BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,940,748	T→A	5.3%	L196Q (CTG→CAG)	yhhJ →	translation‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,940,748	0	T	A	5.3%	282.1 / 4.1	94	L196Q (CTG→CAG)	yhhJ	translation‑like protein
Reads supporting (aligned to +/- strand): ref base T (51/38); new base A (3/2); total (54/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

Only 100 of 102 total aligned reads displayed.

TACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAGTCGTCAAAGGTGC  >  NC_002947/5940600‑5940895

tACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAATGCGCTGTACAACCCCAACCTGGAGCGGGCCTGGATTCTCGCCCAGCTCCAGATCGTCAACAACATTACCATCCTGCCGATCATCCTCACTGGCACCCCgct                                                                                                                                                      >  5:2295/1‑149 (MQ=255)

  cagccagcGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATTCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCgctgc                                                                                                                                                    >  6:6869/1‑149 (MQ=255)

   agccagcGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCgctgct                                                                                                                                                   >  5:229150/1‑149 (MQ=255)

     ccagcGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCtgctgc                                                                                                                                                 <  4:233079/149‑1 (MQ=255)

     ccagcGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCtgctgc                                                                                                                                                 <  7:130121/149‑1 (MQ=255)

      cagcGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTgcg                                                                                                                                                >  4:178213/1‑149 (MQ=255)

             gTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCAGCAGcgcggtgc                                                                                                                                          <  2:5286/148‑5 (MQ=255)

                      aaaGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGc                                                                                                                                >  6:46592/1‑149 (MQ=255)

                       aaGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCa                                                                                                                               >  4:295091/1‑149 (MQ=255)

                        aGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCAGCAGcgcggtgcca                                                                                                                                        >  1:375183/1‑133 (MQ=255)

                        aGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCAGCAGcgcggtgcca                                                                                                                                        <  2:375183/139‑7 (MQ=255)

                                   gATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGaccacc                                                                                                                   >  2:342214/1‑149 (MQ=255)

                                    aTCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACct                                                                                                                  >  8:248868/1‑149 (MQ=255)

                                     tCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACctg                                                                                                                 >  4:281608/1‑149 (MQ=255)

                                        aCCCCAAGGCGCTGTTCTACCCCAACCTGGAGGGTGGCTGTTTTCTTGCCGTGATCCAGATCGTCAACAACTTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCTTGAGCATGGCACCCTCGACCACctg‑ctg                                                                                                             <  6:2295/149‑1 (MQ=255)

                                        aCCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACctg‑ctg                                                                                                             >  7:42304/1‑149 (MQ=255)

                                        aCCCCAAGGCGCTGTTAAACCCCAACCTGGAGGGGGGCTGGTTTCAGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACctg‑ctg                                                                                                             >  1:295779/1‑149 (MQ=255)

                                         ccccAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTgg                                                                                                            >  7:253491/1‑149 (MQ=255)

                                         ccccAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTgg                                                                                                            >  7:320827/1‑149 (MQ=255)

                                          cccAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGt                                                                                                           >  7:129220/1‑149 (MQ=255)

                                          cccAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGt                                                                                                           >  5:17475/1‑149 (MQ=255)

                                          cccAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGt                                                                                                           <  3:295091/149‑1 (MQ=255)

                                            cAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGtgc                                                                                                         >  5:168808/1‑149 (MQ=255)

                                               ggCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGtgctgc                                                                                                      >  5:301852/1‑149 (MQ=255)

                                                gcgcTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGcc                                                                                                     >  3:325277/1‑149 (MQ=255)

                                                    tGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCt                                                                                                  >  8:130134/1‑148 (MQ=255)

                                                        cAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACcg                                                                                             >  4:328408/1‑149 (MQ=255)

                                                         aaCCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACcgc                                                                                            <  7:130134/149‑1 (MQ=255)

                                                           ccccAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCgcgc                                                                                          >  5:200791/1‑149 (MQ=255)

                                                            cccAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCt                                                                                         >  7:217523/1‑149 (MQ=255)

                                                               aaCCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGCGCATGGCACCCTAGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTg                                                                                        >  4:347145/1‑147 (MQ=255)

                                                                   tGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGAt                                                                                   >  2:328260/1‑148 (MQ=255)

                                                                     gAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATc                                                                                  <  5:17549/147‑1 (MQ=255)

                                                                     gAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCa                                                                                 <  6:168808/148‑1 (MQ=255)

                                                                      aGGGGTGCTGGTTTCTTGCCGTGATCCAGATCGTCAACAACATTTCCATCATGGCGATCATCATCACTGGCACCGCGCTGCTGCGCGAGCGTTAGCATGGCACCCTCGACCACCTG‑CTGGTGATGCAGATGACCTCGCTGGAGATCATg                                                                               <  6:84308/149‑1 (MQ=255)

                                                                        gggggCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTGCCTGGTGCTGCCGCTGACCGCGCTGGAGATCATGc                                                                              <  1:50679/149‑1 (MQ=255)

                                                                          gggCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTgg                                                                           <  8:217523/149‑1 (MQ=255)

                                                                          gggCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCCGCCGCTGACCGCGCTGGAGATCATGCTgg                                                                           <  1:342214/149‑1 (MQ=255)

                                                                                gTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGcc                                                                         >  6:37819/1‑145 (MQ=255)

                                                                                   tCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATc                                                                   <  6:200791/148‑1 (MQ=255)

                                                                                    cTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCgg                                                                 >  5:319697/1‑149 (MQ=255)

                                                                                      ggCCGTGATCCAGATCGTCAACATCATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCt                                                                             <  5:301065/135‑1 (MQ=255)

                                                                                      ggCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCt                                                                             >  6:301065/1‑135 (MQ=255)

                                                                                      ggCCGTGATCCAGATCGGCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGgca                                                               <  2:312973/149‑1 (MQ=255)

                                                                                         cGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCAcc                                                                                                                             <  2:88457/85‑1 (MQ=255)

                                                                                         cGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCAcc                                                                                                                             >  1:88457/1‑85 (MQ=255)

                                                                                          gTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCaa                                                           >  5:249050/1‑149 (MQ=255)

                                                                                              tCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAAcgcg                                                       >  7:201848/1‑149 (MQ=255)

                                                                                                      gTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggt                                               <  5:364763/149‑1 (MQ=255)

                                                                                                       tCAACAACATTACCATCCTGGCGATCATCGTCAGTGGCACCGCGCTGGTGCGCGAGCGTGAGCATGGCACCCTCGACCACGTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCTTGGTGGGCAAGATCGGCAGCATCGCGCtggtggtg                                              <  2:295779/149‑1 (MQ=255)

                                                                                                       tCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtg                                              <  6:319697/149‑1 (MQ=255)

                                                                                                        caacaaCATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtgg                                             <  7:248868/149‑1 (MQ=255)

                                                                                                         aacaacATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtgg                                             <  7:237798/148‑1 (MQ=255)

                                                                                                         aacaacATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtgg                                             >  8:237798/1‑148 (MQ=255)

                                                                                                         aacaacATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTggtggtggt                                            >  4:65575/1‑149 (MQ=255)

                                                                                                         aacaacATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTggtggtggt                                            >  8:328436/1‑149 (MQ=255)

                                                                                                         aacaacATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCAAGCTGGCCAAGATCGGCAGCAACGCGCTggtggtggt                                            >  7:221697/1‑149 (MQ=255)

                                                                                                           caacaTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGa                                          <  8:129220/149‑1 (MQ=255)

                                                                                                            aacaTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGAt                                         <  8:221697/149‑1 (MQ=255)

                                                                                                             acaTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATc                                        >  5:277716/1‑149 (MQ=255)

                                                                                                              caTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCt                                       >  8:215410/1‑149 (MQ=255)

                                                                                                              caTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCt                                       <  3:281608/149‑1 (MQ=255)

                                                                                                               aTTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCt                                       >  4:336014/1‑148 (MQ=255)

                                                                                                                ttACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTg                                      >  7:77898/1‑148 (MQ=255)

                                                                                                                   ccATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCAcc                                  >  7:190826/1‑149 (MQ=255)

                                                                                                                   ccATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCAc                                   <  6:17475/148‑1 (MQ=255)

                                                                                                                    cATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCAGCt                                 <  8:320827/149‑1 (MQ=255)

                                                                                                                     aTCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTg                                <  5:37819/149‑1 (MQ=255)

                                                                                                                     aTCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGAGCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTg                                <  6:301852/149‑1 (MQ=255)

                                                                                                                         tGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATg                                                                               >  4:88958/1‑98 (MQ=255)

                                                                                                                         tGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATg                                                                               <  3:88958/98‑1 (MQ=255)

                                                                                                                             gATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGt                        >  3:97270/1‑149 (MQ=255)

                                                                                                                             gATCATCCTCACTGGCACAGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGAGGTGATCTGCACCTGGATTTCGt                        >  5:159075/1‑149 (MQ=255)

                                                                                                                              atcatcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGtt                       <  8:42304/149‑1 (MQ=255)

                                                                                                                               tcatcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtg                      <  3:328408/149‑1 (MQ=255)

                                                                                                                                catcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtgg                     >  3:107813/1‑149 (MQ=255)

                                                                                                                                 atcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCTCCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACATGGATTTCGTtggt                    >  4:14358/1‑149 (MQ=255)

                                                                                                                                 atcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtggt                    <  3:178213/149‑1 (MQ=255)

                                                                                                                                 atcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtggt                    <  1:328260/149‑1 (MQ=255)

                                                                                                                                 atcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtgg                     <  4:97270/148‑1 (MQ=255)

                                                                                                                                 atcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtgg                     <  7:343777/148‑1 (MQ=255)

                                                                                                                                  tcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTTATCTGCACCTGGATTTCGt                        <  4:74611/144‑1 (MQ=255)

                                                                                                                                  tcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTTATCTGCACCTGGATTTCGTtggtg                   <  5:28523/149‑1 (MQ=255)

                                                                                                                                  tcCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtggtg                   <  5:6869/149‑1 (MQ=255)

                                                                                                                                  tcCTCACTGGCACAGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGt                        >  3:74611/1‑144 (MQ=255)

                                                                                                                                   ccTCACTGGCACGGCGCTGCTGCGCGAGCTTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACGGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtggtg                   <  6:249050/148‑1 (MQ=255)

                                                                                                                                   ccTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGTAGATCATGCTGAACAAGATCTGAAGCAACGCGCTGGTCGTGGTGATCTGCAACTGGATTTCGTtggtgt                  >  6:283282/1‑148 (MQ=255)

                                                                                                                                   ccTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtggtgg                  <  4:107813/149‑1 (MQ=255)

                                                                                                                                    cTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTggtggt                 >  3:3342/1‑149 (MQ=255)

                                                                                                                                         tGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAgtcg            >  7:148994/1‑149 (MQ=255)

                                                                                                                                         cacgctcgcgCAGCAGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAgtcg            >  5:232270/7‑149 (MQ=255)

                                                                                                                                           gCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtg                                              >  4:102249/1‑113 (MQ=255)

                                                                                                                                           gCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCtggtggtg                                              <  3:102249/113‑1 (MQ=255)

                                                                                                                                            gctcgcgCAGCAGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAgtcgtc          >  6:226500/4‑148 (MQ=255)

                                                                                                                                            cACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGCTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGCTGGAGATCTGCACCTGGATTTCGTTGGTGGTAgtcgtc          >  2:344303/1‑148 (MQ=255)

                                                                                                                                                gcgcTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAGTCGTCAAAgg     >  1:23947/1‑149 (MQ=255)

                                                                                                                                                 cgcTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAGTCGTCAAAGGt    >  3:258369/1‑149 (MQ=255)

                                                                                                                                                    tgctgcGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtgg                     >  2:373737/1‑129 (MQ=255)

                                                                                                                                                    tgctgcGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTtgg                     <  1:373737/129‑1 (MQ=255)

                                                                                                                                                    tgctgaGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCTCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGAAGCAACGCGCTGGTGGAGGTGATCTGCACCTGGATTTCGTTGTTGGTAGTCGTCAAAGGAGc  >  2:296905/1‑148 (MQ=255)

TACAGCCAGCGTGGTAACGAGCAAAGCCCGGTGCTGATCAACCCCAAGGCGCTGTTCAACCCCAACCTGGAGGGGGGCTGGTTTCTGGCCGTGATCCAGATCGTCAACAACATTACCATCCTGGCGATCATCCTCACTGGCACCGCGCTGCTGCGCGAGCGTGAGCATGGCACCCTCGACCACCTG‑CTGGTGCTGCCGCTGACCGCGCTGGAGATCATGCTGGCCAAGATCGGCAGCAACGCGCTGGTGGTGGTGATCTGCACCTGGATTTCGTTGGTGGTAGTCGTCAAAGGTGC  >  NC_002947/5940600‑5940895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`