Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,457 G→A 12.5% intergenic (+22/+49) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4570GA12.5% 99.3 / 4.5 40intergenic (+22/+49)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (12/23);  new base A (2/3);  total (14/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAC  >  NC_002947/5740311‑5740600
                                                                                                                                                  |                                                                                                                                               
gTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGcc                                                                                                                                               <  3:235056/149‑1 (MQ=255)
    cGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTc                                                                                                                                           <  2:191801/149‑1 (MQ=255)
     gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcg                                                                                                                                          <  7:39046/149‑1 (MQ=255)
     gATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcg                                                                                                                                          <  3:106740/149‑1 (MQ=255)
      aTCGCCAGGGGCGCGATGTGATGGGGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcgc                                                                                                                                         <  3:192669/149‑1 (MQ=255)
       tCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATagcg                                                                                                                                        >  5:178253/1‑149 (MQ=255)
          ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTg                                                                                                                                      >  3:213381/1‑148 (MQ=255)
            aGGTGCGCGATGTGATGGTGAATGGGCAATGGCTGGTGCGCAAGGGGCGGCATGCCCAGGAAGATGAAAGCCGGCGGGAGTTTGCCAAGGTGTTGCGCCAGATTATGGGCTGAGCGTACCGGGGTTGCGTTGCAACCATTCGCGGGctt                                                                                                                                   >  7:77001/1‑147 (MQ=255)
                        tgATGGTGAATGGGGATTGGGTGGTGCGCGAGGGGCGGCATTCCCAGGAAGATGAATGCGGGCGGGCGTTTGCCAAGGTGTTGCTCCAGCTTTTGTGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAggg                                                                                                                       <  8:77001/149‑1 (MQ=37)
                        tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAggg                                                                                                                       <  4:213381/149‑1 (MQ=255)
                          aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                     >  6:49937/1‑149 (MQ=255)
                          aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                     <  5:288166/149‑1 (MQ=255)
                          aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGt                                                                                                                      <  4:14742/148‑1 (MQ=255)
                           tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTg                                                                                                                    <  5:49937/149‑1 (MQ=255)
                             gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGGGGTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTGCGCGGGCTTGCACGCGAAGGGCTgc                                                                                                                   <  5:28292/148‑1 (MQ=37)
                              tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaa                                                                                                                 <  2:352266/149‑1 (MQ=255)
                                                    ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcgc                                                                                                                                         >  1:266583/1‑103 (MQ=255)
                                                    ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTcgc                                                                                                                                         <  2:266583/103‑1 (MQ=255)
                                                        gggCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATCGCGGGCAAGCCCGCGCAGGGTTGCAACGCACACCCAAAAAACTCAGTGCAtt                                                                                       >  7:280807/1‑149 (MQ=255)
                                                            ggcATGCCCTGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCTTACCGGGGTTGCTTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                   <  7:30910/149‑1 (MQ=255)
                                                                  cccAGGACTATGACAGCGGGCGGGCGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGTTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                             <  4:251735/149‑1 (MQ=255)
                                                                  cccAGGAAGATGACACCGGGCGGTCTTTTGCCAAGGTGTTGCGCCAGGTGTTGGGCTGAGCGTACCGGGGTTGCGTTCCAGCCCTTGGCTTGCAAGCCCGCCAAGTGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAtt                                                                              <  2:17746/148‑1 (MQ=255)
                                                                  cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCACGGGGTGGCGCCAGCTTTTGGGCTGCGCGAACCGGGGTGCCGTTGCAGCCCTCCGCGGGCAAGCCCGCTAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                             <  6:331661/149‑1 (MQ=255)
                                                                  cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGt                                                                                                                                       <  5:378416/91‑1 (MQ=255)
                                                                  cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGt                                                                                                                                       >  6:378416/1‑91 (MQ=255)
                                                                               aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcg                                                                 <  3:150714/148‑1 (MQ=255)
                                                                                    ggcgggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGGTGGCGCCCATAGCGGGGAAGCCCCCGAACGGTTGGAACGCCACCCCCAAAAACACAGGGGAGTTTTTATATTTTGAGATGGGGcgcccccc                                                           >  4:205720/1‑149 (MQ=255)
                                                                                                              cAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGa                                                                                                                           <  7:178473/59‑1 (MQ=255)
                                                                                                              cAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGa                                                                                                                           >  8:178473/1‑59 (MQ=255)
                                                                                                                     tGGGCTGAGCGTACCGGGGTGGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCtgctgc                           <  1:221183/148‑1 (MQ=255)
                                                                                                                      ggtcTGCGCGTACGGGGGTTGCGTGCCAGCCATGCGGGTGCAACCCCGCTATGGTTTCCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                          <  8:207520/145‑1 (MQ=255)
                                                                                                                          tGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                      <  7:230331/100‑1 (MQ=255)
                                                                                                                                   cGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc            <  4:263287/149‑1 (MQ=255)
                                                                                                                                      ggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCAGCTGCCGCGCCCTGGACAGCAgg         >  2:171233/1‑149 (MQ=255)
                                                                                                                                       gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt        >  2:138931/1‑149 (MQ=255)
                                                                                                                                        ttgcgttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt       >  5:278440/1‑149 (MQ=37)
                                                                                                                                          gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc      >  4:15642/1‑148 (MQ=255)
                                                                                                                                          gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc      <  3:205720/148‑1 (MQ=255)
                                                                                                                                            gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa   <  7:161900/149‑1 (MQ=255)
                                                                                                                                             ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc  >  6:239880/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTGGCGATCGCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAC  >  NC_002947/5740311‑5740600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑