Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,484 T→C 13.5% intergenic (+49/+22) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4840TC13.5% 84.9 / 6.2 37intergenic (+49/+22)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (14/18);  new base C (2/3);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

ATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGC  >  NC_002947/5740337‑5740629
                                                                                                                                                   |                                                                                                                                                 
aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                                  >  6:49937/1‑149 (MQ=255)
aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                                  <  5:288166/149‑1 (MQ=255)
aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGt                                                                                                                                                   <  4:14742/148‑1 (MQ=255)
 tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTg                                                                                                                                                 <  5:49937/149‑1 (MQ=255)
   gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGGGGTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTGCGCGGGCTTGCACGCGAAGGGCTgc                                                                                                                                                <  5:28292/148‑1 (MQ=37)
    tGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaa                                                                                                                                              <  2:352266/149‑1 (MQ=255)
                              gggCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCATCGCGGGCAAGCCCGCGCAGGGTTGCAACGCACACCCAAAAAACTCAGTGCAtt                                                                                                                    >  7:280807/1‑149 (MQ=255)
                                  ggcATGCCCTGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCTTACCGGGGTTGCTTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                                                <  7:30910/149‑1 (MQ=255)
                                        cccAGGACTATGACAGCGGGCGGGCGTTTGCCATGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGTTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                                                          <  4:251735/149‑1 (MQ=255)
                                        cccAGGAAGATGACACCGGGCGGTCTTTTGCCAAGGTGTTGCGCCAGGTGTTGGGCTGAGCGTACCGGGGTTGCGTTCCAGCCCTTGGCTTGCAAGCCCGCCAAGTGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAtt                                                                                                           <  2:17746/148‑1 (MQ=255)
                                        cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCACGGGGTGGCGCCAGCTTTTGGGCTGCGCGAACCGGGGTGCCGTTGCAGCCCTCCGCGGGCAAGCCCGCTAAGGGTGGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAttt                                                                                                          <  6:331661/149‑1 (MQ=255)
                                                     aaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcg                                                                                              <  3:150714/148‑1 (MQ=255)
                                                          ggcgggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGGTGGCGCCCATAGCGGGGAAGCCCCCGAACGGTTGGAACGCCACCCCCAAAAACACAGGGGAGTTTTTATATTTTGAGATGGGGcgcccccc                                                                                        >  4:205720/1‑149 (MQ=255)
                                                                                           tGGGCTGAGCGTACCGGGGTGGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCtgctgc                                                        <  1:221183/148‑1 (MQ=255)
                                                                                            ggtcTGCGCGTACGGGGGTTGCGTGCCAGCCATGCGGGTGCAACCCCGCTATGGTTTCCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGcc                                                       <  8:207520/145‑1 (MQ=255)
                                                                                                tGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                                   <  7:230331/100‑1 (MQ=255)
                                                                                                         cGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                         <  4:263287/149‑1 (MQ=255)
                                                                                                            ggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCAGCTGCCGCGCCCTGGACAGCAgg                                      >  2:171233/1‑149 (MQ=255)
                                                                                                             gttgcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGt                                     >  2:138931/1‑149 (MQ=255)
                                                                                                              ttgcgttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt                                    >  5:278440/1‑149 (MQ=37)
                                                                                                                gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                                   >  4:15642/1‑148 (MQ=255)
                                                                                                                gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTtc                                   <  3:205720/148‑1 (MQ=255)
                                                                                                                  gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                                <  7:161900/149‑1 (MQ=255)
                                                                                                                   ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                               >  6:239880/1‑149 (MQ=255)
                                                                                                                       aacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCGAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc                           >  6:27733/3‑149 (MQ=38)
                                                                                                                         cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                             <  2:165964/129‑1 (MQ=255)
                                                                                                                         cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                             >  1:165964/1‑129 (MQ=255)
                                                                                                                          ccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                          >  1:24685/1‑147 (MQ=255)
                                                                                                                           cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg                       >  3:369949/1‑149 (MQ=255)
                                                                                                                            ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg                      >  3:297997/1‑149 (MQ=255)
                                                                                                                                cgTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg                  <  7:104094/149‑1 (MQ=255)
                                                                                                                                       gCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTc           >  3:62217/1‑149 (MQ=255)
                                                                                                                                       gCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTc           >  6:297208/1‑149 (MQ=255)
                                                                                                                                        cccGCGAATGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCg          <  5:358793/149‑1 (MQ=255)
                                                                                                                                          cgcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt        >  3:164348/1‑149 (MQ=255)
                                                                                                                                          cgcgAAGGGCTGCAACGCAACCCCGAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt        <  5:27733/149‑1 (MQ=255)
                                                                                                                                          cgcgAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt        <  6:278440/149‑1 (MQ=255)
                                                                                                                                           gcgAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCAGCGCCCTGGACAGCAGGTTCTCACGATCCCAGGCCGGCAGTGTCGgtg       >  7:355918/1‑149 (MQ=255)
                                                                                                                                                gggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc  <  3:15642/149‑1 (MQ=255)
                                                                                                                                                gggTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTgcgc  <  4:164348/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGTGTGCGC  >  NC_002947/5740337‑5740629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑