Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,897,778 C→G 8.9% intergenic (‑178/+56) PP_5172 ← / ← PP_5173 hypothetical protein/RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,897,7780CG 8.9% 138.9 / 5.1 55intergenic (‑178/+56)PP_5172/PP_5173hypothetical protein/RND family transporter
Reads supporting (aligned to +/- strand):  ref base C (22/27);  new base G (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

CAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATC  >  NC_002947/5897631‑5897924
                                                                                                                                                   |                                                                                                                                                  
cAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTc                                                                                                                                                    >  4:288308/1‑148 (MQ=255)
  gagGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGAt                                                                                                                                                 <  2:39649/149‑1 (MQ=255)
   agGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATg                                                                                                                                                <  6:197474/149‑1 (MQ=255)
    ggTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGc                                                                                                                                               <  8:348515/149‑1 (MQ=255)
          aaGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATc                                                                                                                                         >  8:174461/1‑149 (MQ=255)
             gggTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCAc                                                                                                                                             >  4:121835/1‑142 (MQ=255)
             gggTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCAc                                                                                                                                             <  3:121835/142‑1 (MQ=255)
               gTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCAtt                                                                                                                                    >  4:22641/1‑149 (MQ=255)
                    gcgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgcg                                                                                                                                <  5:275025/148‑1 (MQ=255)
                     cgcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                               <  6:81548/148‑1 (MQ=25)
                     cgcCTGGCTTGACGGCAATTGTAGGGAGGCGGGATTATTCTTAAAAATACTGGTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCgg                                                                                                                               <  8:63388/148‑1 (MQ=21)
                      gcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCggg                                                                                                                              >  2:35764/1‑148 (MQ=255)
                      gcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCggg                                                                                                                              >  7:164291/1‑148 (MQ=255)
                      gcCTGGCTTGAGGGCAATAGTAGTGAGGCGGGAGTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGt                                                                                                                             <  7:260862/149‑1 (MQ=25)
                             ttGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTATGGTAGGAGCGGGTTTACCAGCGAATACGGTGGTAGATGCACCATCGCAATCGCGGGTAAACCCg                                                                                                                      >  3:75696/1‑149 (MQ=255)
                               gAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGc                                                                                                                     <  3:288308/148‑1 (MQ=255)
                                aGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCt                                                                                                                    <  6:193812/148‑1 (MQ=255)
                                aGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTCGATGCACCATAGCATTCGCGGGTAAACCCGCTc                                                                                                                   >  5:7256/1‑149 (MQ=255)
                                   gCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTa                                                                                                                >  4:121815/1‑149 (MQ=255)
                                       tagtagTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAg                                                                                                            <  1:35764/149‑1 (MQ=255)
                                          ttgtGAGGCGGTTTTTTTCTTAAAAATCCTTTTTAATATTTTTTATAAACCCATTAAAGACAACACGCCTCTGTTCGGCGCGGTTTTACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGTTAAACCCGCTCCTACCAggg                                                                                                          <  2:157430/146‑1 (MQ=255)
                                                  cGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATgg                                                                                                 >  2:256900/1‑149 (MQ=255)
                                                        aattCTTAAAAATACTGTTTAATAATTTTTAAATAACCATTAAAGACAACACGCCTCTGGTAGGAGCGGTTTTACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCtt                                                                                            <  6:7256/147‑1 (MQ=38)
                                                             ttAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCg                                                                                      >  8:257167/1‑149 (MQ=255)
                                                               aaaaaTACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCgt                                                                                     <  7:220846/148‑1 (MQ=255)
                                                               aaaaaTACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCgtg                                                                                    >  8:267831/1‑149 (MQ=255)
                                                                aaaaTACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCgtg                                                                                    <  1:84358/148‑1 (MQ=255)
                                                                  aaTACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATAAGGTGGTCGAAGCCCCATCGCATTCGCGGGGAAAACAGCTCCTACCACGGGCCATAAGGGCCGTGAGGCCTGtt                                                                                  >  2:297782/1‑148 (MQ=255)
                                                                         tttAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGc                                                                          >  7:249931/1‑149 (MQ=255)
                                                                         tttAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTTGTCGATTCACCATCGCATTACCGGGTAAACCCACTACTACCACGGGCCATATTTGCCTTAACTCGTTTTTATGCgg                                                                           >  8:162661/1‑148 (MQ=255)
                                                                          ttAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGc                                                                          <  3:121815/148‑1 (MQ=255)
                                                                             aGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCTAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTcgc                                                                      <  8:164291/149‑1 (MQ=255)
                                                                                    ttATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCt                                                                <  7:174461/148‑1 (MQ=255)
                                                                                          aaCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTAACCGCGAATACGGTGGTCGCTGCACCATCGCATTAGCGGGTTAAACCGCTCCTACCACGGGCCATATGGGCCGTCAGGCGTGTTTGTGCGGCTCGCGGATCtttttc                                                           >  1:345133/1‑143 (MQ=255)
                                                                                           aCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAg                                                        <  3:22641/149‑1 (MQ=255)
                                                                                           aCCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAg                                                        >  7:155009/1‑149 (MQ=255)
                                                                                            ccATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAgg                                                       >  3:295101/1‑149 (MQ=255)
                                                                                                 tAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGTGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCAc                                                   <  4:107966/148‑1 (MQ=255)
                                                                                                     agaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACa                                              <  7:257167/149‑1 (MQ=255)
                                                                                                      gaACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCGCCTACCAGGGGCCATATGGGCCTTGAGGCGTGTTTCTGCGGCTGGCGGGTCTTGTACCAGGCCCCGTACAg                                             >  3:161771/1‑149 (MQ=38)
                                                                                                       aaCACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGc                                                                                               <  8:294529/98‑1 (MQ=255)
                                                                                                       aaCACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGc                                                                                               >  7:294529/1‑98 (MQ=255)
                                                                                                          acGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGcc                                         >  5:190533/1‑149 (MQ=255)
                                                                                                          acGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGcc                                         <  6:190533/149‑1 (MQ=255)
                                                                                                              ctctGGTAGGAGCGGGTTTTACCCCGAATACGGTGGTGGATGGACCATAGCATTAGCGGGTAAAACCGCTCCTAACAGGGGGCATATTGGGCGTGAGGGGTGTTTATGGGGCGGGCGGGTGTTGTTCCACGGCACCTAAAGCGcccg                                       >  8:191589/1‑145 (MQ=255)
                                                                                                              ctctGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCa                                     <  7:169409/149‑1 (MQ=255)
                                                                                                                 tGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAg                                    <  7:191589/147‑1 (MQ=255)
                                                                                                                   gttgtcgCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGTTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCTGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGaa                                 <  4:75696/142‑1 (MQ=255)
                                                                                                                   gTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGaa                                 <  1:256900/148‑1 (MQ=255)
                                                                                                                         gCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc                                                                                                                                 <  2:327578/46‑1 (MQ=255)
                                                                                                                         gCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTcgc                                                                                                                                 >  1:327578/1‑46 (MQ=255)
                                                                                                                           gggTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGt                        <  1:297782/149‑1 (MQ=255)
                                                                                                                                   ccGCGAATGCGATGGTGCATCGACCACCGTATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAgg                >  2:339192/1‑149 (MQ=25)
                                                                                                                                     gcgAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTAc                                               >  4:221985/1‑116 (MQ=255)
                                                                                                                                          tACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGGACAGCGCCGGCAGGAACAGCAGCGTCCGCACGGTCGCAAc         >  6:259571/1‑149 (MQ=255)
                                                                                                                                                 gtCGATGCCCAATCGCATTCGCGGTTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATc  <  2:359539/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGAGGTTTGAAGGGGTTTTGCGCCTGGCTTGAGGGCAATAGTAGTGAGGCGGGATTATTCTTAAAAATACTGTTTAAGAATTTTTATATAACCATTTAAGAGAACACGCCTCTGGTAGGAGCGGGTTTACCCGCGAATACGGTGGTCGATGCACCATCGCATTCGCGGGTAAACCCGCTCCTACCAGGGGCCATATGGGCCTTCAGGCGTGTTTCTGCGGCTCGCGGATCTTGTACCAGGCCACGTACAGCGCCGGCAGGAACAGCAGCGTCAGCAAGGTCGCCACGATGATC  >  NC_002947/5897631‑5897924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑