Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,015,701 G→C 6.7% H22D (CAT→GAT)  PP_5266 ← acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,015,7010GC 6.7% 243.0 / 6.0 75H22D (CAT→GAT) PP_5266acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base C (3/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

CCGCTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAACCC  >  NC_002947/6015555‑6015848
                                                                                                                                                     |                                                                                                                                                   
ccgcTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatgga                                                                                                                                                   <  6:192505/149‑1 (MQ=255)
      catggcATAGCCCACTGGCGGGGGGGCGTTGGTAATTGCTGGAAT‑TGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatggatatgg                                                                                                                                              <  1:311770/144‑1 (MQ=255)
      cAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATggg                                                                                                                                             >  7:245625/1‑149 (MQ=255)
      cAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATggg                                                                                                                                             >  7:291943/1‑149 (MQ=255)
      cAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAATGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATggg                                                                                                                                             <  4:250888/149‑1 (MQ=255)
       aGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCAGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGc                                                                                                                                            <  1:81019/149‑1 (MQ=255)
       aGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGAGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGc                                                                                                                                            >  1:56432/1‑149 (MQ=255)
        ggCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCt                                                                                                                                           >  5:123074/1‑149 (MQ=255)
           cATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTgg                                                                                                                                         >  4:346221/1‑148 (MQ=255)
           cATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTgg                                                                                                                                         >  6:133402/1‑148 (MQ=255)
               gCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCCTGTTCGGGCAGCTCGCGTACGTGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGc                                                                                                                                     >  1:92549/1‑149 (MQ=255)
                cccAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatggatatgg                                                                                                                                              <  8:335948/138‑1 (MQ=255)
                cccAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCg                                                                                                                                   >  5:339445/1‑149 (MQ=255)
                cccAGGGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCatatggatatgg                                                                                                                                              >  7:335948/1‑138 (MQ=255)
                 ccAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTATGAGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGCCGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATCGATATGGGCTGGCAGCCg                                                                                                                                   >  1:49708/1‑149 (MQ=255)
                 ccAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCgg                                                                                                                                  <  1:90850/149‑1 (MQ=255)
                  cAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCgg                                                                                                                                  >  6:336011/1‑148 (MQ=255)
                  cAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCgg                                                                                                                                  <  5:336011/148‑1 (MQ=255)
                       gcgggcggCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGAc                                                                                                                               >  3:113071/1‑146 (MQ=255)
                       gcgggcggCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACaa                                                                                                                             <  5:182898/148‑1 (MQ=255)
                         gggcggCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAc                                                                                                                          <  8:199913/149‑1 (MQ=255)
                          ggcggcCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTACGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAcc                                                                                                                         <  5:325484/149‑1 (MQ=255)
                              gcCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAc                                                                                                                          <  8:119959/144‑1 (MQ=255)
                              gcCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGAc                                                                                                                          >  7:119959/1‑144 (MQ=255)
                              gcCGAGGGAAAGTGC‑GGTAT‑AGATGGGCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGGTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCt                                                                                                                     <  4:31834/149‑1 (MQ=255)
                                  aGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATCCATATGGGcctgc                                                                                                                                        >  8:147628/1‑122 (MQ=255)
                                   gggAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCGAGGCCCAAAGGCAGGCCCAGATGGATTTGGGCTGGCAGCGGGGACAAGACCTGCTCGa                                                                                                                  >  2:302473/1‑147 (MQ=255)
                                    ggAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCAAc                                                                                                                 >  1:133804/1‑147 (MQ=255)
                                    ggAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATCCATATGGGcctgc                                                                                                                                        >  1:162642/1‑120 (MQ=255)
                                    ggAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATCCATATGGGcctgc                                                                                                                                        <  2:162642/124‑5 (MQ=255)
                                     gAAAGTGC‑GGTAT‑AGATCGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGc                                                                                                              <  4:273130/149‑1 (MQ=255)
                                          tGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCt                                                                                                                        <  1:80349/134‑1 (MQ=255)
                                          tGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCt                                                                                                                        >  2:80349/1‑134 (MQ=255)
                                           gC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCTGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGTCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGt                                                                                                         <  8:245360/148‑1 (MQ=255)
                                            c‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTc                                                                                                        >  6:36847/1‑148 (MQ=255)
                                               gTAT‑AGATGGGCAGGCGCC‑GTTCGGGCAGCGCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGAt                                                                                                     <  6:269084/149‑1 (MQ=255)
                                                ttt‑aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                    <  7:190046/147‑1 (MQ=255)
                                                tat‑aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                    <  8:245625/149‑1 (MQ=255)
                                                tat‑aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                    <  2:133804/149‑1 (MQ=255)
                                                 at‑aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATg                                                                                                    <  8:291943/148‑1 (MQ=255)
                                                  t‑aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGa                                                                                                  <  7:2894/149‑1 (MQ=255)
                                                    aGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAg                                                                                                 >  7:353045/1‑149 (MQ=255)
                                                     gATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGc                                                                                                <  7:9575/149‑1 (MQ=255)
                                                         gTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGTGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCagag                                                                                            <  2:92549/149‑1 (MQ=255)
                                                           cAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGt                                                                                          >  4:235969/1‑149 (MQ=255)
                                                             ggCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTT‑GGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCa                                                                                       >  5:149226/1‑149 (MQ=255)
                                                              gcgcC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCgg                                                                                                                                  <  4:303738/106‑1 (MQ=255)
                                                               cgcC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCAt                                                                                      <  6:339445/149‑1 (MQ=255)
                                                                gcC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGACGTGCATg                                                                                     >  7:336893/1‑149 (MQ=255)
                                                                          gCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGcctcct                                                                            <  8:122751/149‑1 (MQ=255)
                                                                                      cgcgGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGt                                                                 <  6:123074/148‑1 (MQ=255)
                                                                                       gcgGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCGGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTgg                                                               <  8:119104/149‑1 (MQ=255)
                                                                                         gggCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAACCTGGTCGATGGAGCa                                                                                               >  5:287762/1‑115 (MQ=255)
                                                                                         gggCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAACCTGGTCGATGGAGCa                                                                                               <  6:287762/115‑1 (MQ=255)
                                                                                                      gTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGa                                                 >  5:166392/1‑148 (MQ=255)
                                                                                                       ttGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACa                                               <  3:6676/149‑1 (MQ=255)
                                                                                                         tcccacggCGTTGGGGTTCCCCAGGCCCAAAGGCTCGCCCATATGGAAAGGGGCTGGCAGCCGGGACAAGCCCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                              <  6:21533/142‑1 (MQ=255)
                                                                                                         gACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                              <  8:353045/148‑1 (MQ=255)
                                                                                                         gACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                              <  3:235969/148‑1 (MQ=255)
                                                                                                          aCGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACTTGa                                            <  5:133402/149‑1 (MQ=255)
                                                                                                          aCGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGa                                            <  6:166392/149‑1 (MQ=255)
                                                                                                          aCGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATCGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGa                                            <  2:49708/149‑1 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                              <  5:331803/145‑1 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACAt                                              >  6:331803/1‑145 (MQ=255)
                                                                                                            gAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGc                                          >  1:422/1‑149 (MQ=255)
                                                                                                                  gTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGc                                    >  6:323770/1‑149 (MQ=255)
                                                                                                                      ggTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCggg                                 <  3:346221/148‑1 (MQ=255)
                                                                                                                             ccAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGc                                          >  2:349723/1‑132 (MQ=255)
                                                                                                                             ccAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGc                                          <  1:349723/132‑1 (MQ=255)
                                                                                                                             ccAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGc                         >  1:196420/1‑149 (MQ=255)
                                                                                                                               aGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGAc                                                                                                                 >  3:160874/1‑59 (MQ=255)
                                                                                                                               aGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGAc                                                                                                                 <  4:160874/59‑1 (MQ=255)
                                                                                                                                  cccAAAGGCAGGCCCTTATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGGCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCAGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAgg                    >  5:66420/1‑149 (MQ=255)
                                                                                                                                  cccAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAgg                    <  6:149226/149‑1 (MQ=255)
                                                                                                                                  cccAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAgg                    <  4:113071/149‑1 (MQ=255)
                                                                                                                                   ccAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGCCCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAggg                   >  5:278321/1‑149 (MQ=255)
                                                                                                                                    cAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAggg                   >  2:38153/1‑148 (MQ=255)
                                                                                                                                            ggcCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTg          <  2:56432/149‑1 (MQ=255)
                                                                                                                                               ccATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGt        >  3:172075/1‑148 (MQ=255)
                                                                                                                                                 ttatggatatggGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCaa     >  2:100876/2‑149 (MQ=255)
                                                                                                                                                  tatggatatggGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAAc    <  1:38153/149‑1 (MQ=255)
                                                                                                                                                     ggatatggGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAAccc  >  8:117786/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CCGCTGCAGGCCATCGCCCAGTGGCGGGCGGCCGAGGGAAAGTGC‑GGTAT‑AGATGGTCAGGCGCC‑GTTCGGGCAGCTCGCGTACGCGGGCGTAAAGCGCGTTGACGAAGGCGTTGGGTTTCCCCAGGCCCAAAGGCAGGCCCATATGGATATGGGCTGGCAGCCGGGACAAGACCTGCTCGACAGCCTGGTCGATGGAGCAGAGGTGCATGGGGCCTCCTGAAACATCCGTGGGTTCAGGGGTTGGACATGAGCTCAAGCGGGTTGGTTGCCTAGGGCAAACTCTGGTCAACCC  >  NC_002947/6015555‑6015848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑