Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,797,312 G→T 8.9% R196R (CGC→CGA)  gltB ← L‑glutamate synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,797,3120GT 8.9% 204.9 / 9.0 67R196R (CGC→CGA) gltBL‑glutamate synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base T (4/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

GATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGG  >  NC_002947/5797168‑5797450
                                                                                                                                                |                                                                                                                                          
gATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATcgcgcg                                                                                                                                        >  3:297511/1‑149 (MQ=255)
gATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGGCTGGATAAAACGCCGCGAGATcgcgc                                                                                                                                         <  3:337285/148‑1 (MQ=255)
    tcGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcat                                                                                                                                    >  4:120312/1‑149 (MQ=255)
     cGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatc                                                                                                                                   <  5:48030/149‑1 (MQ=255)
      gCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatca                                                                                                                                  >  2:143587/1‑149 (MQ=255)
        cGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAGCCCGCAGCTCGCGGGGAGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatcat                                                                                                                                 >  1:308745/1‑148 (MQ=255)
             tgGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGcc                                                                                                                           >  1:281514/1‑149 (MQ=255)
                gCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTTGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACGCAGGTCTGGATAAAACTCCGCGAGATCGCGCGGCATCATCAGGCCttt                                                                                                                        <  5:189863/149‑1 (MQ=255)
                     gAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAga                                                                                                                   <  1:143587/149‑1 (MQ=255)
                     gAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAga                                                                                                                   <  6:142107/149‑1 (MQ=255)
                       aGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAgct                                                                                                                  >  3:25725/1‑146 (MQ=255)
                          ggAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatca                                                                                                                                  >  6:175795/1‑129 (MQ=255)
                          ggAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGcatca                                                                                                                                  <  5:175795/129‑1 (MQ=255)
                          ggAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGatgatg                                                                                                              <  3:10007/149‑1 (MQ=255)
                           gAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATgg                                                                                                             <  1:323960/149‑1 (MQ=255)
                             aCGTCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTc                                                                                                           <  5:89190/149‑1 (MQ=255)
                               ggCTGCGCCAGCGGCCATTTCGGCAGTGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCtt                                                                                                         <  6:282610/149‑1 (MQ=255)
                               ggCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCtt                                                                                                         <  4:297511/149‑1 (MQ=255)
                               ggCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCtt                                                                                                         >  7:343260/1‑149 (MQ=255)
                                 cTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTtgt                                                                                                       >  7:206888/1‑149 (MQ=255)
                                   gcgcCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGc                                                                                                     >  7:60048/1‑149 (MQ=255)
                                    cgcCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGc                                                                                                     >  8:73946/1‑148 (MQ=255)
                                            gCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTg                                                                                            <  8:206888/149‑1 (MQ=255)
                                              cATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATAGCGGTTTGCAGGCGATCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTTGATGATGGTCTTGTTCGAGAAGCTg                                                                                            >  4:336717/1‑147 (MQ=255)
                                               aTTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCTCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAg                                                                                         <  7:350330/149‑1 (MQ=255)
                                               aTTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAg                                                                                         <  8:60048/149‑1 (MQ=255)
                                                     gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAg                                                                                   >  4:70589/1‑149 (MQ=255)
                                                     gCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAg                                                                                   >  6:335789/1‑149 (MQ=255)
                                                             ttGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGc                                                                           <  3:120312/149‑1 (MQ=255)
                                                             ttGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGc                                                                           <  4:12457/149‑1 (MQ=255)
                                                             tggggggAGAAGCGCTGGGGGTACACGCATATCGCGGTTTGCAGGCGATCGTCACCCATGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGc                                                                           <  3:268120/144‑1 (MQ=255)
                                                               ggtggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGTGAAGCTGCAGATGTAGTGGTCGGcgt                                                                         >  4:122836/1‑149 (MQ=255)
                                                               ggtggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCgttcg                                                                         >  1:139449/1‑145 (MQ=255)
                                                                gtggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgtc                                                                        <  3:70589/149‑1 (MQ=255)
                                                                gtggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgtc                                                                        >  1:201849/1‑149 (MQ=255)
                                                                 tggAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGcgtcg                                                                       >  7:331703/1‑149 (MQ=255)
                                                                      aaGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGc                                                                  <  2:281514/149‑1 (MQ=255)
                                                                              gtggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCa                                                          >  3:317075/1‑149 (MQ=255)
                                                                               tggAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCAc                                                         <  2:201849/149‑1 (MQ=255)
                                                                                   acacGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACcgac                                                     >  3:112022/1‑149 (MQ=255)
                                                                                       gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                 >  8:203393/1‑149 (MQ=255)
                                                                                       gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGAAGATGTAGTGGTCGGCGTAGTGGGCGTTGGCCACCGacgaac                                                 >  8:171659/1‑149 (MQ=255)
                                                                                       gCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGCCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                 >  4:143554/1‑149 (MQ=255)
                                                                                            tCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAacgacga                                            >  4:44906/1‑149 (MQ=255)
                                                                                                ggTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACggg                                        <  5:335789/149‑1 (MQ=255)
                                                                                                         gcgcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGAGGATGGTCTTGAGCGAGAAGCTGCAGATGTAGTGTTCGGCGTCGTGGGCGTTGGCAACCGAGGAACGACGACGTGCACTGaa                                 >  6:358455/1‑147 (MQ=255)
                                                                                                          cgcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACcgac                                                     >  5:2752/1‑126 (MQ=255)
                                                                                                          cgcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACcgac                                                     <  6:2752/126‑1 (MQ=255)
                                                                                                           gcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCCGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGc                             <  7:203393/149‑1 (MQ=255)
                                                                                                           gcTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGc                             <  2:308745/149‑1 (MQ=255)
                                                                                                               gTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGTGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCtt                           <  3:122836/147‑1 (MQ=255)
                                                                                                               gTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTg                          <  4:317075/148‑1 (MQ=255)
                                                                                                                  aCCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATgg                      >  1:250350/1‑149 (MQ=255)
                                                                                                                  aCCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATg                       <  3:44906/148‑1 (MQ=255)
                                                                                                                   cccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGc                     <  8:343260/149‑1 (MQ=255)
                                                                                                                    ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGc                     >  5:361672/1‑148 (MQ=255)
                                                                                                                    ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGc                     >  1:337929/1‑148 (MQ=255)
                                                                                                                    ccAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGc                     >  1:68416/1‑148 (MQ=255)
                                                                                                                          cTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTcc              >  5:309464/1‑149 (MQ=255)
                                                                                                                             gATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGGACTGAACAGCTTGATGGCGCATTCCTgg           >  8:191339/1‑149 (MQ=255)
                                                                                                                                  gatgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCt             >  6:290916/4‑142 (MQ=255)
                                                                                                                                  gatgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCt             <  5:290916/139‑1 (MQ=255)
                                                                                                                                  aaCGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCt      >  3:73513/1‑149 (MQ=255)
                                                                                                                                   atgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCt      >  1:8710/3‑148 (MQ=255)
                                                                                                                                   atgccgcGCGATCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCt      >  1:131575/3‑148 (MQ=255)
                                                                                                                                    cgccgcGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCa    <  7:73946/149‑1 (MQ=255)
                                                                                                                                       cgcgAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGAAGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAgg  >  3:232798/1‑148 (MQ=255)
                                                                                                                                          gcgaTCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                 <  1:41040/96‑1 (MQ=255)
                                                                                                                                          gcgaTCTCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGacgaac                                                 >  2:41040/3‑98 (MQ=255)
                                                                                                                                                |                                                                                                                                          
GATCTCGCCGTTGTGGGCGAGGAAGCGGAACGGCTGCGCCAGCGGCCATTTCGGCAGGGTGTTGGTGGAGAAGCGCTGGTGGAACACGCAGATCGCGGTTTGCAGGCGCTCGTCACCCAGGTCTGGATAAAACGCCGCGAGATCGCGCGGCATCATCAGGCCTTTGTAGATGATGGTCTTGTGCGAGAAGCTGCAGATGTAGTGGTCGGCGTCGTGGGCGTTGGCCACCGACGAACGACGACGGGCACTGAACAGCTTGATGGCGAATTCCTGGTCGCTCAGG  >  NC_002947/5797168‑5797450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑