Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,612,017	C→G	10.0%	R337R (CGC→CGG) ‡	PP_4931 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,612,017	0	C	G	10.0%	168.6 / 10.2	60	R337R (CGC→CGG) ‡	PP_4931	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/28);  new base G (2/4);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

TTGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGG  >  NC_002947/5611869‑5612164                                                                                                                                                     |                                                                                                                                                    ttGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAgcgc                                                                                                                                                     <  5:271080/149‑1 (MQ=255) ttGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAgcgc                                                                                                                                                     <  5:276239/149‑1 (MQ=255)         gcgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGGCGCTGCtggt                                                                                                                                             <  3:316265/149‑1 (MQ=255)         gcgcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCtggt                                                                                                                                             <  8:240988/149‑1 (MQ=255)           gcagAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCCACCAGCAGGCGCTGCtggtcg                                                                                                                                           <  4:224176/149‑3 (MQ=255)              gAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCt                                                                                                                                        >  2:26002/1‑149 (MQ=255)              gAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCt                                                                                                                                        <  3:167718/149‑1 (MQ=255)               aaGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTAGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTg                                                                                                                                       >  1:87589/1‑149 (MQ=255)                acgAAATCGACCACCCGCTGCCATGGGTCGATCAGAGCCTGGTTCGCTGGGCCACACTGGGCGTCGACCGCGCGGACCCTGGACAATGCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCCACCAGCAGGCGCTGCTGGTCGGcaag                                                                                                                                      <  2:41427/147‑4 (MQ=255)                aGGAAATCGACACCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTgg                                                                                                                                      <  4:64431/149‑1 (MQ=255)                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCtggt                                                                                                                                             <  5:135637/141‑1 (MQ=255)                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCtggt                                                                                                                                             >  6:135637/1‑141 (MQ=255)                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGc                                                                                                                                     <  6:340334/149‑1 (MQ=255)                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGc                                                                                                                                     <  6:272944/149‑1 (MQ=255)                 ggAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGACACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGCCTGGc                                                                                                                                     >  7:311925/1‑149 (MQ=255)                  gAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCATCGCCTGCTGGTGGGCTGGcc                                                                                                                                    >  5:305472/1‑149 (MQ=255)                   aaaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCg                                                                                                                                   <  5:355372/149‑1 (MQ=255)                    aaTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGa                                                                                                                                  >  2:266847/1‑149 (MQ=255)                     aTCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGAc                                                                                                                                 <  6:217834/149‑1 (MQ=255)                         aCAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCaaa                                                                                                                             >  1:93216/1‑149 (MQ=255)                         aCAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGAGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCaaa                                                                                                                             >  1:98264/1‑149 (MQ=255)                           aaCCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAact                                                                                                                           <  4:104817/149‑1 (MQ=255)                             ccTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactgg                                                                                                                         <  5:278717/149‑1 (MQ=255)                             ccTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactgg                                                                                                                         >  1:376264/1‑149 (MQ=255)                                 ctgcCTTGGGTCGATCAGAGCGTGGTGCGCGGGGCCACACTGCGCGTCGACCGCGCTGAGCCGGCACATTCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggcac                                                                                                                      <  2:364101/148‑1 (MQ=255)                                 ctgcCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggca                                                                                                                       <  1:26002/147‑1 (MQ=255)                                 ctgcCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGAACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCAGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggcac                                                                                                                      >  1:364101/1‑148 (MQ=255)                                 atgcCCTGGGGCGTTCAGAGCCTGGTGCGCTGGGCCACTCTGCGCGTCGACCGCGCTGAGCCTGCACAATCCTGCCTGGCACGCCCGGACAACGCGTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggcact                                                                                                                     <  8:311925/148‑1 (MQ=255)                                    ccATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAactggcactgg                                                                                                                   >  7:55417/1‑148 (MQ=255)                                         ggTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCgg                                                                                                             >  8:351429/1‑149 (MQ=255)                                         ggTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCg                                                                                                              <  1:266847/148‑1 (MQ=255)                                           tCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGAc                                                                                                           >  4:183164/1‑149 (MQ=255)                                             gATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACtt                                                                                                         <  2:376264/149‑1 (MQ=255)                                             gATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACt                                                                                                          <  3:183164/148‑1 (MQ=255)                                                     ccTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCctgctg                                                                                                                                               <  8:257647/103‑1 (MQ=255)                                                     ccTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCctgctg                                                                                                                                               >  7:257647/1‑103 (MQ=255)                                                         gTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCggg                                                                                              >  1:352104/1‑148 (MQ=255)                                                                       acTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTgg                                                                               <  2:36877/149‑1 (MQ=255)                                                                                cGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACg                                                                      <  2:348862/149‑1 (MQ=255)                                                                                 gACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACgg                                                                     <  7:351429/149‑1 (MQ=255)                                                                                    cgcgCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCAt                                                                  <  2:352104/149‑1 (MQ=255)                                                                                           aGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTc                                                             <  2:93216/147‑1 (MQ=255)                                                                                           aGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTc                                                             <  2:98264/147‑1 (MQ=255)                                                                                               tGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAg                                                       >  2:25406/1‑149 (MQ=255)                                                                                                gCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGGCGCTGCtggtcg                                                                                                                                           <  5:160050/64‑3 (MQ=37)                                                                                                gCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGGCGCTGCtggtcg                                                                                                                                           >  6:160050/1‑62 (MQ=37)                                                                                                 cacaATCCGGCCTGGCACGCCCGGAGAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGTCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAACCAGCCACCTGGAGCGCGACGGCATTCGTCCAGAAg                                                       <  6:305472/147‑1 (MQ=255)                                                                                                    aaTCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGc                                                      <  7:29444/145‑1 (MQ=255)                                                                                                    aaTCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGc                                                      >  8:29444/1‑145 (MQ=255)                                                                                                    aaTCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACAGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAg                                                  >  8:254327/1‑149 (MQ=255)                                                                                                          gCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGAc                                            >  2:40743/1‑149 (MQ=255)                                                                                                               gCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTga                                        <  1:40743/148‑1 (MQ=255)                                                                                                                   gCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTgaccgac                                   >  2:12717/1‑149 (MQ=255)                                                                                                                              gCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGccc                        >  7:70389/1‑149 (MQ=255)                                                                                                                               ccttccttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCt                       >  1:90388/1‑149 (MQ=255)                                                                                                                                tcggccagCCCACCAGCAGGCGCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTc                      >  3:323360/8‑149 (MQ=255)                                                                                                                                    cttGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCt                  <  8:55417/149‑1 (MQ=255)                                                                                                                                       gCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTggg               >  6:134599/1‑149 (MQ=255)                                                                                                                                            ccAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGCCCTGACCGACCTGCCACGCCCTCCGCTGGGCGTAc          >  2:248078/1‑149 (MQ=255)                                                                                                                                               gcagcGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGc              <  3:260411/142‑1 (MQ=255)                                                                                                                                               gcagcGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGc              >  4:260411/1‑142 (MQ=255)                                                                                                                                               gcagcGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCgg       >  3:247247/1‑149 (MQ=255)                                                                                                                                                    gcCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTgg  >  6:256278/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTGCCGCAGCGCAGAAGGAAATCGACAACCTGCTGCCATGGGTCGATCAGAGCCTGGTGCGCTGGGCCACACTGCGCGTCGACCGCGCTGAGCCTGCACAATCCGGCCTGGCACGCCCGGACAACGCCTTCCTTGCCGACCAGCAGCGCCTGCTGGTGGGCTGGCCGACCAAACTGGCACTGGCACCGGACTTCAGCGACCGGGTAATCAGCCACCTGGAGCGCGACGGCATTCGTCCACAAGCACAGGCCGACCTGACCGACCTGCCACGCCCTCCGCTGGGCGTACCGGCCTGG  >  NC_002947/5611869‑5612164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑