BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,824,714	T→A	5.9%	intergenic (+138/+36)	PP_5102 → / ← trmB	hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,824,714	0	T	A	5.9%	320.2 / 6.0	101	intergenic (+138/+36)	PP_5102/trmB	hypothetical protein/tRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand): ref base T (50/45); new base A (4/2); total (54/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 101 total aligned reads displayed.

CGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCG  >  NC_002947/5824568‑5824858

cgcgGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCa                                                                                                                                                  <  3:119859/149‑1 (MQ=255)

 gcgGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCac                                                                                                                                                 <  4:323738/149‑2 (MQ=255)

   ggCTGAGCGAATGACTCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAggg                                                                                                                                               <  2:303374/149‑1 (MQ=255)

    gCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑c                                                                                                                                             >  2:158870/1‑149 (MQ=255)

       gAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGt                                                                                                                                          <  4:97443/149‑1 (MQ=255)

           gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑a                                                                                                                                     >  8:173432/1‑149 (MQ=255)

           gAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGG‑CGGttttt                                                                                                                                      >  1:30007/1‑148 (MQ=255)

            aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCTGGCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑a                                                                                                                                     >  1:81556/1‑149 (MQ=255)

            aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑Ac                                                                                                                                    <  3:258463/149‑1 (MQ=255)

            aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑Ac                                                                                                                                    <  2:240962/149‑1 (MQ=255)

            aaTGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGG‑CGGtttttt                                                                                                                                     <  2:30007/149‑3 (MQ=255)

                aCCCCTTGTGGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGttttttctt                                                                                                                                  <  8:42779/148‑6 (MQ=255)

                       gTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGcc                                                                                                                          <  7:173432/148‑1 (MQ=255)

                       gTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGc                                                                                                                           <  2:81556/147‑1 (MQ=255)

                           gAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGcc                                                                                                                          >  7:127000/1‑144 (MQ=255)

                           gAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGcc                                                                                                                          <  8:127000/144‑1 (MQ=255)

                           gAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTAt                                                                                                                      >  1:194860/1‑148 (MQ=255)

                            aGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTAt                                                                                                                      >  2:248607/1‑147 (MQ=255)

                            aGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTAt                                                                                                                      >  8:351724/1‑147 (MQ=255)

                                gATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCTGCCg                                                                                                                >  7:80964/1‑149 (MQ=255)

                                gATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCg                                                                                                                <  2:194860/149‑1 (MQ=255)

                                     aaCCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAACGGCCGTATCAGCCGCTCaa                                                                                                           >  4:173138/1‑149 (MQ=255)

                                     aaCCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCaa                                                                                                           <  1:158870/149‑1 (MQ=255)

                                     aaCCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCa                                                                                                            >  7:40204/1‑148 (MQ=255)

                                      aCCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAAt                                                                                                          >  3:272994/1‑149 (MQ=255)

                                         gcgAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCACGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCa                                                                                                       >  1:110658/1‑149 (MQ=255)

                                           gAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCAcc                                                                                                     >  7:307307/1‑149 (MQ=255)

                                              aGGGCCGGTTTT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTtc                                                                                                  <  1:316986/149‑1 (MQ=255)

                                                ggccggccCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTtctc                                                                                                <  6:363127/149‑1 (MQ=255)

                                                   cggccCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGaa                                                                                             <  5:368701/149‑1 (MQ=255)

                                                       ccT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACtt                                                                                          >  2:85126/1‑148 (MQ=255)

                                                           gctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAg                                                                                      >  2:89309/1‑149 (MQ=255)

                                                            ctgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGGCCGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCaa                                                                                       <  1:98003/148‑1 (MQ=255)

                                                             tgGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTc                                                                                    >  3:285219/1‑149 (MQ=255)

                                                               gCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTcc                                                                                   <  5:209372/148‑1 (MQ=255)

                                                                 cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGt                                                                                     >  1:95507/1‑144 (MQ=255)

                                                                 cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGt                                                                                     <  2:95507/144‑1 (MQ=255)

                                                                 cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCca                                                                                 >  8:114410/1‑148 (MQ=255)

                                                                 cAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCca                                                                                 >  5:171841/1‑148 (MQ=255)

                                                                  aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGG‑CGGttttt                                                                                                                                      <  6:112096/95‑2 (MQ=18)

                                                                  aaTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTGAAAAGGG‑CGGttttt                                                                                                                                      >  5:112096/1‑94 (MQ=18)

                                                                   aTCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCacac                                                                              >  4:205177/1‑149 (MQ=255)

                                                                    tctcCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAcc                                                                             >  5:351604/1‑149 (MQ=255)

                                                                      tcCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAcccc                                                                           >  4:259292/1‑149 (MQ=255)

                                                                      tcCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAcccc                                                                           >  5:345268/1‑149 (MQ=255)

                                                                        cACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCATTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGACGCTCAATCCACCATCTCGAACTTCAAGTCCCACCCCCCGt                                                                         >  6:364946/1‑149 (MQ=255)

                                                                         aCCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTg                                                                        >  8:211748/1‑149 (MQ=255)

                                                                           ccGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGCGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGcc                                                                      >  8:199447/1‑149 (MQ=255)

                                                                                cTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGc                                                                 >  7:114710/1‑149 (MQ=255)

                                                                                   cACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCTATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCg                                                               <  1:265069/148‑1 (MQ=255)

                                                                                    aCCGGGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGc                                                              <  8:3295/148‑1 (MQ=255)

                                                                                      cGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCa                                                           <  7:114410/149‑1 (MQ=255)

                                                                                        tGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAAACGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCAcc                                                         >  6:290847/1‑149 (MQ=255)

                                                                                          ggCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑AGCGAAAAGGCCGTATCAGACGCTCAATCCACAATCTCGAACTTCAAGTCACACACCCCGTGCACCAGCCGCGCAAcgg                                                       >  4:12124/1‑148 (MQ=38)

                                                                                                gcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGtt                                                 <  5:364946/149‑1 (MQ=255)

                                                                                                gcTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGtt                                                 >  8:57822/1‑149 (MQ=255)

                                                                                                 cTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTc                                                <  5:290847/149‑1 (MQ=255)

                                                                                                 cTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTc                                                <  3:259292/149‑1 (MQ=255)

                                                                                                    ccGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGaa                                             <  4:285219/149‑1 (MQ=255)

                                                                                                    ccGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGaa                                             >  2:245132/1‑149 (MQ=255)

                                                                                                     cGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAAc                                            >  8:164816/1‑149 (MQ=255)

                                                                                                     cGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGACCCAGCCGCTCACCGCGGCGTTCGAAc                                            >  4:264068/1‑149 (MQ=255)

                                                                                                       tATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACtt                                          >  8:248093/1‑149 (MQ=255)

                                                                                                         ttCGCGTGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTCTCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                         <  6:138048/148‑1 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                        <  6:345268/149‑1 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                        >  7:159035/1‑149 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTgg                                        >  7:163924/1‑149 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                         <  3:176302/148‑1 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                         <  2:110658/148‑1 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                         <  4:272994/148‑1 (MQ=255)

                                                                                                         ttCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTg                                         <  7:164816/148‑1 (MQ=255)

                                                                                                            gcgGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTTAACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGt                                       >  1:97409/1‑148 (MQ=255)

                                                                                                              gggTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATc                                   <  7:351724/149‑1 (MQ=255)

                                                                                                                gTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCgg                                 <  8:40204/149‑1 (MQ=255)

                                                                                                                   aaCCCGCTCCTACAAAGAAAAAACCTCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgc                               <  8:307307/148‑1 (MQ=255)

                                                                                                                    aCCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGgcgc                             <  6:171841/149‑1 (MQ=255)

                                                                                                                     cccGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGct                            >  1:241539/1‑149 (MQ=255)

                                                                                                                      ccGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGctc                           >  6:24006/1‑149 (MQ=255)

                                                                                                                      ccGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGctc                           >  5:130570/1‑149 (MQ=255)

                                                                                                                          tCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGACCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCg                       >  5:289481/1‑149 (MQ=255)

                                                                                                                            cTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCg                       <  1:245132/147‑1 (MQ=255)

                                                                                                                            cTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCg                       <  8:114710/147‑1 (MQ=255)

                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAACGGCCGTATCAGCCGCTCACTCCACGTTCTCGAACTTCAAGTCCCCCACCGCGTGGCCCAGCCGCTCACCGCGGCGTTCGAACAGGGTGCGCGGGCGCTCATCGGGgcgc                 >  4:280297/1‑149 (MQ=255)

                                                                                                                                aaaGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCCCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 >  8:218525/1‑149 (MQ=255)

                                                                                                                                 aaGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGcgcg                >  5:60953/1‑149 (MQ=255)

                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg               <  3:205177/149‑1 (MQ=255)

                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg               >  5:363070/1‑149 (MQ=255)

                                                                                                                                  aGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCgg               >  6:54836/1‑149 (MQ=255)

                                                                                                                                   ggtaaaaCCGCCCTGAAAAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTATTCGGCGCGCGGc              >  6:308002/4‑149 (MQ=255)

                                                                                                                                   gAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGc              <  7:49192/149‑1 (MQ=255)

                                                                                                                                    aaaaaaCCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAACCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGca             >  4:336692/1‑149 (MQ=255)

                                                                                                                                      aaaaCCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGcaca           <  3:12124/149‑1 (MQ=255)

                                                                                                                                      aaaaCCGCCCTGAAAAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGcaca           >  1:268428/1‑149 (MQ=255)

                                                                                                                                         aCCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 <  3:54322/140‑1 (MQ=255)

                                                                                                                                         aCCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAg        <  6:351604/149‑1 (MQ=255)

                                                                                                                                         aCCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACAACCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGgcgc                 >  4:54322/1‑140 (MQ=255)

                                                                                                                                           cGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt      <  7:97033/149‑1 (MQ=255)

                                                                                                                                           cGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGt      <  7:199447/149‑1 (MQ=255)

                                                                                                                                            gCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTg     >  2:13330/1‑149 (MQ=255)

                                                                                                                                               cTTTTCACGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCg  <  3:148181/149‑1 (MQ=255)

CGCGGCTGAGCGAATGACCCCTTGTAGGAGCGGATTCAACCGCGAAAGGGCCGGCCCT‑GCTGGCCAATCTCCACCCGCCCTTCACCGTGGGCGCCGCTACCGTATTCGCGGGTAAACCCGCTCCTACAAAGAAAAAACCGCCCTTTTCAGGG‑CGGTTTT‑ACCGAAAAGGCCGTATCAGCCGCTCAATCCACCTTCTCGAACTTCAAGTCCCACACCCCGTGCCCCAGCCGCTCACCGCGGCGTTCGAACTTGGTGATCGGGCGCTCTTCGGGGCGCGGCACATAGGTGCCG  >  NC_002947/5824568‑5824858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`