                                  gTGGCAGCAGCTGTTCGGCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGCGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCCGCCgg                                                           <  6:180039/149‑1 (MQ=255)
                                                                                       agcagcTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAg                                                      <  6:316217/149‑1 (MQ=255)
                                                                                        gcagcTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGc                                                     >  7:169254/1‑149 (MQ=255)
                                                                                            cTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCACCTCGGCCAGCATCGGCCGGGTCAGCCTgg                                                 <  8:148084/149‑1 (MQ=255)
                                                                                               ttCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                <  2:30253/147‑1 (MQ=255)
                                                                                               ttCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGc                                                <  2:24573/147‑1 (MQ=255)
                                                                                                  gtcgATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgc                                           >  4:116205/1‑149 (MQ=255)
                                                                                                  gtcgATGGACAGGTCGAACTTGA‑TAGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACACAGGTCACGGAAGCGCTCGGCCTCGGATTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGCCCAGCATCGGACGGGTCAGCCTGCCAAAgc                                           >  3:219897/1‑149 (MQ=255)
                                                                                                  ggcgATGGGCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgc                                           <  8:297519/147‑1 (MQ=255)
                                                                                                    cgATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAgcgc                                         <  6:264545/149‑1 (MQ=255)
                                                                                                      aTGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCTAGCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGcc                                        <  1:289084/149‑1 (MQ=255)
                                                                                                       tGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGGCTCg                                                                                                               >  4:182350/1‑80 (MQ=255)
                                                                                                       tGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCg                                                                                                               <  3:182350/80‑1 (MQ=255)
                                                                                                        ggTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCa                                      >  8:213873/1‑148 (MQ=255)
                                                                                                         gTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATa                                    <  3:253931/149‑1 (MQ=255)
                                                                                                          tCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGCTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAg                                   >  1:119754/1‑149 (MQ=255)
                                                                                                           cAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              <  5:199094/92‑1 (MQ=255)
                                                                                                           cAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACgg                                                                                              >  6:199094/1‑92 (MQ=255)
                                                                                                            aGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGc                                  >  1:13459/1‑148 (MQ=255)
                                                                                                              gTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCATGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                               >  5:295248/1‑149 (MQ=255)
                                                                                                              gTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                               >  2:97847/1‑149 (MQ=255)
                                                                                                              gTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                               <  3:217587/149‑1 (MQ=255)
                                                                                                              gTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCAc                               >  3:211053/1‑149 (MQ=255)
                                                                                                                cGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACgg                             >  5:10073/1‑149 (MQ=255)
                                                                                                                     ttGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGcc                          >  2:183367/1‑147 (MQ=255)
                                                                                                                            gAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCAGCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCt                   <  3:83094/148‑1 (MQ=255)
                                                                                                                              gCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGTTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCt                   >  8:289870/1‑146 (MQ=255)
                                                                                                                                   ctgggtGCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAg           >  8:224134/6‑149 (MQ=255)
                                                                                                                                    gACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgc          >  4:82020/1‑149 (MQ=255)
                                                                                                                                       ttGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcgcg       >  8:139123/1‑149 (MQ=255)
                                                                                                                                       ttGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcgcg       >  1:236866/1‑149 (MQ=255)
                                                                                                                                         gCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcgcg       <  7:139123/147‑1 (MQ=255)
                                                                                                                                         gCACGCCCTGAGCGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGAAAGGCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAgcgc        >  1:111202/1‑149 (MQ=255)
                                                                                                                                           aCGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCAtt   <  3:82020/149‑1 (MQ=255)
                                                                                                                                            cgcgCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg  <  4:362676/149‑1 (MQ=255)
                                                                                                                                            cgcgCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTg  <  4:115594/149‑1 (MQ=255)
                                                                                                                                             gcgcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACggg                            <  7:314367/122‑1 (MQ=255)
                                                                                                                                             gcgcTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACggg                            >  8:314367/1‑122 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCATCTTCACCGTCGTGGGTGCTGGCGGCAATCCACACAGGGCGCTGGCCGCCCCCCAACTGTTCACGCAATGCCCTCGCGCGTGGCAGCAGCTGTTCGTCGATGGTCAGGTCGAACTTGA‑TCGAGCCGGTGACTTGCACGCGCTCAGGGCGTGCACCCAGGTCACGGAAGCGCTGGGCCTCGGTTTCGGTCT‑GCACGGCGA‑‑‑TCAGGCTCATCTCGGCCAGCATCGGCCGGGTCAGCCTGGCAAAGCGCCCATAGCCACGGGCCGAGCGCTCAGACAAGCGCGCATTG  >  NC_002947/5608852‑5609139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑