Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,750,790	T→A	5.7%	D258V (GAC→GTC)	thiI ←	tRNA sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,750,790	0	T	A	5.7%	201.0 / 2.6	70	D258V (GAC→GTC)	thiI	tRNA sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base A (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

GTCGATGATCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGTTGAAG  >  NC_002947/5750642‑5750933                                                                                                                                                     |                                                                                                                                                gTCGATGATCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCtgttgt                                                                                                                                                 >  1:192320/1‑149 (MQ=255)    gatcatCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGa                                                                                                                                              >  3:2756/1‑149 (MQ=255)     atgatCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCGTGAGGGTCACGCCCATGTAGCTGTTGTCGAc                                                                                                                                             >  8:162589/1‑149 (MQ=255)        atCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCtt                                                                                                                                          <  1:325091/149‑1 (MQ=255)           gACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgtt                                                                                                                                       <  1:273231/149‑1 (MQ=255)             cAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgtt                                                                                                                                       >  6:271677/1‑147 (MQ=255)               ggTTCGGCAGGGTCTGTCTGGACACCTGGGAACTCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAgg                                                                                                                                   <  2:319601/149‑1 (MQ=255)               ggTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAgg                                                                                                                                   <  2:192320/149‑1 (MQ=255)                        gggTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGcc                                                                                                                           >  7:204921/1‑148 (MQ=255)                           tCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGAGCTCGCCAAcc                                                                                                                       >  4:250920/1‑149 (MQ=255)                               gctggACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGGCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCActt                                                                                                                   <  1:295653/149‑1 (MQ=255)                                 tggACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAAGCGGTCGGCCATATGGGCGGCGCCGCGCAGCAACATGCGCATGAGGGTCAAGCCCATGTAGCTGTTGTCCACCATGTTGAGGATCACGCCAACCAcataa                                                                                                                 >  1:275618/1‑145 (MQ=255)                                   gACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAAGCActtctt                                                                                                                <  4:2756/148‑1 (MQ=255)                                   gACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACttcttc                                                                                                               <  3:282183/149‑1 (MQ=255)                                        cTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgt                                                                                                                                        <  1:351816/119‑1 (MQ=255)                                        cTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgt                                                                                                                                        >  2:351816/1‑119 (MQ=255)                                        cTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCACCCACTTCTTCGAACg                                                                                                          >  3:301692/1‑149 (MQ=255)                                        cTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACg                                                                                                          >  7:304944/1‑149 (MQ=255)                                            gAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGTAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCAc                                                                                                      <  6:173699/149‑1 (MQ=255)                                                tCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCt                                                                                                   >  6:316028/1‑148 (MQ=255)                                                tCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCt                                                                                                   >  6:371941/1‑148 (MQ=255)                                                   cTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCtgatg                                                                                               <  8:304944/149‑1 (MQ=255)                                                       gCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACa                                                                                           <  3:82664/149‑1 (MQ=255)                                                       gCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACa                                                                                           >  3:60616/1‑149 (MQ=255)                                                        ccGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGACGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAg                                                                                          >  3:125644/1‑149 (MQ=255)                                                        ccGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGACGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAg                                                                                          >  3:131040/1‑149 (MQ=255)                                                         cGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAg                                                                                          >  2:356426/1‑148 (MQ=255)                                                         cGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGt                                                                                         <  7:81243/149‑1 (MQ=255)                                                          ggTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTa                                                                                        >  2:288305/1‑149 (MQ=255)                                                          ggTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTa                                                                                        >  2:110164/1‑149 (MQ=255)                                                               ccAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgtt                                                                                                                                       <  6:21536/97‑1 (MQ=255)                                                               ccAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCttgtt                                                                                                                                       >  5:21536/1‑97 (MQ=255)                                                               ccAGCGCGTCAAACTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGAGCTTGAGGGTCACGCCCAAGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCAAACCACTTCTTCGAACGGCACGCTGATGAAAAGTACgcgc                                                                                   >  7:135967/1‑149 (MQ=255)                                                                cAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCgct                                                                                  >  6:382688/1‑149 (MQ=255)                                                                   cgcgTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCgctggc                                                                               <  5:51800/149‑1 (MQ=255)                                                                      gTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGgctgc                                                                            >  1:202053/1‑149 (MQ=255)                                                                           tCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGGTTGAGGGTCACGCCCATGTTGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCg                                                                       >  7:277959/1‑149 (MQ=255)                                                                           tCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCAGGCTGCTGCCg                                                                       >  4:319231/1‑149 (MQ=255)                                                                                    ggTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTc                                                              <  5:371941/149‑1 (MQ=255)                                                                                    ggTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTc                                                              <  8:40948/149‑1 (MQ=255)                                                                                    ggTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTc                                                              <  1:356426/149‑1 (MQ=255)                                                                                        ggCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCca                                                            >  8:150113/1‑147 (MQ=255)                                                                                            atatGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGt                                                       >  8:138288/1‑148 (MQ=255)                                                                                             tatGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTaa                                                     >  2:370994/1‑149 (MQ=255)                                                                                                 ggcggcGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATgg                                                  <  4:60616/148‑1 (MQ=255)                                                                                                 ggcggcGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATgg                                                  <  5:316028/148‑1 (MQ=255)                                                                                                 ggcggcGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATgg                                                  <  2:275618/148‑1 (MQ=255)                                                                                                  gcggcgCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCCGGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGc                                                >  5:124524/1‑149 (MQ=255)                                                                                                  gcggcgCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATggca                                                >  8:308889/1‑147 (MQ=255)                                                                                                     gcgcCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCAc                                             <  5:135867/149‑1 (MQ=255)                                                                                                     gcgcCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCAc                                             >  2:169241/1‑149 (MQ=255)                                                                                                       gccgcGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACtt                                           <  1:288305/149‑1 (MQ=255)                                                                                                         cgcgCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTcc                                         <  5:271677/149‑1 (MQ=255)                                                                                                          gcgcAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCa                                        <  4:301692/149‑1 (MQ=255)                                                                                                               gcaTCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACg                                   <  2:37303/149‑1 (MQ=255)                                                                                                                    atGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAg                              >  4:120924/1‑149 (MQ=255)                                                                                                                    atGCGCTTGAGGGTCACGCACATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAg                              >  2:230456/1‑149 (MQ=255)                                                                                                                            gAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTgg                       <  7:162589/148‑1 (MQ=255)                                                                                                                            gAGGGTCACGCCCATGTAGCTGTTGACGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTgg                       <  4:131040/148‑1 (MQ=255)                                                                                                                            gAGGGTCACGCCCATGTAGCTGTTGACGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTgg                       <  4:125644/148‑1 (MQ=255)                                                                                                                              gggTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCa                    <  2:183289/149‑1 (MQ=255)                                                                                                                               ggTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCAc                   >  6:175448/1‑149 (MQ=255)                                                                                                                               ggTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCAc                   <  3:319231/149‑1 (MQ=255)                                                                                                                                   aCGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAGCCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTGCACAGGTAATGGGCCACTGCGATTACGCCCAGCTCGTGGGCACGgcg               >  4:129109/1‑148 (MQ=255)                                                                                                                                     gCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGgccgc             >  4:159698/1‑149 (MQ=255)                                                                                                                                       ccATGTAGCTGTTGTTGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCAGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACACCCAGCTCGTGGGCACGgccgcc            >  1:119154/1‑148 (MQ=255)                                                                                                                                       ccATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGgccgcc            >  1:320979/1‑148 (MQ=255)                                                                                                                                         aTGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAg         <  3:120924/149‑1 (MQ=255)                                                                                                                                          tGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAgg        <  5:28837/149‑1 (MQ=255)                                                                                                                                          tGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAgg        <  1:370994/149‑1 (MQ=255)                                                                                                                                            tAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGtt      <  5:382688/149‑1 (MQ=255)                                                                                                                                                tgttgtCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGTTg     >  6:89895/1‑146 (MQ=255)                                                                                                                                                  ttgtCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGTTgaag  >  8:163449/1‑147 (MQ=255)                                                                                                                                                  ttgtCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGTTgaag  <  1:230456/147‑1 (MQ=255)                                                                                                                                                    gtCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCa                    >  7:333417/1‑127 (MQ=255)                                                                                                                                                     |                                                                                                                                                GTCGATGATCGACAGGTTCGGCAGGGTCTGGCTGGACACCTGGGAAATCGCTTCGCCGGTCACCAGCGCGTCAATCTGCAGGCGGTCGGCCATATGGGCGGCGCCGCGCAGCATCATGCGCTTGAGGGTCACGCCCATGTAGCTGTTGTCGACCTTGTTGAGGATCTCGCCAACCACTTCTTCGAACGGCACGCTGATGAACAGTACGCGCTGGCTGCTGCCGTATTTTTTCCACAGGTAATGGGCCACTTCCATTACGCCCAGCTCGTGGGCACGGCCGCCGAGGTTGAAG  >  NC_002947/5750642‑5750933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑