Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	424,632	G→A	7.0%	N226N (AAC→AAT)	PP_0349 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	424,632	0	G	A	7.0%	207.6 / 4.2	71	N226N (AAC→AAT)	PP_0349	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base A (2/3);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

TGGAGAAACGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTACGGTG  >  NC_002947/424484‑424779                                                                                                                                                        |                                                                                                                                                      tGGAGAAACGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCg                                                                                                                                                       >  2:49299/1‑149 (MQ=255) tGGAGAAACGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCg                                                                                                                                                       >  6:99204/1‑149 (MQ=255)   gagaAACGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGtt                                                                                                                                                     >  6:318402/1‑149 (MQ=255)      aaaCGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTgcg                                                                                                                                                  >  5:221746/1‑149 (MQ=255)         cgcgTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTgcgc                                                                                                                                                 >  3:361210/1‑147 (MQ=255)              gCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCg                                                                                                                                          >  7:37590/1‑149 (MQ=255)               ccTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCg                                                                                                                                          <  4:89242/148‑1 (MQ=255)                cTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GAGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCACCCATCGCAGCCAGGCTGTCGTTGAGCCAGGCCGc                                                                                                                                         >  4:331259/1‑148 (MQ=255)                 tGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCaa                                                                                                                                       >  3:56309/1‑149 (MQ=255)                 tGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCaa                                                                                                                                       >  3:366217/1‑149 (MQ=255)                   gAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTACGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATg                                                                                                                                     <  1:263939/149‑1 (MQ=255)                    aaaCGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGa                                                                                                                                    <  8:37590/149‑1 (MQ=255)                      aCGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCATTGCGGCCTGGCGCAACGAca                                                                                                                                  <  7:339060/149‑2 (MQ=255)                          gATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACGATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGt                                                                                                                              <  5:318402/149‑1 (MQ=255)                          gATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGt                                                                                                                              <  7:153749/149‑1 (MQ=255)                             gAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCAGGCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGa                                                                                                                                    >  1:265095/1‑141 (MQ=255)                             gAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGa                                                                                                                                    <  2:265095/140‑1 (MQ=255)                                gTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGCAGGCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGa                                                                                                                          >  1:107253/1‑149 (MQ=255)                                 tCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAg                                                                                                                       >  8:87086/1‑149 (MQ=255)                                 tCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAg                                                                                                                       <  6:221746/149‑1 (MQ=255)                                 tCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAg                                                                                                                       >  6:334561/1‑149 (MQ=255)                                 tCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAg                                                                                                                       <  1:49299/149‑1 (MQ=255)                                   cGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAgg                                                                                                                      <  3:294687/148‑1 (MQ=255)                                    gggCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGc                                                                                                                    >  3:292862/1‑149 (MQ=255)                                    gggCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCATTGCGGCCTGGCGCAACGAca                                                                                                                                  >  2:11270/1‑134 (MQ=255)                                    gggCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGATGTCATTGCGGCCTGGCGCAACGAca                                                                                                                                  <  1:11270/135‑2 (MQ=255)                                     ggCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCGGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCa                                                                                                                   <  3:331259/149‑1 (MQ=255)                                           tGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGAt                                                                                                              >  7:309317/1‑148 (MQ=255)                                              ccGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAcc                                                                                                          >  6:315274/1‑149 (MQ=255)                                              ccGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAcc                                                                                                          >  2:107712/1‑149 (MQ=255)                                               cGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAccc                                                                                                         <  6:325563/149‑1 (MQ=255)                                                ggCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAcccc                                                                                                        <  4:292862/149‑1 (MQ=255)                                                   gcgGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGGCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGt                                                                                                     <  5:99204/149‑1 (MQ=255)                                                       ccTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACg                                                                                                 >  4:61820/1‑149 (MQ=255)                                                        cTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGc                                                                                                <  3:82666/149‑1 (MQ=255)                                                         tGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAcc                                                                                                          <  6:173082/138‑1 (MQ=255)                                                         tGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTAcc                                                                                                          >  5:173082/1‑138 (MQ=255)                                                         tGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCt                                                                                               >  5:287351/1‑149 (MQ=255)                                                         tGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCATTGCGGCCTGGCGCAACGAca                                                                                                                                  >  4:319847/1‑113 (MQ=255)                                                         tGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCATTGCGGCCTGGCGCAACGAca                                                                                                                                  <  3:319847/114‑2 (MQ=255)                                                           ttCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCt                                                                                               <  5:315274/147‑1 (MQ=255)                                                              ggCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCg                                                                                          >  6:330968/1‑149 (MQ=255)                                                                cAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGt                                                                                         >  7:29077/1‑148 (MQ=255)                                                                    gTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCg                                                                                                                            <  2:129606/109‑1 (MQ=255)                                                                     tCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCg                                                                                          >  2:27064/1‑142 (MQ=255)                                                                     tCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCg                                                                                          <  1:27064/142‑1 (MQ=255)                                                                      ccA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTc                                                                                                    <  4:10468/131‑1 (MQ=255)                                                                      ccA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTc                                                                                                    >  3:10468/1‑131 (MQ=255)                                                                       ca‑gcagGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGcc                                                                                  >  1:1962/1‑148 (MQ=255)                                                                            agGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCccaggc                                                                             <  2:107253/149‑1 (MQ=255)                                                                                       tGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑a                                                                >  3:348930/1‑149 (MQ=255)                                                                                            ccgccgcTCGTCGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGc                                                             >  3:34830/5‑149 (MQ=255)                                                                                                 gcTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTTCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑CCGCCGAt                                                         >  6:197497/1‑148 (MQ=255)                                                                                                 gcTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGAt                                                         >  5:61348/1‑148 (MQ=255)                                                                                                  cTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCa                                                       <  5:330968/149‑1 (MQ=255)                                                                                                   tCGACGAGCGGCGGGGCGTCGAGGTAGGGGGCAACCATCGCAGACAGGCTGTCGTTGCTCCAGGCCTCTATGACCAGGTCGGAGAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCGGATCAg                                                      <  6:366474/149‑1 (MQ=255)                                                                                                     gacgaGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAgg                                                     <  1:247922/148‑1 (MQ=255)                                                                                                        gaGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGGACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTtgt                                                 >  7:243188/1‑149 (MQ=255)                                                                                                          gcggcgGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGc                                               <  3:186110/149‑1 (MQ=255)                                                                                                          gcggcgGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGc                                               <  3:61820/149‑1 (MQ=255)                                                                                                           cggcggGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCa                                              <  4:366217/149‑1 (MQ=255)                                                                                                             gcggGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAgg                                            <  4:56309/149‑1 (MQ=255)                                                                                                              cggGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCCGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGt                                           >  3:58056/1‑149 (MQ=255)                                                                                                                  gCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAg                                       >  5:153130/1‑149 (MQ=255)                                                                                                                        cgGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAgcg                                 >  3:154156/1‑149 (MQ=255)                                                                                                                                     ccATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGAt                    >  8:6936/1‑149 (MQ=255)                                                                                                                                          gCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCtt               <  6:61348/149‑1 (MQ=255)                                                                                                                                            aGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTtgt             <  2:1962/149‑1 (MQ=255)                                                                                                                                             gCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTtgtg            >  5:58787/1‑149 (MQ=255)                                                                                                                                             gCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCAGCGGGATTTCTtgtg            <  4:350224/149‑1 (MQ=255)                                                                                                                                                  gCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTa       >  2:22552/1‑149 (MQ=255)                                                                                                                                                     gTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGGACTGATTACCCCGGTCACGCTGACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTACgg    >  1:98940/1‑149 (MQ=255)                                                                                                                                                      tCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCAAGCTCACCGTCAATGCCCAGGCCAGGGTGACCTTCCGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTACg     <  1:107712/149‑1 (MQ=255)                                                                                                                                                        gTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTACGGTg  <  4:154156/148‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                      TGGAGAAACGCGTGCCTGGAAACGCGATGAAGTCCGGGCGCATGCCCGGCGCGGCCTGTTCGGCAATGTCCA‑GCAGGCGAGTGGCTGGAGC‑‑GCGCTCGACGAGCGGCGGGGCGTCGCGGTAGGGGGCAACCATCGCAGCCAGGCTGTCGTTGCGCCAGGCCGCAATGACCAGGTCGGACAGGGCACTGATTACCCCGGTCACGCTCACCGTCAATGCCCAGGCCAGGGTGACC‑‑ACGCCGATCAGGTTGTGCAGGTCGAGCCAGCGCAGGCGGCGGGATTTCTTGTGGCGTACGGTG  >  NC_002947/424484‑424779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑