| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,759,521 | A→G | 10.6% | intergenic (+130/+26) | trmL → / ← secB | tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,759,521 | 0 | A | G | 10.6% | 126.5 / 5.5 | 48 | intergenic (+130/+26) | trmL/secB | tRNA (cytosine(34)‑2'‑O)‑methyltransferase TrmL/protein‑export protein SecB |
| Reads supporting (aligned to +/- strand): ref base A (28/14); new base G (3/2); total (32/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.03e-01 | |||||||||||
GTTTGCCGGGAGCTGATGTGTTGCCTGTGCGGGCCTCTTCGCGGGTAAACCCGCTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGAACCGCGGGTCACCAGGCTG > NC_002947/5759376‑5759667 | gTTTGCCGGGAGCTGATGTGTTGCCTGTGCGGGCCTCTTCGCGGGTAAACCCGCTCCTACGCAGGCCGAGGGCACGGCGCCAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGgc > 2:244286/1‑149 (MQ=255) ccGGGAGCTGATGTGTTGCCTGTGCGGGCCTCTTCGCGGGTAAACCCGCTACTAAGCAGGCCGAGGGAACGGCGCAAT‑ACCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCtt > 2:30962/1‑148 (MQ=255) tgtggtgtTGCCTGGGCGGCCCTCTGCGCGGGAAACCCCGCTCCTCCGCCGCCCGCGGCACCGGCGAAAC‑CCCCTGTCGGAGGGGTTTAACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAAc < 6:162164/145‑1 (MQ=255) tGATGTGTTGCCTGTGCGGGCCTCTTCGCGGGTAAAACCCCGGCGGCGCAGGGCCAGGGGACCGCGCGATACCCGGGTGGGGGGGGGTTTACCC‑CGCGGGGGCCCGCGCCCGGCAA‑AAAAAAACCCCCCGCGACGCGGGTTGATGAAAc > 4:82625/1‑149 (MQ=255) ttGCCTGTGCGGGCCTCTTCGCGGGTTAACCCGCGCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTTCCCGCGAAGAGGCCCCCG‑CCGGGAACAAAAAAGCGCCCCGAGCGGCGCGTTGTTGAAACCGGCAg > 6:280634/1‑148 (MQ=255) ctctTCGCGGGTAAACCCGCTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCCGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAg > 6:279236/1‑148 (MQ=255) gggTAAACCCGCTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCtt > 7:269456/1‑149 (MQ=255) ccGCTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGAGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGAGCTTCA‑CCCGCt > 7:113503/1‑149 (MQ=255) cTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTcc < 5:279236/149‑1 (MQ=255) tCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCt > 6:240781/1‑149 (MQ=255) gCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCa > 1:169847/1‑107 (MQ=255) gCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCa < 2:169847/107‑1 (MQ=255) gCCGAGGGAACGGCGCAAT‑CCCCTGAAGGAGCGCGTAAACCCGCGAAGAGGCCCCCG‑CCCGCAACAAAAAAGCGCCCCGACAGGCGCTTACAACAAACCCGAAGAAATTACTTCACAGTAGGCGCTTCA‑CCAGCTTCCTGCATACGCTg > 4:125940/1‑149 (MQ=255) aaCGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCtt > 3:282271/1‑149 (MQ=255) gcAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCTCTCGGGGCGCTTTTTTg > 5:230122/1‑80 (MQ=21) gcAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCTCTCGGGGCGCTTTTTTg < 6:230122/80‑1 (MQ=21) aaT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTa > 1:46139/1‑148 (MQ=255) ccccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCACCCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAg < 1:244286/149‑1 (MQ=255) cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAggg > 2:367041/1‑149 (MQ=255) cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAgg < 5:247012/148‑1 (MQ=255) cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAgg > 6:247012/1‑148 (MQ=255) gTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTc < 4:118071/149‑1 (MQ=255) aGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCg > 4:8539/1‑148 (MQ=255) aGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGt > 2:228719/1‑149 (MQ=255) gCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGtt > 6:12500/1‑149 (MQ=255) gTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGAcc > 3:120135/1‑149 (MQ=255) ccGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGa > 1:268535/1‑148 (MQ=255) ccGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGa > 2:121760/1‑148 (MQ=255) ccGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGAc > 5:106622/1‑149 (MQ=255) cgcgAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGt < 1:98105/137‑1 (MQ=255) cgcgAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGt > 2:98105/1‑137 (MQ=255) gcg‑cCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGTCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCgg < 3:350449/149‑1 (MQ=255) ccggtttcaaGAAAGCGCCTCTCGGGGCGCTTTTTTGTTGCCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaa > 6:222249/8‑149 (MQ=37) caacaaAAAAGCGCCCCGAGAGGCGCTTTCTAGAAACCGCAAGTAATTACTGCACAGTAGGCGCTTAA‑CCCGCTACCTCCATACGCTGCATTTCTTGCGCGGACAGGGCGTAGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaa > 8:117986/1‑149 (MQ=255) aacaaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac > 6:59729/1‑149 (MQ=255) aacaaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac > 4:353261/1‑149 (MQ=255) aacaaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac < 4:120135/149‑1 (MQ=255) aacaaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac < 3:353261/149‑1 (MQ=255) aacaaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac < 2:268535/149‑1 (MQ=255) caaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac < 6:236025/147‑1 (MQ=255) caaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaacgaac > 5:236025/1‑147 (MQ=255) caaAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAAGTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGAACcg > 6:337387/1‑149 (MQ=255) gaaaGCGCCTCTCGGGGCGCTTTTTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑c > 1:126504/2‑63 (MQ=21) gaaaGCGCCTCTCGGGGCGCTTTTTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑c < 2:126504/62‑1 (MQ=21) aaaaGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGAACCGCgg < 1:228719/149‑1 (MQ=255) gcCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGCACCGCGGGTCAc > 4:254437/1‑148 (MQ=255) cccGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaa < 7:305217/131‑1 (MQ=255) cccGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGgaa > 8:305217/1‑131 (MQ=255) agagGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGAACCGCGGGTCACCAGGCTg < 5:280634/149‑1 (MQ=255) | GTTTGCCGGGAGCTGATGTGTTGCCTGTGCGGGCCTCTTCGCGGGTAAACCCGCTCCTACGCAGGCCGAGGGAACGGCGCAAT‑CCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGCG‑CCGGCAACAAAAAAGCGCCCCGAGAGGCGCTTTCTTGAAACCGGCAGTAATTACTGCACAGTAGGCGCTTCA‑CCCGCTTCCTGCATACGCTGCATTTCTTGCGCGTACAGGGCGTCGAAGTTGACCGGCGACAGCATCAGCGCCGGGAACGAACCGCGGGTCACCAGGCTG > NC_002947/5759376‑5759667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |