Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,697,318 T→G 7.4% intergenic (+28/‑49) hslV → / → hslU ATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,697,3180TG 7.4% 255.3 / 6.8 88intergenic (+28/‑49)hslV/hslUATP‑dependent HslVU protease peptidase subunit/protease HslVU ATPase subunit
Reads supporting (aligned to +/- strand):  ref base T (39/42);  new base G (3/4);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAACCG  >  NC_002947/5697174‑5697464
                                                                                                                                                |                                                                                                                                                  
aaCAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCAGTGaa                                                                                                                                                 <  2:8398/148‑1 (MQ=255)
 aCAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAgg                                                                                                                                               <  6:61402/149‑1 (MQ=255)
  cAAGACCTACCTCTCGGCCAGCGAAATCGCCGAGACCTCCCTGAACAACGCCGGCGACATCATCCTCATCACCAACCACAACCTGACCATCGAGGAGCACTACCAGGCCGACTGAATCAGCTGTTCTGGCGCCCATTCCAGGTGAAggg                                                                                                                                              >  2:25168/1‑149 (MQ=255)
  cAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAggg                                                                                                                                              >  6:375986/1‑149 (MQ=255)
  cAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCCGCGACATCTGCGTCTTCACCACCCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCACCTGGACTGGCGCCCCTTCCCGGTGAAggg                                                                                                                                              >  7:107933/1‑149 (MQ=255)
   aaGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAgggg                                                                                                                                             <  5:292663/149‑1 (MQ=255)
   aaGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAgggg                                                                                                                                             <  3:368608/149‑1 (MQ=255)
    aGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAGGGGc                                                                                                                                            <  5:375986/149‑1 (MQ=255)
      accgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTTATTCCGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCtt                                                                                                                                          <  5:29681/149‑1 (MQ=255)
       ccgaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCAGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAGGGGcgcc                                                                                                                                         <  8:349131/149‑4 (MQ=255)
         gaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGTGCTTTTc                                                                                                                                       <  3:103039/149‑1 (MQ=255)
         gaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTc                                                                                                                                       <  3:54257/149‑1 (MQ=255)
         gaccTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCACCGGGAAGGGGcgccag                                                                                                                                       <  3:320388/149‑6 (MQ=255)
          accTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTcc                                                                                                                                      <  7:201793/149‑1 (MQ=255)
              ctcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTGACCAACCACAAGCTGACGATCGAGGAGAAGGACCTGGCCGACTGATTGAGCTGTTTTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCGACGc                                                                                                                                  >  3:284979/1‑149 (MQ=255)
              ctcGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGCCCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTGCCGGTGAAGGGGCTTTTCCACGc                                                                                                                                  >  3:289986/1‑149 (MQ=255)
                 ggCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCAGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGa                                                                                                                               >  3:259892/1‑149 (MQ=255)
                     cgcgAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCa                                                                                                                           >  2:243810/1‑149 (MQ=255)
                     cgcgAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCa                                                                                                                           >  1:336897/1‑149 (MQ=255)
                      gcgAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCAc                                                                                                                          <  8:93061/149‑1 (MQ=255)
                      gcgAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCAc                                                                                                                          >  7:323239/1‑149 (MQ=255)
                        gAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCt                                                                                                                        <  5:87179/149‑1 (MQ=255)
                         aaaTCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTg                                                                                                                       <  8:299526/149‑1 (MQ=255)
                          aaTCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGc                                                                                                                      <  6:269828/149‑1 (MQ=255)
                              gCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGCCCATCGAGGAGCAGGACCTGGCCGACTGATACCGCTGTTCTGGCGCCCCTGCCCGGTGAAGGGGCTTTTCCAGGCTGACTCACCTGccc                                                                                                                    >  4:44761/1‑147 (MQ=255)
                                 gagaCCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGa                                                                                                               <  8:323239/149‑1 (MQ=255)
                                      cGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTa                                                                                                          >  3:193453/1‑149 (MQ=255)
                                       gCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTAt                                                                                                         >  5:341535/1‑149 (MQ=255)
                                        cccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTAtt                                                                                                        >  5:96016/1‑149 (MQ=255)
                                         ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTAtt                                                                                                        >  6:188154/1‑148 (MQ=255)
                                         ccTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTAtt                                                                                                        >  1:139600/1‑148 (MQ=255)
                                            gAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCGTCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCt                                                                                                                        <  6:275622/129‑1 (MQ=255)
                                            gAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCGTCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCt                                                                                                                        >  5:275622/1‑129 (MQ=255)
                                            gAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATc                                                                                                    <  4:243543/149‑1 (MQ=255)
                                              aCATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGt                                                                                                           <  6:164112/140‑1 (MQ=255)
                                              aCATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGt                                                                                                           >  5:164112/1‑140 (MQ=255)
                                              aCATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCAt                                                                                                  <  6:96016/149‑1 (MQ=255)
                                               cATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATg                                                                                                 >  3:351864/1‑149 (MQ=255)
                                                aTCGCCGGCGATATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGt                                                                                                <  1:315191/149‑1 (MQ=255)
                                                  cGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTcc                                                                                              <  4:193453/149‑1 (MQ=255)
                                                  cGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTc                                                                                               >  4:345650/1‑148 (MQ=255)
                                                    ccGTCGACATATGCGTGTTCACCAACCACAACCTCAACATCGAGGATCAGGACCTGGCCGACTGATTAAGAAGTACTGGCGCCCCTTCACGCTGAATGGTATTATCCACGCTCCCTCACCTCCACAAGGACCGTATTGATAAAGTcc                                                                                              >  2:130015/1‑147 (MQ=255)
                                                          aCATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGTACCTGGCCGACTGATTCAGCTCTTCTGGCGCCCCTTCCCGGTGCAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGAcccc                                                                                      <  8:317195/149‑1 (MQ=255)
                                                                gCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGGGATGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgc                                                                                  >  8:318837/1‑147 (MQ=255)
                                                                 cGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgc                                                                                  <  2:139600/146‑1 (MQ=255)
                                                                   tgtTGACCAACCACAACCTTACCATCGAGGAGCTGGACCTGGCCGTCTGCTTCAGCTGTTCTGGCGCCGCTTGCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTTTTGATCATGTCCATGACCCCCCGCGAGAt                                                                             <  8:107933/149‑1 (MQ=255)
                                                                    gtTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAACGACCGTATTGATCATGTCCATGACCCCCCGCGAGATc                                                                            <  2:11542/149‑1 (MQ=255)
                                                                     ttCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATc                                                                            <  4:351864/148‑1 (MQ=255)
                                                                       cACCAACCACTACCTGACCATCGAGGAGCAGGACCTTGCCGACTGATTCAGCTGTTCTGGCGCCCCTTGCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGt                                                                          <  1:25168/148‑1 (MQ=255)
                                                                        accaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTc                                                                         >  6:191901/1‑148 (MQ=255)
                                                                        accaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGGGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTc                                                                         >  2:144515/1‑148 (MQ=255)
                                                                         ccaaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCACCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCa                                                                       >  7:346292/1‑149 (MQ=255)
                                                                          caaccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCAc                                                                      >  2:86217/1‑149 (MQ=255)
                                                                             ccaCAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGaa                                                                   <  5:188154/149‑1 (MQ=255)
                                                                                    cTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCCCGAGATCGTCCACGAACTCAAcc                                                            >  3:39727/1‑149 (MQ=255)
                                                                                         cATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCca                                                        >  4:257811/1‑148 (MQ=255)
                                                                                          aTCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCcaca                                                      >  2:300139/1‑149 (MQ=255)
                                                                                             gaggagCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCAcatc                                                    >  6:353766/1‑148 (MQ=255)
                                                                                              aggagCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCCcatcat                                                  <  7:318837/149‑1 (MQ=255)
                                                                                                gagCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCg                                                <  1:300139/149‑1 (MQ=255)
                                                                                                     ggACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGTCTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAg                                           <  2:116908/149‑1 (MQ=255)
                                                                                                      gACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATAGGCCAg                                           >  1:127354/1‑148 (MQ=255)
                                                                                                            gCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGgacgac                                     <  6:341535/148‑1 (MQ=255)
                                                                                                            gCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGacgacg                                    >  5:364220/1‑149 (MQ=255)
                                                                                                                aCTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCaa                                <  6:364220/149‑1 (MQ=255)
                                                                                                                    aTTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAgcgc                            >  5:177132/1‑149 (MQ=255)
                                                                                                                        aGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGc                                   <  7:340331/138‑1 (MQ=255)
                                                                                                                        aGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCg                        >  3:111231/1‑149 (MQ=255)
                                                                                                                        aGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCAACGAACTCAACCGCCACATCATCGGCCAGGACGACGc                                   >  8:340331/1‑138 (MQ=255)
                                                                                                                         gCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCaa                                >  8:348067/1‑140 (MQ=255)
                                                                                                                         gCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCaa                                <  7:348067/140‑1 (MQ=255)
                                                                                                                           tGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgc                                                                                  <  4:226847/88‑1 (MQ=255)
                                                                                                                           tGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCcgc                                                                                  >  3:226847/1‑88 (MQ=255)
                                                                                                                            gTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGc                    >  4:359636/1‑149 (MQ=255)
                                                                                                                             ttCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCCCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGc                    >  8:224324/1‑148 (MQ=255)
                                                                                                                               cTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCa                  <  1:173664/148‑1 (MQ=255)
                                                                                                                                    gcCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGccc            >  5:24371/1‑149 (MQ=255)
                                                                                                                                     ccccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCt           <  7:224324/149‑1 (MQ=255)
                                                                                                                                       ccatCCCGGTGACGGGGGTTTTCCACGCTGACTCACCTGCCCAAGGTCCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATAGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         <  4:284979/146‑1 (MQ=255)
                                                                                                                                       ccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCGACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         <  1:86217/149‑1 (MQ=255)
                                                                                                                                       ccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         <  5:353766/149‑1 (MQ=255)
                                                                                                                                       ccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         >  3:243315/1‑149 (MQ=255)
                                                                                                                                       ccTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         >  5:269975/1‑149 (MQ=255)
                                                                                                                                       ccTGCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCTATATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTgc         <  4:289986/149‑1 (MQ=255)
                                                                                                                                            ccGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAAc    <  8:346292/149‑1 (MQ=255)
                                                                                                                                             cGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACACCAAGCGCGCCGTGGCCAt                 >  7:78570/1‑135 (MQ=255)
                                                                                                                                             cGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACACCAAGCGCGCCGTGGCCAt                 <  8:78570/135‑1 (MQ=255)
                                                                                                                                              ggTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAACCg  <  1:144515/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AACAAGACCGACCTCTCGGCCCGCGAAATCGCCGAGACCGCCCTGAACATCGCCGGCGACATCTGCGTGTTCACCAACCACAACCTGACCATCGAGGAGCAGGACCTGGCCGACTGATTCAGCTGTTCTGGCGCCCCTTCCCGGTGAAGGGGCTTTTCCACGCTGACTCACCTGCCCAAGGACCGTATTGATCATGTCCATGACCCCCCGCGAGATCGTCCACGAACTCAACCGCCACATCATCGGCCAGGACGACGCCAAGCGCGCCGTGGCCATTGCCCTGCGCAACCG  >  NC_002947/5697174‑5697464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑