Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,701,222 C→G 9.2% intergenic (+20/‑43) phaA → / → phaB poly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,701,2220CG 9.2% 191.4 / 9.2 65intergenic (+20/‑43)phaA/phaBpoly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base G (3/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

GTTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGAT  >  NC_002947/5701079‑5701362
                                                                                                                                               |                                                                                                                                            
gtTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCa                                                                                                                                          <  1:322963/148‑1 (MQ=255)
    cACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGgc                                                                                                                                      <  6:286854/148‑1 (MQ=255)
      cTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGCAGGCCGCCACGgcgct                                                                                                                                   <  6:178661/149‑2 (MQ=255)
      cTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGCAGGCCGCCACGgcgct                                                                                                                                   <  7:211228/149‑2 (MQ=255)
         gCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACg                                                                                                                                >  1:296743/1‑149 (MQ=255)
         gCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc                                                                                                                                 <  2:113719/148‑1 (MQ=255)
           agagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCAGCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgt                                                                                                                              >  2:206560/1‑149 (MQ=255)
           agagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCACggc                                                                                                                              <  6:113903/149‑2 (MQ=255)
            gagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGAGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtg                                                                                                                             <  8:76820/149‑1 (MQ=255)
                  gCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACt                                                                                                                       >  5:381788/1‑149 (MQ=255)
                    tGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACtt                                                                                                                      >  8:226396/1‑148 (MQ=255)
                      ggCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc                                                                                                                   <  4:18226/149‑1 (MQ=255)
                         gAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTAc                                                                                                                        <  1:179975/141‑1 (MQ=255)
                          aGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa                                                                                                               <  7:270865/149‑1 (MQ=255)
                            cGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAaga                                                                                                             <  8:154817/149‑1 (MQ=255)
                            cGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAaga                                                                                                             <  6:381788/149‑1 (MQ=255)
                              tGCCGGCGCGCTGAAAACGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGt                                                                                                           <  2:307010/149‑1 (MQ=255)
                                ccGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCATCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTc                                                                                                          <  1:206560/148‑1 (MQ=255)
                                 cGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCAc                                                                                                        <  7:226396/149‑1 (MQ=255)
                                 cGGCGCGCTGAAAAAGGCACCGACCCGCCAGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGAGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCAc                                                                                                        >  2:73126/1‑149 (MQ=255)
                                       gcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCAt                                                                                                  >  1:342338/1‑149 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAccc                                                                                                                 <  2:100857/132‑1 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAccc                                                                                                                 >  1:100857/1‑132 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGc                                                                                                >  2:171493/1‑149 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCACg                                                                                                                                <  2:239362/117‑1 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCACg                                                                                                                                >  1:239362/1‑117 (MQ=255)
                                         tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATACCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGc                                                                                                >  1:211493/1‑149 (MQ=255)
                                                 gCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTAACCCAAGAGTCACGTGCATGCCGCAAccc                                                                                        >  4:90791/1‑149 (MQ=255)
                                                    aagACCCTCCTGGGCAAACGTACCTATGCCGCCGGCTAAGCCTCCCCAGGCACCAACGTTCACGATCGTTGAGTTACCGCGCCGTGGAGGCCTTAACGGCGCCACGGTGTTTACTTAAACCAAGAGTCACGTTCATGCAGCAAACCta                                                                                      >  4:251344/3‑148 (MQ=255)
                                                       aCCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGc                                                                                             <  6:256719/138‑1 (MQ=255)
                                                       aCCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGc                                                                                             >  5:256719/1‑138 (MQ=255)
                                                           gCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCa                                                                              <  2:296743/149‑1 (MQ=255)
                                                            ccTGGGCAACCGTACCTATGGCGCCGGCGAAGCCTCCGCAGGCACCTACGTTCTCGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTGACGTGCATGCCGCAACCCTATATTTTCAg                                                                             <  8:188914/149‑1 (MQ=255)
                                                                 gCAACCGTACCTATGCCGCCGGCGAAGCCTTCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCg                                                                        >  4:196468/1‑149 (MQ=255)
                                                                   aaCCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCAGCAACCCTATATTTTCAGGACCGTc                                                                      >  3:10330/1‑149 (MQ=255)
                                                                     ccGTACCTATACCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGa                                                                    <  2:211493/149‑1 (MQ=255)
                                                                          ccTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg                                                                <  7:261574/148‑1 (MQ=255)
                                                                          ccTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg                                                                <  2:334552/148‑1 (MQ=255)
                                                                             aTGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGAc                                                             >  8:195779/1‑148 (MQ=255)
                                                                                ccgccgGCGAAGCCTTCCCAGGCACCTACGTTCACGAGCGTTGAGGTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAAc                                                          <  3:196468/148‑1 (MQ=255)
                                                                                ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAAc                                                          <  7:195779/148‑1 (MQ=255)
                                                                                ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAAc                                                          <  1:171493/148‑1 (MQ=255)
                                                                                 cgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCg                                                                        <  1:232499/133‑1 (MQ=255)
                                                                                 cgccgGCGAAGCCTACCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCg                                                                        >  2:232499/1‑133 (MQ=255)
                                                                                      gCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCa                                                   >  7:120333/1‑149 (MQ=255)
                                                                                        gAAGCCTCCCCAGGCAGCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGGCGCAACCCTATATTTTCAGGACCTTCGAGCTGGACATCCAGTACATc                                                 >  8:151086/1‑149 (MQ=255)
                                                                                             cTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCAc                                            <  1:276527/149‑1 (MQ=255)
                                                                                               ccccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCg                                          >  2:287226/1‑149 (MQ=255)
                                                                                                cccAGGCCCCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGAGTTTACTTAACCCAAGAGTCACGTGAATGACGCAACCCGATATTTTCAGGACAGTCGAGCTGGACAACCAGTCCATCCGCACCGc                                         >  8:235032/1‑149 (MQ=255)
                                                                                                cccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGACACGGTGTTTACTTCACCCAAGAGTAACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGc                                         >  7:53236/1‑149 (MQ=255)
                                                                                                 ccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCt                                        >  1:47715/1‑149 (MQ=255)
                                                                                                 ccAGGCACCTACGATCACGAGCGTGGAGTTCCAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCt                                        <  2:40993/149‑1 (MQ=255)
                                                                                                       aCCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGc                                  >  3:34539/1‑149 (MQ=255)
                                                                                                                 aCGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAg                         <  1:357843/148‑1 (MQ=255)
                                                                                                                 aCGACCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGATTCACGTGCAAGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAg                         <  7:151086/148‑1 (MQ=255)
                                                                                                                   gAGCGTTGAGATACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTGACCCAAGATTCACGTGCATGCCGCAACCCTTTATTTTCATGACCTTCGAGCTGGACAACCAGTCCATCCTCCTCGCTGTTCGCCCCGGCAAGCCg                      <  7:235032/149‑1 (MQ=255)
                                                                                                                      cGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCAc                   <  1:287226/149‑1 (MQ=255)
                                                                                                                        ttGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCt                                                                 >  5:360327/1‑101 (MQ=255)
                                                                                                                        ttGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCt                                                                 <  6:360327/101‑1 (MQ=255)
                                                                                                                        ttGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCt                 <  4:34539/149‑1 (MQ=255)
                                                                                                                         tGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCt                 >  6:270626/1‑148 (MQ=255)
                                                                                                                              gtaaacaccGTGGCGCCGTGCAGGCCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGCCAACCAGTCCCTCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGcc           >  5:357152/8‑149 (MQ=255)
                                                                                                                                 aacaccGTGGCGCCGTGCAGGCCGCCACGGCGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGtt        >  8:58834/5‑149 (MQ=255)
                                                                                                                                 aGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGtt        >  8:144038/1‑149 (MQ=255)
                                                                                                                                       gTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGAt  >  1:281925/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                            
GTTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAGAGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGAT  >  NC_002947/5701079‑5701362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑